DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the spinach cytochrome b6 f complex at 3.6 Å resolution. Cryo-EM structure of the spinach cytochrome b6 f complex at 3.6 Å resolution. Nature 2019 imex curation Malone LA., Qian P., Mayneord GE., Hitchcock A., Farmer DA., Thompson RF., Swainsbury DJK., Ranson NA., Hunter CN., Johnson MP. matt.johnson@sheffield.ac.uk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation spiol Spinacia oleracea (Spinach) Spinach 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single predetermined predetermined participant predetermined Malone LA., Qian P., Mayneord GE., Hitchcock A., Farmer DA., Thompson RF., Swainsbury DJK., Ranson NA., Hunter CN., Johnson MP. matt.johnson@sheffield.ac.uk Accepted 2020-MAR-03 AT 14:53 GMT AT 14:53 GMT by ORCHARD Nature 2019 imex curation Cryo-EM structure of the spinach cytochrome b6 f complex at 3.6 Å resolution. Cryo-EM structure of the spinach cytochrome b6 f complex at 3.6 Å resolution. Nature 2019 imex curation Malone LA., Qian P., Mayneord GE., Hitchcock A., Farmer DA., Thompson RF., Swainsbury DJK., Ranson NA., Hunter CN., Johnson MP. matt.johnson@sheffield.ac.uk 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation spiol Spinacia oleracea (Spinach) Spinach molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Malone LA., Qian P., Mayneord GE., Hitchcock A., Farmer DA., Thompson RF., Swainsbury DJK., Ranson NA., Hunter CN., Johnson MP. matt.johnson@sheffield.ac.uk Accepted 2020-MAR-03 AT 14:53 GMT AT 14:53 GMT by ORCHARD Nature 2019 imex curation petn_spiol Cytochrome b6-f complex subunit 8 petN ycf6 Cytochrome b6-f complex subunit PetN Cytochrome b6-f complex subunit VIII protein protein spiol Spinacia oleracea (Spinach) Spinach MDIVSLAWAALMVVFTFSLSLVVWGRSGL 2B1641516534C166 petg_spiol Cytochrome b6-f complex subunit 5 Cytochrome b6-f complex subunit PetG Cytochrome b6-f complex subunit V petG petE protein protein spiol Spinacia oleracea (Spinach) Spinach MIEVFLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDL A98EA4D30CB983C5 petm_spiol Cytochrome b6-f complex subunit 7 petM Cytochrome b6-f complex subunit PetM Cytochrome b6-f complex subunit VII protein protein spiol Spinacia oleracea (Spinach) Spinach NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE 3CFD042EEDEBF602 petl_spiol Cytochrome b6-f complex subunit 6 petL Cytochrome b6-f complex subunit PetL Cytochrome b6-f complex subunit VI protein protein spiol Spinacia oleracea (Spinach) Spinach MFTLTSYFGFLLAALTITSALFIGLNKIRLI BEE4294AF4781439 cyb6_spiol Cytochrome b6 petB protein protein spiol Spinacia oleracea (Spinach) Spinach MSKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCLGGITLTCFLVQVATGFAMTFYYRPTVTDAFASVQYIMTEVNFGWLIRSVHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVVLGVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLRGSASVGQSTLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL A4518BF715B62F7D ucria_spiol Cytochrome b6-f complex iron-sulfur subunit, chloroplastic petC Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein Rieske iron-sulfur protein protein protein spiol Spinacia oleracea (Spinach) Spinach MASFTLSSATPSQLCSSKNGMFAPSLALAKAGRVNVLISKERIRGMKLTCQATSIPADNVPDMQKRETLNLLLLGALSLPTGYMLLPYASFFVPPGGGAGTGGTIAKDALGNDVIAAEWLKTHAPGDRTLTQGLKGDPTYLVVESDKTLATFGINAVCTHLGCVVPFNAAENKFICPCHGSQYNNQGRVVRGPAPLSLALAHCDVDDGKVVFVPWTETDFRTGEAPWWSA FA2C54B4C6586059 cyf_spiol Cytochrome f petA protein protein spiol Spinacia oleracea (Spinach) Spinach MQTINTFSWIKEQITRSISISLILYIITRSSIANAYPIFAQQGYENPREATGRIVCANCHLANKPVDIEVPQAVLPDTVFEAVVRIPYDMQLKQVLANGKKGGLNVGAVLILPEGFELAPPDRISPEMKEKMGNLSFQSYRPNKQNILVIGPVPGQKYSEITFPILAPDPATKKDVHFLKYPIYVGGNRGRGQIYPDGSKSNNTVYNSTATGIVKKIVRKEKGGYEINIADASDGREVVDIIPRGPELLVSEGESIKLDQPLTSNPNVGGFGQGDAEVVLQDPLRIQGLLFFFASVILAQIFLVLKKKQFEKVQLSEMNF 33B9FE0D02F19C36 petd_spiol Cytochrome b6-f complex subunit 4 petD 17 kDa polypeptide protein protein spiol Spinacia oleracea (Spinach) Spinach MGVTKKPDLNDPVLRAKLAKGMGHNYYGEPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPLEILPEWYFFPVFQILRTVPNKLLGVLLMASVPAGLLTVPFLENVNKFQNPFRRPVATTVFLVGTVVALWLGIGATLPIDKSLTLGLF 8FBDC1A34D4C2B07 peta-petb-5 1 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spiol Spinacia oleracea (Spinach) Spinach 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach physical association physical association Fig 1, Ext Tab 1 peta-petb-6 2 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spiol Spinacia oleracea (Spinach) Spinach 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spiol Spinacia oleracea (Spinach) Spinach physical association physical association Ext Fig 1a