IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic T-lymphocyte epitopes. The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic T-lymphocyte epitopes. The Journal of infectious diseases 2010 imex curation Liu J., Sun Y., Qi J., Chu F., Wu H., Gao F., Li T., Yan J., Gao GF. Coronavirus - Interactions investigated in the context of Coronavirus the 2 PDBe entries, for what is meant to be the same CoV protein, use a different Uniprot entry for each, for some reason. The Q692E0 entry is for the TJF CoV strain. This publication states that the sequences of M and E SARS-CoV proteins were from strain TJF; GenBank accession no. AY654624. The sequence of the M protein gives a 99% match (one residue difference) with reviewed Uniprot entry P59596 and 100% match with several Trembl entries, including Q692E0. The entry P59596 has been used here, excluding one interaction for the x-ray diffraction experiment, when the interactors as given in the PDB entry have been used. gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu J., Sun Y., Qi J., Chu F., Wu H., Gao F., Li T., Yan J., Gao GF. Coronavirus - Interactions investigated in the context of Coronavirus the 2 PDBe entries, for what is meant to be the same CoV protein, use a different Uniprot entry for each, for some reason. The Q692E0 entry is for the TJF CoV strain. This publication states that the sequences of M and E SARS-CoV proteins were from strain TJF; GenBank accession no. AY654624. The sequence of the M protein gives a 99% match (one residue difference) with reviewed Uniprot entry P59596 and 100% match with several Trembl entries, including Q692E0. The entry P59596 has been used here, excluding one interaction for the x-ray diffraction experiment, when the interactors as given in the PDB entry have been used. gaof@im.ac.cn Accepted 2020-MAR-27 AT 11:07 GMT AT 11:07 GMT by LIVIAP The Journal of infectious diseases 2010 Only protein-protein interactions imex curation imex curation The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic T-lymphocyte epitopes. The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic T-lymphocyte epitopes. The Journal of infectious diseases 2010 imex curation Liu J., Sun Y., Qi J., Chu F., Wu H., Gao F., Li T., Yan J., Gao GF. Coronavirus - Interactions investigated in the context of Coronavirus the 2 PDBe entries, for what is meant to be the same CoV protein, use a different Uniprot entry for each, for some reason. The Q692E0 entry is for the TJF CoV strain. This publication states that the sequences of M and E SARS-CoV proteins were from strain TJF; GenBank accession no. AY654624. The sequence of the M protein gives a 99% match (one residue difference) with reviewed Uniprot entry P59596 and 100% match with several Trembl entries, including Q692E0. The entry P59596 has been used here, excluding one interaction for the x-ray diffraction experiment, when the interactors as given in the PDB entry have been used. gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu J., Sun Y., Qi J., Chu F., Wu H., Gao F., Li T., Yan J., Gao GF. Coronavirus - Interactions investigated in the context of Coronavirus the 2 PDBe entries, for what is meant to be the same CoV protein, use a different Uniprot entry for each, for some reason. The Q692E0 entry is for the TJF CoV strain. This publication states that the sequences of M and E SARS-CoV proteins were from strain TJF; GenBank accession no. AY654624. The sequence of the M protein gives a 99% match (one residue difference) with reviewed Uniprot entry P59596 and 100% match with several Trembl entries, including Q692E0. The entry P59596 has been used here, excluding one interaction for the x-ray diffraction experiment, when the interactors as given in the PDB entry have been used. gaof@im.ac.cn Accepted 2020-MAR-27 AT 11:07 GMT AT 11:07 GMT by LIVIAP The Journal of infectious diseases 2010 Only protein-protein interactions imex curation imex curation b2mg_human Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM DIP protein P61769 original sequence version: null DIP protein P61769 original sequence version: 138 DIP protein P61769 original sequence version: 134 DIP protein P61769 original sequence version: 135 DIP protein P61769 original sequence version: 116 DIP protein P61769 original sequence version: 60 DIP protein P61769 original sequence version: 79 DIP protein P61769 original sequence version: 87 DIP protein P61769 original sequence version: 93 DIP protein P61769 original sequence version: 94 DIP protein P61769 original sequence version: 91 DIP protein P61769 original sequence version: 106 DIP protein P61769 original sequence version: 111 AFD2DBEF07DCEF27 hlaa_human HLA class I histocompatibility antigen, A alpha chain HLA-A HLAA Human leukocyte antigen A protein protein human Homo sapiens Human MAVMAPRTLLLLLSGALALTQTWAGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRVDLGTLRGYYNQSEAGSHTIQIMYGCDVGSDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAAHEAEQLRAYLDGTCVEWLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQPTIPIVGIIAGLVLLGAVITGAVVAAVMWRRKSSDRKGGSYTQAASSDSAQGSDVSLTACKV DEDFCEC4450E0580 m1_i68a6 Matrix protein 1 M protein protein Influenza A virus (strain NT60) Influenza A virus (strain A/Northern Territory/60/1968 H3N2) Influenza A virus (strain A/NT/60/1968 H3N2) MSLLTEVETYVLSIVPSGPLKAEIAQRLEDVFAGKNTDLEALMEWLKTRPILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLYRKLKREITFHGAKEIALSYSAGALASCMGLIYNRMGAVTTEVAFGLVCATCEQIADSQHRSHRQMVTTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAAEAMEVASQARQMVQAMRAIGTHPRSSAGLKDDLLENLQAYQKRMGVQMQRFK 7317397B86DFC877 q692e0_sars Membrane protein M E1 glycoprotein Matrix glycoprotein Membrane glycoprotein protein protein SARS coronavirus TJF SARS coronavirus TJF MADNGTITVEKLKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ 439CD30C104CBFAC vemp_sars Envelope small membrane protein E sM 4 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYSRVKNLNSSEGVPDLLV A6D9A31652DDA194 vme1_sars Membrane protein M E1 glycoprotein Matrix glycoprotein Membrane glycoprotein 5 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MADNGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFLYIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFARTRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDLPKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQ 275C37085A0215A8 b2m-hla_a-4 2 4 unspecified role unspecified role neutral component neutral component purified purified p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain D H p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain I R p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain G W p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain I T p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain P A p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain G S p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain E Q p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain L P p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain R H partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain PI AV p.Gln86Gly variant variant certain certain sequence position certain certain certain sequence position certain Q G p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain Q H p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain I V p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain R H p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain D Y p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain E V p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain D E ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component Mn2_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association Table 2 HLA-A*0201 b2m-hla_a-10 1 4 unspecified role unspecified role neutral component neutral component purified purified p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain P A p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain D H p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain I R p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain G W p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain I T p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain G S p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain E Q p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain L P p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain R H partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain PI AV p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain Q E p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain Q H p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain I V p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain R H p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain D Y p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain E V p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain D E ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component Mn1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Md3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mn3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Mn2_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Md1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Md2_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association Figure 1A HLA-A*0201 b2m-hla_a-12 2 4 unspecified role unspecified role neutral component neutral component purified purified p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain D H p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain I R p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain G W p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain I T p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain G S p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain E Q p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain L P p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain R H partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain PI AV p.Gln86Gly variant variant certain certain sequence position certain certain certain sequence position certain Q G p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain Q H p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain I V p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain R H p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain D Y p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain E V p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain D E p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain P A ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component Md3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association Table 2 HLA-A*0201 b2m-hla_a-13 1 4 unspecified role unspecified role neutral component neutral component purified purified p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain D H p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain G W p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain I T p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain G S p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain E Q p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain L P p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain R H partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain PI AV p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain Q E p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain Q H p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain I V p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain I R p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain R H p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain D Y p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain E V p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain D E p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain P A ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component flu_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association Figure 1A HLA-A*0201 b2m-hla_a-14 1 4 unspecified role unspecified role neutral component neutral component purified purified p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain D H p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain G W p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain I T p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain G S p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain E Q p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain L P p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain R H partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain PI AV p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain Q E p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain Q H p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain I V p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain I R p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain R H p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain D Y p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain N K p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain E V p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain D E p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain P A ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component En1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain En4_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Ed5_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Ed2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association Figure 1A HLA-A*0201