MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. The FEBS journal 2019 imex curation Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it Accepted 2019-SEP-23 AT 13:40 BST AT 13:40 BST by LUANA The FEBS journal 2019 Only protein-protein interactions imex curation imex curation Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. The FEBS journal 2019 imex curation Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it Accepted 2019-SEP-23 AT 13:40 BST AT 13:40 BST by LUANA The FEBS journal 2019 Only protein-protein interactions imex curation imex curation Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. The FEBS journal 2019 imex curation Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro phage display phage display nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it Accepted 2019-SEP-23 AT 13:40 BST AT 13:40 BST by LUANA The FEBS journal 2019 Only protein-protein interactions imex curation imex curation Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. The FEBS journal 2019 imex curation Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it Accepted 2019-SEP-23 AT 13:40 BST AT 13:40 BST by LUANA The FEBS journal 2019 Only protein-protein interactions imex curation imex curation Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8. The FEBS journal 2019 imex curation Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Castagnoli L., Mandaliti W., Nepravishta R., Valentini E., Mattioni A., Procopio R., Iannuccelli M., Polo S., Paci M., Cesareni G., Santonico E. interaction revealed with P4D1 antibody in figs 5e and 7c were not annotated because the antibody cross-reacts with ubiq and nedd8. Elena.Santonico@uniroma2.it; Cesareni@uniroma2.it Accepted 2019-SEP-23 AT 13:40 BST AT 13:40 BST by LUANA The FEBS journal 2019 Only protein-protein interactions imex curation imex curation ubp13_human Ubiquitin carboxyl-terminal hydrolase 13 Ubiquitin-specific-processing protease 13 Deubiquitinating enzyme 13 Isopeptidase T-3 USP13 ISOT3 Ubiquitin thioesterase 13 protein protein human Homo sapiens Human MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS DIP protein Q92995 original sequence version: 132 988713ADF8C0B938 cblb_human E3 ubiquitin-protein ligase CBL-B Signal transduction protein CBL-B SH3-binding protein CBL-B Casitas B-lineage lymphoma proto-oncogene b RING finger protein 56 CBLB RNF56 Nbla00127 RING-type E3 ubiquitin transferase CBL-B protein protein human Homo sapiens Human MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEPIIVDPFDPRDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPPERPPPIPPDNRLSRHIHHVESVPSRDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSNVNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPLANSLSEKTRDPVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPDLSIYLKGDVFDSASDPVPLPPARPPTRDNPKHGSSLNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRLNL B4E307D31B9F0779 sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD poli_human DNA polymerase iota Eta2 RAD30 homolog B POLI RAD30B protein protein human Homo sapiens Human MEKLGVEPEEEGGGDDDEEDAEAWAMELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQKLGTGNYDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKALNTAKKGLIDYYLMPSLSTTSRSGKHSFKMKDTHMEDFPKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDINEFPLCSLPEGVDQEVFKQLPVDIQEEILSGKSREKFQGKGSVSCPLHASRGVLSFFSKKQMQDIPINPRDHLSSSKQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYLDNRLKDERISQGPKEPQGFHFTNSNPAVSAFHSFPNLQSEQLFSRNHTTDSHKQTVATDSHEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKELLAEWKRAGSDFHIGHK C1A5BF0894E91FDF ubql1_human Ubiquilin-1 Protein linking IAP with cytoskeleton 1 UBQLN1 DA41 PLIC1 protein protein human Homo sapiens Human MAESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS 8B4756B6113B7025 n4bp1_human NEDD4-binding protein 1 N4BP1 KIAA0615 protein protein human Homo sapiens Human MAARAVLDEFTAPAEKAELLEQSRGRIEGLFGVSLAVLGALGAEEPLPARIWLQLCGAQEAVHSAKEYIKGICEPELEERECYPKDMHCIFVGAESLFLKSLIQDTCADLCILDIGLLGIRGSAEAVVMARSHIQQFVKLFENKENLPSSQKESEVKREFKQFVEAHADNYTMDLLILPTSLKKELLTLTQGEENLFETGDDEVIEMRDSQQTEFTQNAATGLNISRDETVLQEEARNKAGTPVSELTKQMDTVLSSSPDVLFDPINGLTPDEEALSNERICQKRRFSDSEERHTKKQFSLENVQEGEILHDAKTLAGNVIADLSDSSADSENLSPDIKETTEEMEYNILVNFFKTMGYSQEIVEKVIKVYGPSTEPLLLLEEIEKENKRFQEDREFSAGTVYPETNKTKNKGVYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCRINTFRTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSRGASSHQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCSTLSPPMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELKNEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITVFVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLLHLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDPLGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSHQPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELREALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD DIP protein O75113 original sequence version: 93 171EDCC9D3BCF3D3 khnyn_human Protein KHNYN KHNYN KH and NYN domain-containing protein KIAA0323 protein protein human Homo sapiens Human MPTWGARPASPDRFAVSAEAENKVREQQPHVERIFSVGVSVLPKDCPDNPHIWLQLEGPKENASRAKEYLKGLCSPELQDEIHYPPKLHCIFLGAQGFFLDCLAWSTSAHLVPRAPGSLMISGLTEAFVMAQSRVEELAERLSWDFTPGPSSGASQCTGVLRDFSALLQSPGDAHREALLQLPLAVQEELLSLVQEASSGQGPGALASWEGRSSALLGAQCQGVRAPPSDGRESLDTGSMGPGDCRGARGDTYAVEKEGGKQGGPREMDWGWKELPGEEAWEREVALRPQSVGGGARESAPLKGKALGKEEIALGGGGFCVHREPPGAHGSCHRAAQSRGASLLQRLHNGNASPPRVPSPPPAPEPPWHCGDRGDCGDRGDVGDRGDKQQGMARGRGPQWKRGARGGNLVTGTQRFKEALQDPFTLCLANVPGQPDLRHIVIDGSNVAMVHGLQHYFSSRGIAIAVQYFWDRGHRDITVFVPQWRFSKDAKVRESHFLQKLYSLSLLSLTPSRVMDGKRISSYDDRFMVKLAEETDGIIVSNDQFRDLAEESEKWMAIIRERLLPFTFVGNLFMVPDDPLGRNGPTLDEFLKKPARTQGSSKAQHPSRGFAEHGKQQQGREEEKGSGGIRKTRETERLRRQLLEVFWGQDHKVDFILQREPYCRDINQLSEALLSLNF 4E52D096B545430D cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA DIP protein Q13616 original sequence version: 144 DIP protein Q13616 original sequence version: 133 DIP protein Q13616 original sequence version: null DIP protein Q13616 original sequence version: 135 DIP protein Q13616 original sequence version: 98 DIP protein Q13616 original sequence version: 110 DIP protein Q13616 original sequence version: 146 DIP protein Q13616 original sequence version: 148 DIP protein Q13616 original sequence version: 100 DIP protein Q13616 original sequence version: 85 DIP protein Q13616 original sequence version: 114 DIP protein Q13616 original sequence version: 119 DIP protein Q13616 original sequence version: 136 DIP protein Q13616 original sequence version: 117 DIP protein Q13616 original sequence version: 99 DIP protein Q13616 original sequence version: 108 6625A1FFA7799BBA psmd4_human 26S proteasome non-ATPase regulatory subunit 4 PSMD4 MCB1 26S proteasome regulatory subunit S5A 26S proteasome regulatory subunit RPN10 Multiubiquitin chain-binding protein Antisecretory factor 1 protein protein human Homo sapiens Human MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK DIP protein P55036 original sequence version: 149 DIP protein P55036 original sequence version: 139 EC712EC4DB1CE9AB ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rn114_human E3 ubiquitin-protein ligase RNF114 Zinc finger protein 313 RNF114 ZNF313 ZNF228 Zinc finger protein 228 RING finger protein 114 RING-type E3 ubiquitin transferase RNF114 protein protein human Homo sapiens Human MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMNQVLQRSIIDQ DF5A962B1EC13A21 cul2_human Cullin-2 CUL2 protein protein human Homo sapiens Human MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA DIP protein Q13617 original sequence version: 129 DIP protein Q13617 original sequence version: 127 DIP protein Q13617-1 original sequence version: 139 DIP protein Q13617 original sequence version: 111 DIP protein Q13617 original sequence version: 102 30647248F671AB0E yaf2_human YY1-associated factor 2 YAF2 protein protein human Homo sapiens Human MGDKKSPTRPKRQPKPSSDEGYWDCSVCTFRNSAEAFKCMMCDVRKGTSTRKPRPVSQLVAQQVTQQFVPPTQSKKEKKDKVEKEKSEKETTSKKNSHKKTRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKTKSPPASSAASADQHSQSGSSSDNTERGMSRSSSPRGEASSLNGESH 199A56EE06BB4FBE ubl7_human Ubiquitin-like protein 7 Ubiquitin-like protein SB132 Bone marrow stromal cell ubiquitin-like protein UBL7 BMSCUBP SB132 protein protein human Homo sapiens Human MSLSDWHLAVKLADQPLTPKSILRLPETELGEYSLGGYSISFLKQLIAGKLQESVPDPELIDLIYCGRKLKDDQTLDFYGIQPGSTVHVLRKSWPEPDQKPEPVDKVAAMREFRVLHTALHSSSSYREAVFKMLSNKESLDQIIVATPGLSSDPIALGVLQDKDLFSVFADPNMLDTLVPAHPALVNAIVLVLHSVAGSAPMPGTDSSSRSMPSSSYRDMPGGFLFEGLSDDEDDFHPNTRSTPSSSTPSSRPASLGYSGAAGPRPITQSELATALALASTPESSSHTPTPGTQGHSSGTSPMSSGVQSGTPITNDLFSQALQHALQASGQPSLQSQWQPQLQQLRDMGIQDDELSLRALQATGGDIQAALELIFAGGAP 0B64DF79597E0931 nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ DIP protein Q15843 original sequence version: 103 DIP protein Q15843 original sequence version: 149 DIP protein Q15843 original sequence version: 58 DIP protein Q15843 original sequence version: 124 DIP protein Q15843 original sequence version: 95 DIP protein Q15843 original sequence version: 111 DC2FE102BE4725D2 otud5_human OTU domain-containing protein 5 Deubiquitinating enzyme A OTUD5 protein protein human Homo sapiens Human MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRDPPPDKS F4B2B385B84ABC46 taxb1_human Tax1-binding protein 1 TRAF6-binding protein TAX1BP1 T6BP PRO0105 protein protein human Homo sapiens Human MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHPHPKDWVGIFKVGWSTARDYYTFLWSPMPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQFCYVTHKGEIRGASTPFQFRASSPVEELLTMEDEGNSDMLVVTTKAGLLELKIEKTMKEKEELLKLIAVLEKETAQLREQVGRMERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKKRFSDATSKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKELYKVHLKNTEIENTKLMSEVQTLKNLDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTSSKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVKKQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQKQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFDIVTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENVKLELAEVQDNYKELKRSLENPAERKMEGQNSQSPQCFKTCSEQNGYVLTLSNAQPVLQYGNPYASQETRDGADGAFYPDEIQRPPVRVPSWGLEDNVVCSQPARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCELMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD DE8460DAA6712125 rd23a_human UV excision repair protein RAD23 homolog A RAD23A protein protein human Homo sapiens Human MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE DIP protein P54725 original sequence version: 141 DIP protein P54725 original sequence version: 136 DIP protein P54725 original sequence version: 139 C4E47E9313BB47B5 rad23a-ubc-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 nedd8-rad23a-1 3 23 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association table 1 fig.1 nedd8-poli-1 3 9 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association table 1 fig.1 nedd8-ubqln1-1 3 10 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association table 1 fig.1 nedd8-khnyn-1 3 12 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association table 1 fig.1 nedd8-cblb-1 3 7 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association table 1 fig.1 ubiq_human-poli-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-ubqln1-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-khnyn-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-cblb-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-usp13-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-sqstm1-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-ubl7-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 19 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-n4bp1-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-tax1bp1-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 22 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-rnf114-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-yaf2-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-otud5-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 21 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 ubiq_human-psmd4-1 3 15 predetermined predetermined participant predetermined nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey phage library phage library region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lambd Enterobacteria phage lambda Bacteriophage lambda physical association physical association table 1 fig.1 nedd8-n4bp1-1 4 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ala72Arg mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 10:57:51 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 17:56:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b nedd8-rad23a-2 4 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ile44Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 12:09:51 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 19:46:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:29:51 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 18:51:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b nedd8-poli-2 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ile44Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 11:52:57 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 18:23:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A R Tue Jun 30 17:39:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b nedd8-yaf2-1 4 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ile44Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 11:57:41 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 16:58:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:49:26 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 18:57:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b nedd8-ubl7-1 4 19 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ile44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 11:35:14 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 16:39:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:35:05 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 19:11:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b nedd8-khnyn-2 4 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Ile44Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 11:38:31 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 15:56:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:20:37 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 17:30:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) physical association physical association fig 2b otud5-ubiq_human-1 4 21 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-rnf114-2 4 16 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-ubl7-2 4 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-ubl7-3 4 19 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-n4bp1-2 4 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-rad23a-1 4 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b ubiq_human-poli-2 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b khnyn-nedd8-1 4 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 12 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association fig 3c, fig 5b T‐Rex‐flag‐NEDD8 cell lysates were therefore incubated with the purified proteins bound to glutathione‐Sepharose beads and affinity‐purified proteins were analysed by western blotting with anti‐flag antibody (Fig. 3C). As observed, the purified UBDs considerably differ in their ability to bind neddylated substrates, KHNYN being the one displaying the highest binding efficiency. In addition, the UBD of KHNYN was the only one able to bind both neddylated substrates and unconjugated flag‐NEDD8, as detected by longer exposure. To further confirm that the molecular determinants governing NEDD8 recognition are found in the C‐terminal 52 residues of KHNYN, we generated two chimeras by swapping the amino acid regions corresponding to the three α‐helices. The first chimeric construct, KN, contains the N‐terminal unique region of KHNYN (aa 598–634) and the α‐helices of N4BP1 (aa 854–896), while in the NK construct the last three helices of KHNYN (aa 635–678) are fused to the N‐terminal region of N4BP1 (aa 813–852) (Fig. 5C). The purified recombinant proteins were analysed by pull‐down assays for the ability to bind neddylated substrates in the T‐Rex‐flag‐NEDD8 cells. As shown, the chimeric construct NK retains the properties of wild‐type KHNYN, as it is able to bind to conjugated and monomeric NEDD8. khnyn-nedd8-2 4 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association fig 6a ubiq_human-khnyn-2 4 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association fig 6a khnyn-nedd8-3 2 20 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association fig 6b As shown in Fig. 6A, analogously to the isolated CUBAN domain, the full‐length protein shows a marked preference for NEDD8. The essential role of the CUBAN domain in the recognition of NEDD8 and neddylated substrates was also confirmed by coimmunoprecipitation in HeLa cells transiently transfected with flag‐KHNYN (Fig. 6B). khnyn-nedd8-4 4 13 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 17 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 12 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association fig 6c As shown in Fig. 6C, several neddylated substrates were affinity purified by the GST fusion of the KHNYN ubiquitin‐binding domain, among them CUL1 and CUL2, both of which bound to KHNYN in their neddylated forms. Conversely, no significant association was observed with the other constructs. Similar results were obtained for CUL3 and CUL4 (data not shown). cul2-nedd8-1 4 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 12 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 flp-in Homo sapiens 293 flp-in 293 flp-in association association fig 6e MLN4924 inhibitor of NEDD8 activation khnyn-cul2-1 4 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293 flp-in Homo sapiens 293 flp-in 293 flp-in 12 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 flp-in Homo sapiens 293 flp-in 293 flp-in association association fig 6f f5d f5c . Finally, we compared the interaction of KHNYN with ubiquitinated and neddylated substrates after treatment with USP8, the deneddylating enzyme NEDP1 or MLN4924 (Fig. 6F). As shown, the interaction with flag‐tagged cellular proteins was similarly disrupted after MLN4924 or NEDP1 treatments (Fig. 6F lanes 6 and 7). Interestingly, the interaction with ubiquitinated substrates was partly affected by the removal of neddylated proteins (lane 7), suggesting that a fraction of ubiquitinated substrates recovered by pull‐down are associated with cullin‐based complexes. khnyn-nedd8-5 1 20 unspecified role unspecified role neutral component neutral component purified purified Q15843:p.Ala72Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:45:01 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 18:12:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 7b ITC titration of NEDD8 with the KHNYN domain shows an exothermic binding thermogram (Fig. 7B). The uncertainty in the early steps of the exponential curve allows only rough indications of the binding affinity of the CUBAN domain of KHNYN in complex with NEDD8 that was evaluated to be around 24 ± 2 μm. Finally, NEDD8 A72R mutation causes a reduction in the interaction efficiency (44 ± 7 μm) while binding of KHNYN for ubiquitin was nearly undetectable in these conditions. khnyn-nedd8-6 5 20 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 9; fig 10; fig 12 nedd8-khnyn-3 4 20 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15843:p.Glu31Gln mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E Q Tue Mar 19 11:28:28 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.glu31gln' Tue Jun 30 15:43:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu31Gln' Q15843:p.Ile44Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A Tue Mar 19 11:08:36 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ile44ala' Tue Jun 30 16:06:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile44Ala' Q15843:p.Ala72Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A R Tue Mar 19 11:02:39 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ala72arg' Tue Jun 30 19:55:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' Q15843:p.Glu31_Glu32delinsGlnAsp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain EE QD Tue Mar 19 11:05:43 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.glu_glu31-32gln_asp' Tue Jun 30 20:13:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu31_Glu32delinsGlnAsp' Q15843:p.Glu31Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E D Tue Mar 19 11:12:49 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.glu31asp' Tue Jun 30 17:03:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu31Asp' Q15843:p.Glu31Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E K Tue Mar 19 12:00:54 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.glu31lys' Tue Jun 30 18:00:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu31Lys' Q15843:p.Glu32Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E K Tue Mar 19 11:51:02 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.glu32lys' Tue Jun 30 16:35:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu32Lys' Q15843:p.Glu31_Glu32delinsLysLys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain EE KK Tue Mar 19 11:08:00 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p-glu_glu31-32lys_lys' Tue Jun 30 15:38:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu31_Glu32delinsLysLys' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 11b To confirm our model, we mutated residues Glu31 and Glu32 in NEDD8 by substituting them with the corresponding residues in ubiquitin (Glu31Gln and Glu32Asp) or with an opposite charge (Glu31Lys and Glu32Lys). These mutants, together with the Ile44Ala and the Ala72Arg mutants, were assayed in a pull‐down experiment against the purified CUBAN domain (Fig. 11B). As shown, there is a clear loss of binding when mutating the hydrophobic patch key residue Ile44, while the Ala72Arg mutation reduces the binding efficiency as previously shown. In addition, there is a reduction in binding efficiency when using the mutants H25F and R38E, but not K26D and R33E, in the electrostatic binding surface of CUBAN domain (Fig. 11C). khnyn-nedd8-7 4 20 unspecified role unspecified role unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified O15037:p.Trp647Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W P Tue Mar 19 11:14:13 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.trp647pro' Tue Jun 30 19:14:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp647Pro' gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O15037:p.Trp647Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W A Tue Mar 19 12:00:18 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.trp647ala' Tue Jun 30 17:41:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp647Ala' O15037:p.His651Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H F Tue Mar 19 12:07:43 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.his651phe' Tue Jun 30 18:57:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His651Phe' O15037:p.Lys652Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K D Tue Mar 19 12:08:30 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.lys652asp' Tue Jun 30 18:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys652Asp' O15037:p.Arg659Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R E Tue Mar 19 11:43:05 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.arg659glu' Tue Jun 30 19:38:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg659Glu' O15037:p.Arg664Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R E Tue Mar 19 11:33:20 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.arg664glu' Tue Jun 30 16:45:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg664Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 11c ubiq_human-sqstm1-2 4 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.ile44ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I A ecoli Escherichia coli (strain K12) physical association physical association fig 2b khnyn-nedd8-8 4 20 unspecified role unspecified role prey prey purified purified Q15843:p.Ala72Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A R Tue Jun 30 19:08:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala72Arg' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction fig 7c