MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. Cellular microbiology 2008 imex curation Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. hartland@unimelb.edu.au 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast two hybrid pooling two hybrid pooling approach two hybrid pooling nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. hartland@unimelb.edu.au Accepted 2020-DEC-02 AT 13:48 GMT AT 13:48 GMT by LUANA HeLa cDNA library Cellular microbiology 2008 Only protein-protein interactions imex curation imex curation A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. Cellular microbiology 2008 imex curation Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. hartland@unimelb.edu.au 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein array protein array anti tag immunost anti tag immunostaining anti tag immunost Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. Origins and range for the PDZ domains have been taken from PMID: 15901899 hartland@unimelb.edu.au Accepted 2020-DEC-02 AT 13:48 GMT AT 13:48 GMT by LUANA Cellular microbiology 2008 Only protein-protein interactions imex curation imex curation A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium. Cellular microbiology 2008 imex curation Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. hartland@unimelb.edu.au 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SF., Kelly M., McAlister A., Luck SN., Garcia EL., Hall RA., Robins-Browne RM., Frankel G., Hartland EL. hartland@unimelb.edu.au Accepted 2020-DEC-02 AT 13:48 GMT AT 13:48 GMT by LUANA Cellular microbiology 2008 Only protein-protein interactions imex curation imex curation sntg2_mouse Gamma-2-syntrophin Sntg2 Syntrophin-5 protein protein mouse Mus musculus Mouse MSAEGSQSLAAPRGRPSHLLVPARTKTALALLYDEGLENAYDVRLKLTKEVLTIQKQDVVCIGGAPPGANHRTVTLRRQPVGGLGLSIKGGAEHGVPVVISKIFKDQAADQTEMLFIGDAVLQVNGINVENATHEEVVHLLRNAGDDVTITVEYLREAPSFLKLPLGSPGPSSDHSSRASSPLFDSGLHLNGHCSHTAPSSPSSPIANEPKYEKRWLDTLSVPLSMARISRYKAGTEKLRSSALEVLALDGASTGVLQFSTAQDCADWLRSISTNISDLTLQHMKMANKCCSPCDQVVHMGWVNERLQGADNSQNFRPKFLALRGSSFYIFGAPPVSTLDWGRAERAYNLCEVLFKVHKFWLSDNYWLQANLYLGLQDFDCEDPRSYCFSVLANHGKSHIFSVELGSELAVWEKAFQRATFMEVQRTGSKTYLCSWQGETLCFTVDFALGFTCFDGKTKNVLWRFKFSQLKGSSDDGKTRVKLLFQNLDTKQIETKELEFQDLTAVLHCIHSFIAAKVASLDPVFMDSQSMARRYLCSS mint Adapter protein that binds to and probably organizes the subcellular localization of a variety of proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex 4DB2A0916336D379 snta1_mouse Alpha-1-syntrophin Snta1 Snt1 59 kDa dystrophin-associated protein A1 acidic component 1 Syntrophin-1 protein protein mouse Mus musculus Mouse MASGRRAPRTGLLELRCGAGSGAGGERWQRVLLSLAEDALTVSPADGEPGPEPEPAQLNGAAEPGAAPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQALKKTGKEVVLEVKYMKEVSPYFKNSAGGTSVGWDSPPASPLQRQPSSPGPQPRNLSEAKHVSLKMAYVSRRCTPTDPEPRYLEICAADGQDAVFLRAKDEASARSWAGAIQAQIGTFIPWVKDELQALLTATGTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLFYCSLPQSREALSRPTRTAPLIATSSAHRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGIQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAMLLRQPFEKLQMSSDDGTSLLFLDFGGAEGEIQLDLHSCPKTMVFIIHSFLSAKVTRLGLLA DIP protein Q61234 original sequence version: 86 161BA76CAF50AE96 nhrf3_mouse Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Pdzk1 Cap70 Nherf3 PDZ domain-containing protein 1 CFTR-associated protein of 70 kDa Sodium-hydrogen exchanger regulatory factor 3 Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 protein protein mouse Mus musculus Mouse MASTFNPRECKLSKQEGQNYGFFLRIEKDTDGHLIRVIEEGSPAEKAGLLDGDRVLRINGVFVDKEEHAQVVELVRKSGNSVTLLVLDGDSYEKAVKNQVDLKELDQSQREAALNDKKPGPGMNGAVEPCAQPRLCYLVKEGNSFGFSLKTIQGKKGVYLTDIMPQGVAMKAGVLADDHLIEVNGENVENASHEEVVEKVTKSGSRIMFLLVDKETARCHSEQKTQFKRETASLKLLPHQPRVVVIKKGSNGYGFYLRAGPEQKGQIIKDIEPGSPAEAAGLKNNDLVVAVNGKSVEALDHDGVVEMIRKGGDQTTLLVLDKEAESIYSLARFSPLLYCQSQELPNGSVKEGPAPIPAPLEATGSEPTEDAEGHKPKLCRLLKEDDSYGFHLNAIRGQPGSFVKEVQQGGPADKAGLENEDVIIEVNGENVQEEPYDRVVERIKSSGKHVTLLVCGKMAYSYFQAKKIPIVSSMAEPLVAGPDEKGETSAESEHDAHPAKDRTLSTASHSSSNSEDTEM mint A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism (By similarity). May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function 681B57F4E237BC28 dlg3_rat Disks large homolog 3 Dlg3 Dlgh3 Synapse-associated protein 102 PSD-95/SAP90-related protein 1 protein protein rat Rattus norvegicus (Rat) Rat MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKNTPKLNGSGPSWWPECTCTNRDWYEQASPAPLLVNPEALEPSLSVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL 34DA9C46C7BB96DB magi2_human Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 MAGI2 KIAA0705 ACVRINP1 Membrane-associated guanylate kinase inverted 2 Atrophin-1-interacting protein 1 Atrophin-1-interacting protein A AIP1 protein protein human Homo sapiens Human MSKSLKKKSHWTSKVHESVIGRNPEGQLGFELKGGAENGQFPYLGEVKPGKVAYESGSKLVSEELLLEVNETPVAGLTIRDVLAVIKHCKDPLRLKCVKQGGIVDKDLRHYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRPHKEGEVPGVDYIFITVEDFMELEKSGALLESGTYEDNYYGTPKPPAEPAPLLLNVTDQILPGATPSAEGKRKRNKSVSNMEKASIEPPEEEEEERPVVNGNGVVVTPESSEHEDKSAGASGEMPSQPYPAPVYSQPEELKEQMDDTKPTKPEDNEEPDPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHNMPHTELGTKPLQAPGFREKPLFTRDASQLKGTFLSTTLKKSNMGFGFTIIGGDEPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLGHTHADVVKLFQSVPIGQSVNLVLCRGYPLPFDPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYISRTSQSVPDITDRPPHSLHSMPTDGQLDGTYPPPVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDIQGCPGLCEGDLIVEINQQNVQNLSHTEVVDILKDCPIGSETSLIIHRGGFFSPWKTPKPIMDRWENQGSPQTSLSAPAIPQNLPFPPALHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTSFRMDSSGPDYKELDVHLRRMESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVIDLMHHAARNGQVNLTVRRKVLCGGEPCPENGRSPGSVSTHHSSPRSDYATYTNSNHAAPSSNASPPEGFASHSLQTSDVVIHRKENEGFGFVIISSLNRPESGSTITVPHKIGRIIDGSPADRCAKLKVGDRILAVNGQSIINMPHADIVKLIKDAGLSVTLRIIPQEELNSPTSAPSSEKQSPMAQQSPLAQQSPLAQPSPATPNSPIAQPAPPQPLQLQGHENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYQQPPPLDYRQPPLLDYRQHSPDTRQYPLSDYRQPQDFDYFTVDMEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVRLLLKRGTGQVPEYDEPAPWSSPAAAAPGLPEVGVSLDDGLAPFSPSHPAPPSDPSHQISPGPTWDIKREHDVRKPKELSACGQKKQRLGEQRERSASPQRAARPRLEEAPGGQGRPEAGRPASEARAPGLAAADAADAARAGGKEAPRAAAGSELCRREGPGAAPAFAGPGGGGSGALEAEGRAGARAGPRPGPRPPGGAPARKAAVAPGPWKVPGSDKLPSVLKPGASAASR 93E170D070A70A9C sntb1_mouse Beta-1-syntrophin Sntb1 Snt2b1 59 kDa dystrophin-associated protein A1 basic component 1 Syntrophin-2 protein protein mouse Mus musculus Mouse MAVAAAAVAAPAGGGGARAQRSGLLEVLVRDRWHKVLVNLSEDALVLSCEEGAAAYNGIGAATNGSFCRGSGTGHPVPGVAQAPDSPAGVRTAFTDLPEQVPESISNQKRGVKVLKQELGGLGISIKGGKENKMPILISKIFKGLAADQTQALYVGDAILSVNGADLRDATHDEAVQALKRAGKEVLLEVKYMREATPYVKKGSPVSEIGWETPPPESPRLGGGSAEPLSSQSFSFHRDRKSIPLKMCYVTRNMTLADPENRQLEIHSPDAKHTVILRSKDSATAQAWFSAIHSNAGDLLTRVVADIREQLGKTGIAGSREIRHLGWLAEKVPGESEKQWKPALVVLTEKDLLIYDSMPRRKEAWFSPVHSYPLLATRLVHSGPGKGSPQAGMDLSFATRTGTKQGIETHLFRAEISRDLSHWTRSIVQGCHNSAELTAEITTACTYRNQECRLTIHYDNGFSISTEPQDGAFPKTIIQSPYEKLKMSSDDGIRMLYLDFGGKEGEIQLDLHSCPKPIVFIIHSFLSAKITRLGLVA 91A5E4DD0A97A665 nhrf4_mouse Na(+)/H(+) exchange regulatory cofactor NHE-RF4 Pdzd3 Pdzk2 Nherf4 PDZ domain-containing protein 3 PDZ domain-containing protein 2 Natrium-phosphate cotransporter IIa C-terminal-associated protein 2 Sodium-hydrogen exchanger regulatory factor 4 protein protein mouse Mus musculus Mouse MEAAADLRDTALLTLKFKFNPRLGIDNPVLSLAEDQDQSDPWNLHRPRFCLLSKEEEKTFGFHLQQHLGKADHVVCRVDPGTSAQRQGLREGDRILAVNNNIVAHEDHAVVVRYIRASGPRVLLTVLAQHVHDVARVLQGSDAFLCPTLPSGVRPRLCHVVKDEGGFGFSVTHGSRGPFWLVLSAGGAAERAGVPPGARLLEVNGASVEKLTYNQLNRKLWQSGDQVTLLVAGLEVEEQCHQLGMPLAAPLAEGWALPAKPRCLNIEKGPEGFGFLLREEKGLDGRLGQFLWDVDPGLPADKAGMKAGDRLVAVAGESVDGLGHEETVSRIRAQGSCVSLIVVDPEADRFFSMVRLSPLLFLENTEIAAPPLAETKDLPVEDTVEPSGLAGSCQCFLYPGPGGGYGFRLCCVASGPCLFISQVTPGGSTARAGLQVGDTVLEVNGYPVGGDSELDRLQQLTEAEPPLCLKLGARNPQGLEAWISLESGEDWTLASELL mint Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation CD979649B08E475A magi3_human Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 MAGI3 KIAA1634 Membrane-associated guanylate kinase inverted 3 protein protein human Homo sapiens Human MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASIFEESHVPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQSLLQKNVSKRDPPSSHGHSNKKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGENKSCQVSTRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYTGSNAEQIPDGKEKSDVIRKDAKQNQLEKSRTRSPEKKIKRMVEKSLPSKMTNKTTSKEVSENEKGKKVTTGETSSSNDKIGENVQLSEKRLKQEPEEKVVSNKTEDHKGKELEAADKNKETGRFKPESSSPVKKTLITPGPWKVPSGNKVTGTIGMAEKRQ 9C2B604D9B1B1B97 sntb2_human Beta-2-syntrophin SNT2B2 SNTB2 D16S2531E SNTL 59 kDa dystrophin-associated protein A1 basic component 2 Syntrophin-3 Syntrophin-like protein protein human Homo sapiens Human MRVAAATAAAGAGPAMAVWTRATKAGLVELLLRERWVRVVAELSGESLSLTGDAAAAELEPALGPAAAAFNGLPNGGGAGDSLPGSPSRGLGPPSPPAPPRGPAGEAGASPPVRRVRVVKQEAGGLGISIKGGRENRMPILISKIFPGLAADQSRALRLGDAILSVNGTDLRQATHDQAVQALKRAGKEVLLEVKFIREVTPYIKKPSLVSDLPWEGAAPQSPSFSGSEDSGSPKHQNSTKDRKIIPLKMCFAARNLSMPDLENRLIELHSPDSRNTLILRCKDTATAHSWFVAIHTNIMALLPQVLAELNAMLGATSTAGGSKEVKHIAWLAEQAKLDGGRQQWRPVLMAVTEKDLLLYDCMPWTRDAWASPCHSYPLVATRLVHSGSGCRSPSLGSDLTFATRTGSRQGIEMHLFRVETHRDLSSWTRILVQGCHAAAELIKEVSLGCMLNGQEVRLTIHYENGFTISRENGGSSSILYRYPFERLKMSADDGIRNLYLDFGGPEGELTMDLHSCPKPIVFVLHTFLSAKVTRMGLLV 53A9F3340DC024ED lin7c_human Protein lin-7 homolog C Mammalian lin-seven protein 3 Vertebrate lin-7 homolog 3 LIN7C MALS3 VELI3 protein protein human Homo sapiens Human MAALGEPVRLERDICRAIELLEKLQRSGEVPPQKLQALQRVLQSEFCNAVREVYEHVYETVDISSSPEVRANATAKATVAAFAASEGHSHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRIIPGGIADRHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGKVKLVVRYTPKVLEEMESRFEKMRSAKRRQQT 7410FBFA3BD24F45 dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein P31016 original sequence version: 86 DIP protein P31016-1 original sequence version: 148 7922D4E8E0F9AD85 snx27_human Sorting nexin-27 SNX27 KIAA0488 My014 protein protein human Homo sapiens Human MADEDGEGIHPSAPHRNGGGGGGGGSGLHCAGNGGGGGGGPRVVRIVKSESGYGFNVRGQVSEGGQLRSINGELYAPLQHVSAVLPGGAADRAGVRKGDRILEVNHVNVEGATHKQVVDLIRAGEKELILTVLSVPPHEADNLDPSDDSLGQSFYDYTEKQAVPISVPRYKHVEQNGEKFVVYNVYMAGRQLCSKRYREFAILHQNLKREFANFTFPRLPGKWPFSLSEQQLDARRRGLEEYLEKVCSIRVIGESDIMQEFLSESDENYNGVSDVELRVALPDGTTVTVRVKKNSTTDQVYQAIAAKVGMDSTTVNYFALFEVISHSFVRKLAPNEFPHKLYIQNYTSAVPGTCLTIRKWLFTTEEEILLNDNDLAVTYFFHQAVDDVKKGYIKAEEKSYQLQKLYEQRKMVMYLNMLRTCEGYNEIIFPHCACDSRRKGHVITAISITHFKLHACTEEGQLENQVIAFEWDEMQRWDTDEEGMAFCFEYARGEKKPRWVKIFTPYFNYMHECFERVFCELKWRKENIFQMARSQQRDVAT 1AA2265E463CB8DA sc24b_human Protein transport protein Sec24B SEC24B SEC24-related protein B protein protein human Homo sapiens Human MSAPAGSSHPAASARIPPKFGGAAVSGAAAPAGPGAGPAPHQQNGPAQNQMQVPSGYGLHHQNYIAPSGHYSQGPGKMTSLPLDTQCGDYYSALYTVPTQNVTPNTVNQQPGAQQLYSRGPPAPHIVGSTLGSFQGAASSASHLHTSASQPYSSFVNHYNSPAMYSASSSVASQGFPSTCGHYAMSTVSNAAYPSVSYPSLPAGDTYGQMFTSQNAPTVRPVKDNSFSGQNTAISHPSPLPPLPSQQHHQQQSLSGYSTLTWSSPGLPSTQDNLIRNHTGSLAVANNNPTITVADSLSCPVMQNVQPPKSSPVVSTVLSGSSGSSSTRTPPTANHPVEPVTSVTQPSELLQQKGVQYGEYVNNQASSAPTPLSSTSDDEEEEEEDEEAGVDSSSTTSSASPMPNSYDALEGGSYPDMLSSSASSPAPDPAPEPDPASAPAPASAPAPVVPQPSKMAKPFGYGYPTLQPGYQNATAPLISGVQPSNPVYSGFQQYPQQYPGVNQLSSSIGGLSLQSSPQPESLRPVNLTQERNILPMTPVWAPVPNLNADLKKLNCSPDSFRCTLTNIPQTQALLNKAKLPLGLLLHPFRDLTQLPVITSNTIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFMYNPLTRSYGEPHKRPEVQNSTVEFIASSDYMLRPPQPAVYLFVLDVSHNAVEAGYLTILCQSLLENLDKLPGDSRTRIGFMTFDSTIHFYNLQEGLSQPQMLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPNMFTNTRETHSALGPALQAAFKLMSPTGGRVSVFQTQLPSLGAGLLQSREDPNQRSSTKVVQHLGPATDFYKKLALDCSGQQTAVDLFLLSSQYSDLASLACMSKYSAGCIYYYPSFHYTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIRCTKGLSMHTFHGNFFVRSTDLLSLANINPDAGFAVQLSIEESLTDTSLVCFQTALLYTSSKGERRIRVHTLCLPVVSSLADVYAGVDVQAAICLLANMAVDRSVSSSLSDARDALVNAVVDSLSAYGSTVSNLQHSALMAPSSLKLFPLYVLALLKQKAFRTGTSTRLDDRVYAMCQIKSQPLVHLMKMIHPNLYRIDRLTDEGAVHVNDRIVPQPPLQKLSAEKLTREGAFLMDCGSVFYIWVGKGCDNNFIEDVLGYTNFASIPQKMTHLPELDTLSSERARSFITWLRDSRPLSPILHIVKDESPAKAEFFQHLIEDRTEAAFSYYEFLLHVQQQICK 0E08B982D0982F84 nhrf2_human Na(+)/H(+) exchange regulatory cofactor NHE-RF2 SLC9A3R2 NHERF2 Tyrosine kinase activator protein 1 SRY-interacting protein 1 Solute carrier family 9 isoform A3 regulatory factor 2 NHE3 kinase A regulatory protein E3KARP Sodium-hydrogen exchanger regulatory factor 2 protein protein human Homo sapiens Human MAAPEPLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQLTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPRLCHLRKGPQGYGFNLHSDKSRPGQYIRSVDPGSPAARSGLRAQDRLIEVNGQNVEGLRHAEVVASIKAREDEARLLVVDPETDEHFKRLRVTPTEEHVEGPLPSPVTNGTSPAQLNGGSACSSRSDLPGSDKDTEDGSAWKQDPFQESGLHLSPTAAEAKEKARAMRVNKRAPQMDWNRKREIFSNF 4F5D341590D22ED7 pdz11_human PDZ domain-containing protein 11 PDZD11 PDZK11 AIPP1 ATPase-interacting PDZ protein PISP Plasma membrane calcium ATPase-interacting single-PDZ protein HSPC227 UNQ6486/PRO21335 protein protein human Homo sapiens Human MDSRIPYDDYPVVFLPAYENPPAWIPPHERVHHPDYNNELTQFLPRTITLKKPPGAQLGFNIRGGKASQLGIFISKVIPDSDAHRAGLQEGDQVLAVNDVDFQDIEHSKAVEILKTAREISMRVRFFPYNYHRQKERTVH 601216B821A36DCE tcof_human Treacle protein TCOF1 Treacher Collins syndrome protein protein protein human Homo sapiens Human MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV DIP protein Q13428 original sequence version: 135 6348306D0479790B d2tjz4_citri espI ROD_03401 protein protein Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 MNIQPNIHSGITTQNNQQHHHAEQVPVSSSIPRSDLPPNCEAGFVVHIPEDIQQHVPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSLGKKALDTFGLFNVTQMALEIPSSVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEIGFNFKDLPGPLKNALTNSSISATASTVAPTPNDPMPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGTSPLQATLGLEFGAHFKWDLDNPNTKYAILTNAAANAIGAAGGFAVSKVPGIDPMLSPHVGAMLGQAAGHAVQCNTPGLKPDTILWWAGATFGAADLNKAEFDKVRFTDYPRIWFHAREGALFPNKQDIARVTGADIKAMEEGVPVGHQHPKPEDVVIDIEGGNSPHHNPSNYVDTFEIIQETRV 77E0E76A3D7D46D5 nhrf1_rabit Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKVGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPDTDEQFRKLGVQIRGELLRAQAGPEQAGPPAAPGEQGPAGENEPREVEKSHPERRELRPRLCAMKKGPNGYGFNLHSDKSRPGQFIRAVDPDSPAEASGLREQDRIVEVNGVCVEGKQHGDVVTAIKAGGDEAKLLVVDKETDEFFKKCKVVPSSEHLNGPLPEPFTNGEIQKNNPETLAPAASESPRPALARSASSDTSEELASQDSPKKEDSTAPSSTSSSSDPILDFSISLAVAKERAHQKRSSRRAPQMDWSEKKELFSNL Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli mint F55E6D6FFA01B991 snta1_human Alpha-1-syntrophin 59 kDa dystrophin-associated protein A1 acidic component 1 Pro-TGF-alpha cytoplasmic domain-interacting protein 1 Syntrophin-1 SNTA1 SNT1 protein protein human Homo sapiens Human MASGRRAPRTGLLELRAGAGSGAGGERWQRVLLSLAEDVLTVSPADGDPGPEPGAPREQEPAQLNGAAEPGAGPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQVLKKTGKEVVLEVKYMKDVSPYFKNSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFSEAKHMSLKMAYVSKRCTPNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKDELQALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSLPETREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTIVFIIHSFLSAKVTRLGLLA DIP protein Q13424 original sequence version: 78 C07DA5F21C775CF8 b7ur60_eco27 E2348C_1442 protein protein eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVVHIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNVSQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPLKNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGTTLGLADLNKAEFGEARFTDYPRIWWHAREGAIFPNKADIEHATGADIRAMEEGVSVGQRHPNPEDVVINIESNNSPHHNPSNYVDTVDIIQETRV 4ABDAE5CBBD4EA55 dlg1_rat Disks large homolog 1 Dlg1 Synapse-associated protein 97 Dlgh1 protein protein rat Rattus norvegicus (Rat) Rat MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESPVVLPSTPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKAFRKNHEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKAAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLRETMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYHEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL DIP protein Q62696 original sequence version: 129 DIP protein Q62696 original sequence version: 155 18CEBD31DD0CAF8B b7ur60_eco27-snta1-1 1 22 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-lin7c-1 1 13 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-pdzd11-1 1 18 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-snx27-1 1 15 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-sec24b-1 1 16 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-tcof1-1 1 19 unspecified role unspecified role prey prey cdna library cdna library binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association table 1 To identify potential eukaryotic interacting partners of EspI, we performed a Y2HS screen of a pretransformed HeLa cDNA library using the full-length EspI from EPEC E2348/69 as bait. b7ur60_eco27-snta1-2 3 22 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain B7UR60:p.[Glu437Asp;Val440Leu] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L physical association physical association fig.1a, fig. 2 b7ur60_eco27-sec24b-2 3 16 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain B7UR60:p.[Glu437Asp;Val440Leu] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L physical association physical association fig.1a, fig. 2 b7ur60_eco27-tcof1-2 3 19 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed B7UR60:p.[Glu437Asp;Val440Leu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L physical association physical association fig.1a, fig. 2 b7ur60_eco27-pdzd11-2 3 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed B7UR60:p.[Glu437Asp;Val440Leu] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.1a, fig. 2 b7ur60_eco27-lin7c-2 3 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed B7UR60:p.[Glu437Asp;Val440Leu] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.1a, fig. 2 b7ur60_eco27-snx27-2 3 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed B7UR60:p.[Glu437Asp;Val440Leu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E D certain certain sequence position certain certain certain sequence position certain V L binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.1a, fig. 2 magi2-b7ur60_eco27-1 2 8 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 magi3-b7ur60_eco27-1 2 11 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 slc9a3r1-b7ur60_eco27-1 2 21 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 slc9a3r2-b7ur60_eco27-2 2 17 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 dlg4-b7ur60_eco27-1 2 14 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 dlg1-b7ur60_eco27-1 2 24 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 dlg3-b7ur60_eco27-1 2 7 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 snta1-b7ur60_eco27-1 2 5 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 sntb1-b7ur60_eco27-1 2 9 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 sntb2-b7ur60_eco27-1 2 12 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 sntg2-b7ur60_eco27-1 2 4 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 pdzk1-b7ur60_eco27-1 2 6 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 pdzd3-b7ur60_eco27-1 2 10 unspecified role unspecified role bait bait purified purified PDZ domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 23 predetermined predetermined participant predetermined anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role prey prey purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction fig. 1b,1c table 2 espi-lin7c-1 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a espi-snx27-1 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a espi-pdzd11-1 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a espi-snta1-1 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a espi-sec24b-1 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a espi-sec24b-2 3 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 19 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association fig.6a