DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature 2019 imex curation Ma Z., Zhu P., Shi H., Guo L., Zhang Q., Chen Y., Chen S., Zhang Z., Peng J., Chen J. pengjr@zju.edu.cn; chenjun2009@zju.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ma Z., Zhu P., Shi H., Guo L., Zhang Q., Chen Y., Chen S., Zhang Z., Peng J., Chen J. pengjr@zju.edu.cn; chenjun2009@zju.edu.cn Accepted 2019-JUL-15 AT 10:01 BST AT 10:01 BST by ORCHARD Nature 2019 imex curation PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature 2019 imex curation Ma Z., Zhu P., Shi H., Guo L., Zhang Q., Chen Y., Chen S., Zhang Z., Peng J., Chen J. pengjr@zju.edu.cn; chenjun2009@zju.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Ma Z., Zhu P., Shi H., Guo L., Zhang Q., Chen Y., Chen S., Zhang Z., Peng J., Chen J. pengjr@zju.edu.cn; chenjun2009@zju.edu.cn Accepted 2019-JUL-15 AT 10:01 BST AT 10:01 BST by ORCHARD Nature 2019 imex curation ren3a_danre Regulator of nonsense transcripts 3A upf3a Up-frameshift suppressor 3 homolog A si:dkey-21o13.6 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MRSEKEQRTGSRERGSVEIQFRDCQDNAAVNPKHKEEKKEIFSKVVIRRLPPSLSKDQLQEHLSPLPSFDYFEFFPADQSLYPHLFSRAYINFKNPEDIIIFRDRFDGYVFIDNKGQEYPAVVEFAPFQKVSKKKLKKKDAKAGTIEEDPEYRRFLENYSCDEEKSMANPETLLGEIEAKTRELIAKRTTPLLEYIKNKKLEKQRIREEKREERRRRELEKKRQREEEKRKRREEERQKRKEAEKQKKLSEKEIKIKLLKKCDRDDDVDSDRLKDKGDSGETEKNRWEKPGGHTKSKDSKDNRSQMENDKEQREGHGRRQRDKDHRGRDEERKRQRHHYEFDKFMRRKEETKWGKGYCQDRAKKDQHHGYSYCPETGDKLGKEDREDMGSRKERIRNKDRPAMQLYQPGARNRKRMGSGNKTFDFPPISPEHAGEHCYKTVIGTGSEKSADE 14118036565D09D8 f1qst7_danre rbbp5 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLSWSRDGHKLVSASTDNIVSQWDVLTGDCDQRFRFPSPILKLQYHPRDMNKVLVCPMKSAPVLLTLSDSKHVVLPVDDDSDLNVVAAFDRRGEYIYTGNAKGKILVLNTDTQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKRCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIPAFCSSDEELEDYKALLYLPIAPEVEDPEENPFGPPPDATAQNASTDDGSTHTGPGDKKRHPSADGAPPKKKIRTTTIELQGVPSDEVHPLLGVKGDSKSKKKAAGRPKGSKGNLVAAVYKQHEVQGLD 64CB0403A4E8AF13 q7ztx2_danre wdr5 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MATEEKKPDTEATKTQPASAASASQSKSAPVKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC 2E6738624C4CEB63 rbbp5-upf3a-1 2 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 3 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human Homo sapiens Human association association Fig. 3g and Supplementary Figure 1d rbbp5-wdr5-6 2 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association Fig. 3g, 3h wdr5-upf3a-1 2 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 3 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human Homo sapiens Human physical association physical association Supplementary Figure 1a and 1c wdr5-upf3a-2 2 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 3 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association Supplementary Figure 1b wdr5-rbbp5-2 1 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed purified purified ha tag ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed purified purified GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1f wdr5-upf3a-3 1 3 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed purified purified GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1f wdr5-rbbp5-1 1 3 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed purified purified HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed purified purified GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association Supplementary Figure 1g