MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Journal of biological chemistry 2014 imex curation Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. Accepted 2020-DEC-02 AT 14:21 GMT AT 14:21 GMT by LUANA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Journal of biological chemistry 2014 imex curation Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. uniprot for monkey FAK is not available Accepted 2020-DEC-02 AT 14:21 GMT AT 14:21 GMT by LUANA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Chlamydia effector TarP mimics the mammalian leucine-aspartic acid motif of paxillin to subvert the focal adhesion kinase during invasion. The Journal of biological chemistry 2014 imex curation Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecodh Escherichia coli (strain K12 / DH10B) 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Thwaites T., Nogueira AT., Campeotto I., Silva AP., Grieshaber SS., Carabeo RA. Accepted 2020-DEC-02 AT 14:21 GMT AT 14:21 GMT by LUANA The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH D8A4C15138AB0243 q824h6_chlcv DUF1547 domain-containing protein CCA_00170 protein protein Chlamydia caviae Chlamydophila caviae (strain ATCC VR-813 / DSM 19441 / GPIC) MTSPINNPSTTNVTTTTTSTPVVTTSTSFGGHVVSTTGTGALETTAQTVNTTAEQAVAQAESDSGAVIFTTQRDVSTTAPTTGGSASTATAASLLGTHILGTGRARTDSTSSSDSSISDTSTTSSTQDTGATGETQDAGATGETQDAGATGEAGGTQEASGDVDLGDLAGLRGSEAADGAERAEGPGGLPSMALPKYDPTDKASIIKFLSTPSVQAKLQTKAGHIVFMDEARGSFIFVRNGDWSTAESIAVTNGKTKEPITDVKDLEMCIAKFCVGYESMQADWSNNIQPRVAGQTGETGHYDHLLMSMKFKTTVLYGPWNSKESSSGYTPSVWRRGTKCDSGPIWGDVGGLKGINWNNVAKPDATAFSRETAAPAQPQPGPYTPPVINVNLGGISTNVNVTGGTTTTTVTTSPTPDMSEVDSTAGFDEIDGLSTQDESTETDIDDVETQSTSTQYEDTLHYESGGEEVDNLAPAPLPPGPPPPAKGGVNITGMPAANLQQILNNVREHLDTVYDQNGVHHEGNQDLGTVVRTSENGTYKPTVLLKNDQGDGGRGVQRSNDDDGGELGNILNRVREHLDVVYPESNEGEPIPVNQNLGDVIQAVESGNTPKPTQPEGVFVAKKVNLDGDGNIVGGNARTSSESRASNLMSAAGGEGAEGLEHLLPQLRSHLDDAFDQQGNLVGAPGTNVGSLIKAFQERTGSGGIVAPLPASVVTSSSAPQQSATVSDLPKAQTAETTTPGGSPDLHGAAKGVADSLSNLLQAATPSTTSTTVSSPAPRQETATSTSVAGTRGTATPTTGGSPSGIPGAAANVTATLSSVANKIALFEKGARLQEALDSADTESTQGKQLLEAARNTTTMLSKTLSKVTGSPPSPPQRHS 0058E981F0AA4130 paxi_chick Paxillin PXN protein protein chick Gallus gallus (Chicken) Chicken MDDLDALLADLESTTSHISKRPVFLTEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVIDPLDQWQPSVSRYGHQQPQSQSPIYSSSAKSSSASVPRDGLSSPSPRASEEEHVYSFPNKQKSAEPSPTMTSTSLGSNLSELDRLLLELNAVQHNPPSGFSADEVSRSPSLPNVTGPHYVIPESSSSAGGKAAPPTKEKPKRNGGRGIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQRVNASQQQTRISASSATRELDELMASLSDFKFMAQGKAGGSSSPPSTTPKPGSQLDTMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGVFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFINGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMGKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC 6450270D90B2DE84 1-173 2 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level phostyr397 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain Y y observed-ptm observed-ptm observed ptm observed-ptm 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain colocalization colocalization fig.2b q824h6_chlcv-ptk2-1 1 4 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 5 unspecified role unspecified role bait bait purified purified n-n biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q824H6:p.[Leu660Ala;Leu663_Leu664delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain LL AA Mon Mar 01 10:48:06 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q05397:p.[Leu660Ala;Leu663Ala;leu664ala]' chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association fig 5c ptk2-q824h6_chlcv-1 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q824H6:p.[Leu660Ala;Leu663_Leu664delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain LL AA Sun Dec 13 19:30:20 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q824H6:p.[keu640ala;leu643ala;leu644ala]' Mon Mar 01 11:07:51 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'Q824H6:p.[Leu640Ala;Leu643_Leu644delinsAlaAla]' 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region binding region binding-associated region binding region range range range range physical association physical association fig. 4f TarP-LD interaction with FAT was evaluated and directly compared with paxillin LD2 and TarP-mutLD. A positive control Cya18-Zip/Cya25-Zip was included. By measuring β-gal production, it was clear that Cya18-TarP-LD/Cya25-FAT or Cya18-Pax-LD2/Cya25-FAT-co-expressing bacteria exhibited activity that differed significantly from the negative control strain (Fig. 4F; p < 0.01 ANOVA and Tukey-Kramer post hoc test). Importantly, the Leu-to-Ala substitution mutant did not interact with FAT. ptk2-pxn-3 3 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region binding region binding-associated region binding region range range range range physical association physical association fig. 4f TarP-LD interaction with FAT was evaluated and directly compared with paxillin LD2 and TarP-mutLD. A positive control Cya18-Zip/Cya25-Zip was included. By measuring β-gal production, it was clear that Cya18-TarP-LD/Cya25-FAT or Cya18-Pax-LD2/Cya25-FAT-co-expressing bacteria exhibited activity that differed significantly from the negative control strain (Fig. 4F; p < 0.01 ANOVA and Tukey-Kramer post hoc test). Importantly, the Leu-to-Ala substitution mutant did not interact with FAT.