DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. Science (New York, N.Y.) 2019 imex curation Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) saxs x ray scattering saxs experimental particp experimental participant identification experimental particp Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Accepted 2020-JAN-08 AT 11:12 GMT AT 11:12 GMT by ORCHARD Science (New York, N.Y.) 2019 imex curation NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. Science (New York, N.Y.) 2019 imex curation Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Accepted 2020-JAN-08 AT 11:12 GMT AT 11:12 GMT by ORCHARD Science (New York, N.Y.) 2019 imex curation NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. Science (New York, N.Y.) 2019 imex curation Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Horsefield S., Burdett H., Zhang X., Manik MK., Shi Y., Chen J., Qi T., Gilley J., Lai JS., Rank MX., Casey LW., Gu W., Ericsson DJ., Foley G., Hughes RO., Bosanac T., von Itzstein M., Rathjen JP., Nanson JD., Boden M., Dry IB., Williams SJ., Staskawicz BJ., Coleman MP., Ve T., Dodds PN., Kobe B. b.kobe@uq.edu.au Accepted 2020-JAN-08 AT 11:12 GMT AT 11:12 GMT by ORCHARD Science (New York, N.Y.) 2019 imex curation q6pds3-1 NAD(+) hydrolase SARM1 NADP(+) hydrolase SARM1 Sterile alpha and TIR motif-containing protein 1 Sarm1 Kiaa0524 protein protein mouse Mus musculus Mouse MVLTLLFSAYKLCRFFTMSGPRPGADRLTVPGPDRSGGASPWWAAGGRGSREVSPGVGTEVQGALERSLPELQQALSELKQASAARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALANCALHGGQTVQRCMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVEHSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQSLVLLLDSSRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTTSALAKRALRLLGEEVPRRILPCVASWKEAEVQTWLQQIGFSQYCENFREQQVDGDLLLRLTDEELQTDLGMKSSITRKRFFRELTELKTFASYATCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIRLGVHRTRILSAAREMLHSPLPCTGGKLSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVIAARNFVLVLSAGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQALPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRPSQDSSAGSDTSLEGATPMGLP 9A5BF03ED22E6102 q6szw1-1 NAD(+) hydrolase SARM1 SARM1 KIAA0524 SAMD2 SARM NADP(+) hydrolase SARM1 Sterile alpha and TIR motif-containing protein 1 Sterile alpha and Armadillo repeat protein Sterile alpha motif domain-containing protein 2 Tir-1 homolog protein protein human Homo sapiens Human MVLTLLLSAYKLCRFFAMSGPRPGAERLAVPGPDGGGGTGPWWAAGGRGPREVSPGAGTEVQDALERALPELQQALSALKQAGGARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALGNCALHGGQAVQRRMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVMGARNFVLVLSPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQVLPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRSSRDSSAGSDTSLEGAAPMGPT 6391EA4C31EF6604 sarm1-1 2 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 17:02:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu473Phe' Q6SZW1-1:p.[Leu446Met;Leu505Met;Leu523Met] L446M, L505M, L523M mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L M certain certain sequence position certain certain certain sequence position certain L M certain certain sequence position certain certain certain sequence position certain L M Wed Jan 29 12:07:52 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu446Met;Leu505Met;Leu523Met' Tue Jun 30 17:50:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu446Met;Leu505Met;Leu523Met]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 1B. Table S1 sarm1-4 1 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 17:44:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu473Phe' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig S11 sarm1-6 3 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q6SZW1-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain I D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain V D Wed Nov 13 14:26:24 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg; Ile461Asp; Leu514Asp; Leu531Asp; Val533Asp]' Wed Jan 29 11:21:53 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp' Tue Jun 30 17:49:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp]' Binding region Binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q6SZW1-1:p.Leu473Phe L473F mutation mutation certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 19:55:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu473Phe' His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3A, S11 sarm1-8 3 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q6PDS3-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain I D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain V D Wed Nov 13 12:51:06 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg; Ile461Asp; Leu514Asp; Leu531Asp; Val533Asp]' Wed Jan 29 12:54:01 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp' Tue Jun 30 17:45:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp]' ecoli Escherichia coli (strain K12) 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q6PDS3-1:p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp] mSARM1 tSAM(5Mut) mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain I D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain V D Wed Nov 13 12:51:06 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg; Ile461Asp; Leu514Asp; Leu531Asp; Val533Asp]' Wed Jan 29 12:10:15 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp' Tue Jun 30 16:37:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu442Arg;Ile461Asp;Leu514Asp;Leu531Asp;Val533Asp]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig S16C