UniProt UniProt http://www.uniprot.org Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Protein expression and purification 2007 imex curation Chu CH., Cheng D. dong.cheng@bms.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Chu CH., Cheng D. dong.cheng@bms.com Accepted 2020-OCT-26 AT 08:36 GMT AT 08:36 GMT by KALPANAP Protein expression and purification 2007 Only protein-protein interactions imex curation imex curation Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Protein expression and purification 2007 imex curation Chu CH., Cheng D. dong.cheng@bms.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti tag coip anti tag coimmunoprecipitation anti tag coip weight by staining molecular weight estimation by staining weight by staining Chu CH., Cheng D. dong.cheng@bms.com Accepted 2020-OCT-26 AT 08:36 GMT AT 08:36 GMT by KALPANAP Protein expression and purification 2007 Only protein-protein interactions imex curation imex curation Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC). Protein expression and purification 2007 imex curation Chu CH., Cheng D. dong.cheng@bms.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Chu CH., Cheng D. The complex was first purified by anti-Myc column chromatography dong.cheng@bms.com Accepted 2020-OCT-26 AT 08:36 GMT AT 08:36 GMT by KALPANAP Protein expression and purification 2007 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method biotinate biotinate biotin biotinate anion 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mcca_human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial MCCC1 MCCA 3-methylcrotonyl-CoA carboxylase 1 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha 3-methylcrotonyl-CoA carboxylase biotin-containing subunit protein protein human Homo sapiens Human MAAASAVSVLLVAAERNRWHRLPSLLLPPRTWVWRQRTMKYTTATGRNITKVLIANRGEIACRVMRTAKKLGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAELCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGYPVMIKAVRGGGGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFCFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNFMPVAGPLVHLSTPRADPSTRIETGVRQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVGLHTNIDFLLNLSGHPEFEAGNVHTDFIPQHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAHDQFSPFSSSSGRRLNISYTRNMTLKDGKNNVAIAVTYNHDGSYSMQIEDKTFQVLGNLYSEGDCTYLKCSVNGVASKAKLIILENTIYLFSKEGSIEIDIPVPKYLSSVSSQETQGGPLAPMTGTIEKVFVKAGDKVKAGDSLMVMIAMKMEHTIKSPKDGTVKKVFYREGAQANRHTPLVEFEEEESDKRESE B84AD23806035A40 mccb_human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial MCCC2 MCCB 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit 3-methylcrotonyl-CoA carboxylase 2 protein protein human Homo sapiens Human MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM DIP protein Q9HCC0 original sequence version: null 8E3D401AF52DC7D2 3-methylbut-2-enoyl-coa 3-methylbut-2-enoyl-CoA 3,3-dimethacrylyl-CoA 3,3-dimethacrylyl-coenzyme A 3-Methylbut-2-enoyl-CoA 3-Methylcrotonoyl-CoA 3-Methylcrotonyl-CoA beta,beta-dimethacrylyl-CoA beta,beta-dimethacrylyl-coenzyme A Dimethylacryloyl-CoA DMA-CoA S-(3-Methyl-crotonoyl)-coenzym-A S-(3-methylcrotonoyl)-coenzyme-A 3'-phosphoadenosine 5'-{3-[(3R)-3-hydroxy-2,2-dimethyl-4-{[3-({2-[(3-methylbut-2-enoyl)sulfanyl]ethyl}amino)-3-oxopropyl]amino}-4-oxobutyl] dihydrogen diphosphate} small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mccc1-mccc2-1 2 7 weight by staining molecular weight estimation by staining weight by staining anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 weight by staining molecular weight estimation by staining weight by staining anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2A, 2B mccc1-1 2 7 weight by staining molecular weight estimation by staining weight by staining anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 weight by staining molecular weight estimation by staining weight by staining anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4A, 4B mccc2-mccc1-2 3 7 unspecified role unspecified role neutral component neutral component purified purified c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role neutral component neutral component purified purified c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Fig. 6 3_methylbut_2_enoyl_coa-1 1 26 enzyme enzyme unspecified role unspecified role purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 enzyme target enzyme target substrate substrate unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig. 2C, Fig 3 mccc2-mccc1-1 3-methylcrotonyl-CoA carboxylase 1 complex 3-methylcrotonyl-CoA:carbon dioxide ligase complex MCCC complex Mitochondrial methylcrotonoyl-CoA carboxylase complex MCCC1:MCCC2 Mitochondrial methylcrotonyl-CoA carboxylase complex 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 cofactor cofactor coenzyme mccc1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme enzyme 6 enzyme enzyme biotin binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 31 29 35 33 41 37 39 43 45 47 52 49 direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism. The biotin carboxylase component catalyzes the Mg-ATP-dependent carboxylation of the N1' atom of the biotin cofactor, using bicarbonate as the CO2 donor. The carboxyltransferase component then catalyzes the CO2 transfer from carboxybiotin to the gamma carbon of the alpha-beta unsaturated acid, 3-methylcrotonyl-CoA. Molecular weight 500-800 kDa Heterododecamer 3-methylcrotonoyl-CoA carboxylase deficiency (MCCD) [Orphanet:6]: an autosomal recessive inherited disorder of leucine metabolism characterized by a highly variable clinical picture ranging from metabolic crisis in infancy to asymptomatic adults. There is a characteristic organic aciduria with massive excretion of 3- hydroxyisovaleric acid and 3-methylcrotonylglycine, usually in combination with a severe secondary carnitine deficiency. Accepted 2020-NOV-04 by BMELDAL