IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the mid-region of tropomyosin: bending and binding sites for actin. Structure of the mid-region of tropomyosin: bending and binding sites for actin. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Brown JH., Zhou Z., Reshetnikova L., Robinson H., Yammani RD., Tobacman LS., Cohen C. ccohen@brandeis.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Brown JH., Zhou Z., Reshetnikova L., Robinson H., Yammani RD., Tobacman LS., Cohen C. ccohen@brandeis.edu Accepted 2020-APR-30 AT 15:40 BST AT 15:40 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation tpm1_rat Tropomyosin alpha-1 chain Tpm1 Alpha-tm Tpma Tropomyosin-1 Alpha-tropomyosin protein protein rat Rattus norvegicus (Rat) Rat MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI DIP protein P04692 original sequence version: 60 E25609F597A72F4D tpm1-1 1 2 unspecified role unspecified role neutral component neutral component purified purified tropomyosin's mid-region tropomyosin's mid-region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminal GC4 tag C-terminal GC4 tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminal GC4-tag from yeas GC4 (UniProt P03069): 255-280 ecoli Escherichia coli (strain K12) difference in PDB and UniProt sequence: Met at position 88. Is that an N-terminal tag? How to annotate it? 2 unspecified role unspecified role neutral component neutral component purified purified tropomyosin's mid-region tropomyosin's mid-region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminal GC4-tag C-terminal GC4-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminal GC4-tag from yeas GC4 (UniProt P03069): 255-280 ecoli Escherichia coli (strain K12) difference in PDB and UniProt sequence: Met at position 88. Is that an N-terminal tag? How to annotate it? 5 8 direct interaction direct interaction 1/a Rwork=0.256 Rfree=0.296 2.3Å