IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NMR structure of a bifunctional rhodamine labeled N-domain of troponin C complexed with the regulatory "switch" peptide from troponin I: implications for in situ fluorescence studies in muscle fibers. NMR structure of a bifunctional rhodamine labeled N-domain of troponin C complexed with the regulatory "switch" peptide from troponin I: implications for in situ fluorescence studies in muscle fibers. Biochemistry 2003 imex curation Mercier P., Ferguson RE., Irving M., Corrie JE., Trentham DR., Sykes BD. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mercier P., Ferguson RE., Irving M., Corrie JE., Trentham DR., Sykes BD. Accepted 2020-MAY-22 AT 09:39 BST AT 09:39 BST by ORCHARD Biochemistry 2003 Only protein-protein interactions imex curation imex curation tnnc2_chick Troponin C, skeletal muscle TNNC2 protein protein chick Gallus gallus (Chicken) Chicken MASMTDQQAEARAFLSEEMIAEFKAAFDMFDADGGGDISTKELGTVMRMLGQNPTKEELDAIIEEVDEDGSGTIDFEEFLVMMVRQMKEDAKGKSEEELANCFRIFDKNADGFIDIEELGEILRATGEHVTEEDIEDLMKDSDKNNDGRIDFDEFLKMMEGVQ C4E1D9F40FFED3BC tnni2_rabit Troponin I, fast skeletal muscle TNNI2 Troponin I, fast-twitch isoform protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MGDEEKRNRAITARRQHLKSVMLQIAATELEKEEGRREAEKQNYLAEHCPPLSLPGSMAEVQELCKQLHAKIDAAEEEKYDMEIKVQKSSKELEDMNQKLFDLRGKFKRPPLRRVRMSADAMLKALLGSKHKVCMDLRANLKQVKKEDTEKERDLRDVGDWRKNIEEKSGMEGRKKMFESES 5BE12DBB6C90FFCF tnni2-1 1 2 unspecified role unspecified role neutral component neutral component purified purified N-Domain N-Domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P02588:p.Lys56Cys Glu56Cys mutation mutation certain certain sequence position certain certain certain sequence position certain K C Tue Jun 30 15:58:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu56Cys' P02588:p.Glu64Cys Glu63Cys mutation mutation certain certain sequence position certain certain certain sequence position certain E C Tue Jun 30 18:50:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu64Cys' bifunctional rhodamine label1 bifunctional rhodamine label1 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain bifunctional rhodamine with both methyl groups 13C labeled and attached to mutated residue C56 of sNTnC bifunctional rhodamine label2 bifunctional rhodamine label2 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain bifunctional rhodamine with both methyl groups 13C labeled and attached to mutated residue C63 of sNTnC 13C radiolabelled 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15N radiolabelled 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component purified purified Regulatory “Switch” Peptide Regulatory “Switch” Peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15N radiolabelled 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13C radiolabelled 13C radiolabelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P02643:p.Lys124Arg Lys124Arg mutation mutation certain certain sequence position certain certain certain sequence position certain K R not specified in paper, difference in PDB and UniProt sequence Tue Jun 30 16:30:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys124Arg' ecoli Escherichia coli (strain K12) 14 6 direct interaction direct interaction Figure 7/A