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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="29459785" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-21451445" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-27364" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2018</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Yoo J., Mashalidis EH., Kuk ACY., Yamamoto K., Kaeser B., Ichikawa S., Lee SY.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">seok-yong.lee@duke.edu</attribute>
            <attribute name="author-announcement">21-Nov-2019: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="29459785" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-27364" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="7108">
            <names>
              <shortLabel>spofr-sf_9</shortLabel>
              <fullName>Spodoptera frugiperda insect cells</fullName>
              <alias type="synonym" typeAc="MI:1041">Fall armyworm</alias>
            </names>
            <cellType>
              <names>
                <shortLabel>sf_9</shortLabel>
                <fullName>insect cells</fullName>
              </names>
              <xref>
                <primaryRef db="cabri" dbAc="MI:0246" id="ACC 125" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="IA:0078" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="mint" dbAc="MI:0471" id="MINT-86941" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-346407" refType="identity" refTypeAc="MI:0356"/>
              </xref>
              <attributeList>
                <attribute name="comment" nameAc="MI:0612">Derived from pupal ovarian tissue of the fall armyworm</attribute>
                <attribute name="comment" nameAc="MI:0612">Cloned from the parent cell line, IPLB-SF-21-AE (SF-21)</attribute>
                <attribute name="comment" nameAc="MI:0612">derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae</attribute>
                <attribute name="comment" nameAc="MI:0612">cells are susceptible to Baculovirus infection</attribute>
              </attributeList>
            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Yoo J., Mashalidis EH., Kuk ACY., Yamamoto K., Kaeser B., Ichikawa S., Lee SY.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">seok-yong.lee@duke.edu</attribute>
          <attribute name="accepted">Accepted 2019-SEP-30 AT 13:10 BST AT 13:10 BST by MARTA</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2018</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>gpt_human</shortLabel>
          <fullName>UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
          <alias type="gene name" typeAc="MI:0301">DPAGT1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">DPAGT2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">GlcNAc-1-P transferase</alias>
          <alias type="gene name synonym" typeAc="MI:0302">N-acetylglucosamine-1-phosphate transferase</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9H3H5" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="O15216" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q9BWE6" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q86WV9" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000346142" version="SP_186" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000386597" version="SP_186" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3922860" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001373.2"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_016872782.1"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000172269" version="SP_186" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000354202" version="SP_186" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000409993" version="SP_186" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003975" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003976" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005789" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006047" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006487" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006488" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006489" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008963" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016020" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016757" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019348" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030176" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043231" version="SP_186"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0046872" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6BW5" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6BW6" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6FM9" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6FWZ" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6JQ3" version="SP_186"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-446193" version="SP_186"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-4549356" version="SP_186"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000715" version="SP_186"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR033895" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5LEV" version="SP_186"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5O5E" version="SP_186"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRPILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVRLFYDV</sequence>
        <attributeList>
          <attribute name="crc64">0AE10EFE55E7B9E0</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-27364-1">
        <names>
          <shortLabel>dpagt1-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="6BW5" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="6BW6" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-21451462" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-27364-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>structure determination</shortLabel>
                  <fullName>identification by structure determination</fullName>
                  <alias type="synonym" typeAc="MI:1041">identification by structure determination</alias>
                  <alias type="synonym" typeAc="MI:1041">structure determination</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2287" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-20597534" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="7877166" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="-1">
                <names>
                  <shortLabel>in vitro</shortLabel>
                  <fullName>In vitro</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Fig 1, Table 1</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>