UniProt UniProt http://www.uniprot.org Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Although it says HSP:FKBP ratio 1:1 in the figure, the text makes it clear the they are regarding the HSP dimer as 1 Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Although it says HSP:FKBP ratio 1:1 in the figure, the text makes it clear the they are regarding the HSP dimer as 1 Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. For affinity calculations IANBD amide (N,N′-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine fluorescent dye was measured in the presence of increasing concentrations of binding protein. Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Source of AHSA1 taken from work by the same author (PPR:PPR70419) Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Cross linker:BS3 (bis(sulfosuccinimidyl) suberate. Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. For affinity calculations IANBD amide (N,N′-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine fluorescent dye was measured in the presence of increasing concentrations of binding protein. Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex. Nature communications 2018 imex curation Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Although it says HSP:FKBP ratio 1:1 in the figure, the text makes it clear the they are regarding the HSP dimer as 1 Markus.Zweckstetter@dzne.de Accepted 2019-SEP-18 AT 09:07 BST AT 09:07 BST by MGT Nature communications 2018 Only protein-protein interactions imex curation imex curation fkbp5_human Peptidyl-prolyl cis-trans isomerase FKBP5 FKBP5 AIG6 FKBP51 FK506-binding protein 5 51 kDa FK506-binding protein Rotamase 54 kDa progesterone receptor-associated immunophilin FKBP54 FF1 antigen HSP90-binding immunophilin Androgen-regulated protein 6 protein protein human Homo sapiens Human MTTDEGAKNNEESPTATVAEQGEDITSKKDRGVLKIVKRVGNGEETPMIGDKVYVHYKGKLSNGKKFDSSHDRNEPFVFSLGKGQVIKAWDIGVATMKKGEICHLLCKPEYAYGSAGSLPKIPSNATLFFEIELLDFKGEDLFEDGGIIRRTKRKGEGYSNPNEGATVEIHLEGRCGGRMFDCRDVAFTVGEGEDHDIPIGIDKALEKMQREEQCILYLGPRYGFGEAGKPKFGIEPNAELIYEVTLKSFEKAKESWEMDTKEKLEQAAIVKEKGTVYFKGGKYMQAVIQYGKIVSWLEMEYGLSEKESKASESFLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDFEKVLEVNPQNKAARLQISMCQKKAKEHNERDRRIYANMFKKFAEQDAKEEANKAMGKKTSEGVTNEKGTDSQAMEEEKPEGHV DIP protein Q13451 original sequence version: 109 18A86608C6891A73 p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636-8 original sequence version: 206 DIP protein P10636-8 original sequence version: 203 DIP protein P10636-8 original sequence version: 166 DIP protein P10636-8 original sequence version: 218 835A8706D847A8CC amp-pnp AMP-PNP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hs90b_human Heat shock protein HSP 90-beta HSP90AB1 HSP90B HSPC2 HSPCB Heat shock 84 kDa protein protein human Homo sapiens Human MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD DIP protein P08238 original sequence version: 91 DIP protein P08238 original sequence version: 149 DIP protein P08238 original sequence version: 165 DIP protein P08238 original sequence version: 167 DIP protein P08238 original sequence version: 210 DIP protein P08238 original sequence version: 187 A93118C214D03810 atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ahsa1_human Activator of 90 kDa heat shock protein ATPase homolog 1 HSPC322 p38 AHSA1 C14orf3 protein protein human Homo sapiens Human MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF E6B686DDD8D7D729 hsp90ab1-4 3 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B, Supp. Fig. 1B adp-hsp90ab1-1 3 13 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-3 3 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-4 3 13 unspecified role unspecified role unspecified role unspecified role 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-5 3 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B, Supp. Fig. 1B adp-hsp90ab1-2 3 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1B hsp90ab1-mapt-4 3 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1B adp-fkbp5-1 3 13 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1B amp_pnp-fkbp5-1 3 12 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1B amp_pnp-fkbp5-2 3 12 unspecified role unspecified role unspecified role unspecified role 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 1B atp-hsp90ab1-1 5 15 enzyme target enzyme target substrate substrate neutral component neutral component 14 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Fig. 1C ahsa1-atp-1 5 16 enzyme regulator enzyme regulator unspecified role unspecified role purified purified n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 15 enzyme target enzyme target substrate substrate neutral component neutral component 14 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Fig. 1C AHSA1 enhances HSP90AB1 ATPase activity atp-fkbp5-1 5 10 enzyme regulator enzyme regulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 15 enzyme target enzyme target substrate substrate neutral component neutral component atpase reaction atpase reaction Fig. 1C Full length FKBP5, but not the intrinsically disordered MAPT decreased the rate of HSP90AB1 ATP hydrolysis fkbp5-hsp90ab1-6 2 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1D, Supp Fig.1C-D, 2C-E, 3A fkbp5-hsp90ab1-7 4 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 ancillary ancillary neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain M C Tue Jun 30 19:51:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met363Cys' cys-363 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 4A HSP90AB1 is pre-bound to MAPT. hsp90ab1-mapt-5 4 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain M C Tue Jun 30 19:55:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met363Cys' cys-363 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4A fkbp5-hsp90ab1-8 1 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 4C, Supplementary Figure 8. fkbp5-hsp90ab1-9 2 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 4D, Supp. Fig 6C hsp90ab1-mapt-6 2 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4D, 4H Phosphorylation of MAPT by the microtubule affinity-regulating kinase and insertion of glutamic acid at specific phosphorylation epitopes abolished/weakened MAPTs interaction with HSP90AB1 and FKBP5. fkbp5-mapt-1 2 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4D,4H, Supp Fig. 6B Phosphorylation of MAPT by the microtubule affinity-regulating kinase and insertion of glutamic acid at specific phosphorylation epitopes abolished/weakened MAPTs interaction with HSP90AB1 and FKBP5. fkbp5-hsp90ab1-10 6 10 unspecified role unspecified role neutral component neutral component purified purified lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-8 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-38 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-415 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-230 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-400 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-402 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-406 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-491 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-551 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-557 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-577 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-623 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-685 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 143 163 162 144 164 145 165 146 147 166 148 167 149 168 169 150 151 171 152 172 153 173 174 154 155 175 176 156 157 177 170 158 direct interaction direct interaction Supp. Fig. 3B, Supplementary Table 2 hsp90ab1-mapt-7 6 11 unspecified role unspecified role neutral component neutral component purified purified lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-353 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-257 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-298 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-267 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-267 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-294 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-2 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-163 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-180 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-44 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-240 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-148 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-234 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-291 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-163 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-481 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-552 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-573 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-623 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-649 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-649 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range lys-64 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-180 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-186 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-199 125i radiolabel 125i radiolabel I125 125I 125I I125 certain certain sequence position certain certain certain sequence position certain lys-204 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-219 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-284 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-347 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-347 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-481 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 180 221 181 212 182 218 213 183 184 243 185 215 186 216 217 187 219 188 220 189 222 190 191 223 192 226 193 227 194 228 195 229 196 230 197 231 232 198 199 233 200 234 201 235 202 236 203 237 238 204 205 239 206 240 241 207 208 242 209 214 210 244 direct interaction direct interaction Supp. Fig. 3b, Supp. Table 3 fkbp5-hsp90ab1-11 6 11 unspecified role unspecified role neutral component neutral component purified purified lys-375 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-281 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-257 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-369 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-317 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-140 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-383 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified lys-352 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-272 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-28 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-58 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-153 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-272 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-280 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 247 278 248 268 269 249 271 250 272 251 252 286 253 273 274 254 255 275 256 276 257 277 280 258 281 259 260 282 261 283 262 284 263 285 264 287 265 279 270 266 physical association physical association Fig. 3B, Supplementary Table 4 fkbp5-hsp90ab1-12 2 11 unspecified role unspecified role neutral component neutral component purified purified mstl tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Supp Table 5, Supp. Fig. 7A hsp90ab1-mapt-8 2 11 unspecified role unspecified role neutral component neutral component purified purified mstl tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Supp Table 5, SUpp. Fig. 7A hsp90ab1-9 9 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1A fkbp5-hsp90ab1-13 2 10 unspecified role unspecified role neutral component neutral component purified purified region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2B-D fkbp5-hsp90ab1-15 8 11 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig. 6A HSP90AB1 is pre-bound to MAPT. fkbp5-hsp90ab1-16 7 10 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig 5 B/C fkbp5-fkbp5-1 4 10 unspecified role unspecified role neutral component neutral component purified purified tpr repeat region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain M C Tue Jun 30 16:53:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met363Cys' cys-363 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2H