DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. Science (New York, N.Y.) 2019 imex curation Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Accepted 2020-JUN-12 AT 15:50 BST AT 15:50 BST by PPORRAS Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. Science (New York, N.Y.) 2019 imex curation Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Accepted 2020-JUN-12 AT 15:50 BST AT 15:50 BST by PPORRAS Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation atg40_yeast Autophagy-related protein 40 ATG40 O3536 YOR152C protein protein yeast Saccharomyces cerevisiae Baker's yeast MFNLILWPLFLLTSVAIPLQLTLEVVYLTSSVDFSKASAAKTATSLGQSPVVITIYKSLLKYWSLYEFIHFIYLYTPIDAFLNFLPFTSLLMSFGSICLTRELVYDFIAFMESQNKLTGFLNKITEPNFNSYLLFSSIYNIWFADDTNDKFLFGKLTQILISVTKRYEFPRTFYLAKVSDFLQNLILTRLRPFVTEQPQGDKNRYQNGDRESTKNGAAYQKSSQQSSSFEQNFTSTEFPNDYDFMEDILDETTELD FD4951C817E2AD0F FD4951C817E2AD0F sfb3_yeast SED5-binding protein 3 SFB3 SEC24-related protein 3 Lethal with SEC13 protein 1 LST1 YHR098C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQQNILAASVSALSLDESTVHTGGASSKKSRRPHRAYHNFSSGTVPTLGNSPYTTPQLNQQDGFQQPQAFTPKQFGGFNNGSGSVMSTPVMVSQERFGASEASSPYGQSMLDMTAPQPTSHIVPTQRFEDQAQYLQRSFETCRDSVPPLPTTQFYCVDQGSCDPHLMSLSMYNIPESEHLRAATKLPLGLTIQPFSTLTPNDAEVPTIPLPMDGTPLRCRRCRAYANPKFQFTYDSSVICNICRVKMQVPGEHFAPMGPNGQRSDLNEKSELLHGTVDFLVPSIYNAIQEKELLPLHYVFLIDVSLLANENGSSLAMVEGVRSCIEYISDFQPNCEVAIIVYDNKLRFFNLRPDLDNAQEYIVSELDDVFLPFYNGLFVKPGNSMKIINDTLIKISGYISTDKYSHVPQVCYGSALQAAKLALDTVTGGQGGKIICSLNSLPTIGNGNLSLKRDNAHIAHVKCDNGFYKKLASDFLKSYISLDLYVTNAGFIDMATVGHPVEMTSGILKYYPHFQQETDAFTLVNDMVTNVSNIVGYQALLKVRCSTGLSVEQYYCDSSDNTDHDPIIPVLTRDTTLDVLLKYDSKIKTGTDVHFQTALLYTDIDGVRKVRSINTSGAVSNNIREIFKFINQNPVMRIMIKDVIKTLGDCDFVKIRRLIDDKMVEILTQYRGLVSSNSSTQLILPDSIKTLPAYMLAFEKSELMKPNAQSTRGNERIYDLLKYDSLNSAQLCYKLYPQIVPFHVLLEETDLTFYDANDKLLQINSSSINNLSVRASHSNFINGGCYLIFQGDTIYLWFNENTNRMLLQDLLSVDESLPVSQISLFSGTLPETGTSINQKASNVIKNWQQVVNKSSLPLVLLRPNVDQYYSNVMSQLLCEDKTVNRIESYDNYLVIMHKKIQEKLQKDDFIKVSTAATHENIHQKFVQF 6A028AD6B1A2E031 6A028AD6B1A2E031 atg40-1 1 3 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig 3A sfb3-atg40-1 2 3 tag visualisation tag visualisation experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level GFP Tag GFP Tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast 4 tag visualisation tag visualisation experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level mCherry Tag mCherry Tag mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast colocalization colocalization Fig 2A Rapamycin (CHEBI:9168)