IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy. DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy. Journal of cell science 2019 imex curation Nowak M., Suenkel B., Porras P., Migotti R., Schmidt F., Kny M., Zhu X., Wanker EE., Dittmar G., Fielitz J., Sommer T. 2016-04-04 submitted by M Nowak, Max Delbrueck Center, Berlin. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2019/nowak-2016 Bona fide interaction thresholds and additional construct details were provided by the authors upon email request. jens.fielitz@uni-greifswald.de,tsommer@mdc-berlin.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nowak M., Suenkel B., Porras P., Migotti R., Schmidt F., Kny M., Zhu X., Wanker EE., Dittmar G., Fielitz J., Sommer T. This experiment relates to a negative interaction. 2016-04-04 submitted by M Nowak, Max Delbrueck Center, Berlin. Files for this submission can be found in sp-pro6/intact/local/data/curation-material/2019/nowak-2016 Bona fide interaction thresholds and additional construct details were provided by the authors upon email request. jens.fielitz@uni-greifswald.de,tsommer@mdc-berlin.de Accepted 2019-SEP-16 AT 17:24 BST AT 17:24 BST by MGT Journal of cell science 2019 Only protein-protein interactions imex curation imex curation tri63_human E3 ubiquitin-protein ligase TRIM63 IRF Iris RING finger protein MURF1 Muscle-specific RING finger protein 1 RING finger protein 28 RNF28 SMRZ Striated muscle RING zinc finger protein Tripartite motif-containing protein 63 TRIM63 RING-type E3 ubiquitin transferase TRIM63 protein protein human Homo sapiens Human MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ 9BE4B1505039BC86 q13501-2 Sequestosome-1 SQSTM1 ORCA OSIL EBI3-associated protein of 60 kDa Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 protein protein human Homo sapiens Human MAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL Initiator Met-1 is removed. Contains a N-acetylalanine at position 2. trim63-sqstm1-1 1 Sqstm1/p62 Iso 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range MuRF1 2 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed C-terminal Myc-His6 tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association true 1C