DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein. The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein. Science (New York, N.Y.) 2019 imex curation Qi C., Sorrentino S., Medalia O., Korkhov VM. volodymyr.korkhov@psi.ch 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Qi C., Sorrentino S., Medalia O., Korkhov VM. volodymyr.korkhov@psi.ch Accepted 2020-APR-16 AT 08:25 BST AT 08:25 BST by LIVIAP Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein. The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein. Science (New York, N.Y.) 2019 imex curation Qi C., Sorrentino S., Medalia O., Korkhov VM. volodymyr.korkhov@psi.ch 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Qi C., Sorrentino S., Medalia O., Korkhov VM. volodymyr.korkhov@psi.ch Accepted 2020-APR-16 AT 08:25 BST AT 08:25 BST by LIVIAP Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation e1bm79_bovin ADCY9 protein protein bovin Bos taurus (Bovine) Bovine MASPPHQQLLQHHSTEVSCDSSGDSNSVRVRINPKQPSSNSHPKHCKYSISSSCSSSGDSGGVPRRMGAGGRLRRRKKLPQLFERASSRWWDPKFDSVNLEEACMERCFPQTQRRFRYALFYIGFACLLWSIYFGVHMKSKLIVMVAPALCFLVVCVGFFLFTFTKLYARHYVWTSLVLTLLVFALTLAAQFQVLTPLSGRVDNFNHTRAARPTDTCLSQVGSFSMCIEVLFLLYTVMHLPLYLSLILGVAYSVLFETFGYHFQDEACFASPGAEALHWELLSRALLHLCIHAIGIHLFIMSQVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEESENSVKRHATSSPKNRKKKSSIQKAPIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLNDLFGRFDRLCEETKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIRAIEQFCQEKKEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLDDRYEMEDGKVTERLGQSVVADQLKGLKTYLIAGQRAKESHCSCSEALLSGFEVLDGSRVSSGPRGQGTASPGSVSDLAQTVKTFDNLKTCPSCGITFTPKPEAGAEGGAVQNGCQEEPKNSAKASGGPSSKTQNGLLSPPPEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDPELERAYRTSYQEEVVKSSPVRTFASATFSSLLDVLLSTTVFLILSITCFLRYGAASTPPPPAALAVFGAALLLEILSLVVSVRMVFFLEDVMTCTKRLLEWIAGWLPRHFIGAILVSLPALAVYSHVTSEFETNIHSTMFTGSAVLTAVVQYCNFCQLSSWMRSSLATVVGAGPLLLLLYVSLCPDSSTVISHLDAVQNFSSTRKLCNASLPHDGRSPASLIGQEVILVFFLLLLLVWFLNREFEVSYRLHYHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQTYSKNHDSGGVIFASIVNFSEFYEENYEGGKECYRVLNELIGDFDELLSKPDYSSIEKIKTIGATYMAASGLNATQCRDGSHPQEHLQILFEFAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTTGVECRIQVSEESYRVLSKMGYEFDYRGTVNVKGKGQMKTYLYPKCTDSGLVPQHQLSISPDIRVQVDGSIGRSPTDEIASLVPSVQNPDQVPPGSENNAQTRDAHPSAKRPWKEPVRAEERCRFGKAIEKSDCEEVGMEEANELTKLNVSERA 331E053F0F3FC79F gnas2_bovin Guanine nucleotide-binding protein G(s) subunit alpha isoforms short GNAS GNAS1 Adenylate cyclase-stimulating G alpha protein protein protein bovin Bos taurus (Bovine) Bovine MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL DIP protein P04896 original sequence version: 127 DIP protein P04896-1 original sequence version: 164 4D07A734AFF2A6BC p04896-1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Adenylate cyclase-stimulating G alpha protein GNAS GNAS1 Alpha-S2 protein protein bovin Bos taurus (Bovine) Bovine MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL 4D07A734AFF2A6BC adcy9-gnas-1 2 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Strep tag Strap tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig 1, Table S1 adcy9-gnas-2 1 5 unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role neutral component neutral component purified purified YFP tag YFP tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Strep tag Strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig S1