UniProt UniProt http://www.uniprot.org Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. Interactions involving the TNRC6A construct have not been added as the origin of this construct was not given t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins. Cell reports 2017 imex curation Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bridge KS., Shah KM., Li Y., Foxler DE., Wong SCK., Miller DC., Davidson KM., Foster JG., Rose R., Hodgkinson MR., Ribeiro PS., Aboobaker AA., Yashiro K., Wang X., Graves PR., Plevin MJ., Lagos D., Sharp TV. Have annotated m-Tan as an RFP as I cannot find anything about it. t.sharp@qmul.ac.uk Accepted 2019-OCT-21 AT 09:45 BST AT 09:45 BST by MGT Cell reports 2017 Only protein-protein interactions imex curation imex curation tnr6a_human Trinucleotide repeat-containing gene 6A protein TNRC6A CAGH26 KIAA1460 TNRC6 CAG repeat protein 26 Glycine-tryptophan protein of 182 kDa GW182 autoantigen EMSY interactor protein protein protein human Homo sapiens Human MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM DIP protein Q8NDV7 original sequence version: 97 963C054628E18592 wtip_human Wilms tumor protein 1-interacting protein WTIP protein protein human Homo sapiens Human MQRSRAGADEAALLLAGLALRELEPGCGSPGRGRRGPRPGPGDEAAPALGRRGKGSGGPEAGADGLSRGERGPRRAAVPELSAQPAGSPRASLAGSDGGGGGGSARSSGISLGYDQRHGSPRSGRSDPRPGPGPPSVGSARSSVSSLGSRGSAGAYADFLPPGACPAPARSPEPAGPAPFPLPALPLPPGREGGPSAAERRLEALTRELERALEARTARDYFGICIKCGLGIYGAQQACQAMGSLYHTDCFTCDSCGRRLRGKAFYNVGEKVYCQEDFLYSGFQQTADKCSVCGHLIMEMILQALGKSYHPGCFRCSVCNECLDGVPFTVDVENNIYCVRDYHTVFAPKCASCARPILPAQGCETTIRVVSMDRDYHVACYHCEDCGLQLSGEEGRRCYPLAGHLLCRRCHLRRLQPGPLPSPTVHVTEL ago1_human Protein argonaute-1 AGO1 Argonaute RISC catalytic component 1 EIF2C1 Eukaryotic translation initiation factor 2C 1 Putative RNA-binding protein Q99 protein protein human Homo sapiens Human MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA DIP protein Q9UL18 original sequence version: 46 DIP protein Q9UL18 original sequence version: 57 DIP protein Q9UL18 original sequence version: 80 DIP protein Q9UL18 original sequence version: 99 1DBB524AE7CBAF66 ago4_human Protein argonaute-4 AGO4 Argonaute RISC catalytic component 4 EIF2C4 Eukaryotic translation initiation factor 2C 4 KIAA1567 protein protein human Homo sapiens Human MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA F236FF05047534C1 ago3_human Protein argonaute-3 AGO3 Argonaute RISC catalytic component 3 EIF2C3 Eukaryotic translation initiation factor 2C 3 protein protein human Homo sapiens Human MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA DIP protein Q9H9G7 original sequence version: 81 6FF1277995E5322E ddx6_human Probable ATP-dependent RNA helicase DDX6 DDX6 HLR2 RCK DEAD box protein 6 ATP-dependent RNA helicase p54 Oncogene RCK protein protein human Homo sapiens Human MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP DIP protein P26196 original sequence version: 65 DIP protein P26196 original sequence version: 114 DIP protein P26196 original sequence version: 138 F802C642861793FB ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA DIP protein Q9UKV8 original sequence version: 1(def) DIP protein Q9UKV8 original sequence version: 44 DIP protein Q9UKV8 original sequence version: 60 DIP protein Q9UKV8 original sequence version: 62 DIP protein Q9UKV8 original sequence version: 89 DIP protein Q9UKV8 original sequence version: 85 DIP protein Q9UKV8 original sequence version: 87 DIP protein Q9UKV8 original sequence version: 99 DIP protein Q9UKV8 original sequence version: 117 DIP protein Q9UKV8-1 original sequence version: 117 DIP protein Q9UKV8 original sequence version: 145 DIP protein Q9UKV8 original sequence version: 108 5C8552C43FC81345 limd1_human LIM domain-containing protein 1 LIMD1 protein protein human Homo sapiens Human MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQLLQEETLPRGSRGPVNGGGRLGPQARWEVVGSKLTVDGAAKPPLAASTGAPGAVTTLAAGQPPYPPQEQRSRPYLHGTRHGSQDCGSRESLATSEMSAFHQPGPCEDPSCLTHGDYYDNLSLASPKWGDKPGVSPSIGLSVGSGWPSSPGSDPPLPKPCGDHPLNHRQLSLSSSRSSEGSLGGQNSGIGGRSSEKPTGLWSTASSQRVSPGLPSPNLENGAPAVGPVQPRTPSVSAPLALSCPRQGGLPRSNSGLGGEVSGVMSKPNVDPQPWFQDGPKSYLSSSAPSSSPAGLDGSQQGAVPGLGPKPGCTDLGTGPKLSPTSLVHPVMSTLPELSCKEGPLGWSSDGSLGSVLLDSPSSPRVRLPCQPLVPGPELRPSAAELKLEALTQRLEREMDAHPKADYFGACVKCSKGVFGAGQACQAMGNLYHDTCFTCAACSRKLRGKAFYFVNGKVFCEEDFLYSGFQQSADRCFLCGHLIMDMILQALGKSYHPGCFRCVICNECLDGVPFTVDSENKIYCVRDYHKVLAPKCAACGLPILPPEGSDETIRVVSMDRDYHVECYHCEDCGLELNDEDGHRCYPLEDHLFCHSCHVKRLEKRPSSTALHQHHF 085DF06F047B49E6 cnot9_human CCR4-NOT transcription complex subunit 9 Cell differentiation protein RQCD1 homolog RCD1 RQCD1 CNOT9 protein protein human Homo sapiens Human MHSLATAAPVPTTLAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLVKNLQEGQVTDPRGIPLPPQ DIP protein Q92600 original sequence version: 86 CFA0E108F5E9D8F2 ago2-tnrc6a-4 4 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level ser-387 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 2B, 5D, Supp Fig. 4B Interaction was significantly reduced in the absence of LIMD1 limd1-tnrc6a-1 4 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 2B ago2-limd1-1 4 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 2B cnot9-tnrc6a-1 4 18 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 2B ago2-ddx6-4 4 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level ser-387 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 2E, 5F, Supp Fig. 4D Interaction was significantly reduced in the absence of LIMD1 limd1-ago2-2 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 2G ago2-limd1-2 2 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 2H ago2-ddx6-5 2 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 2H ago2-limd1-3 8 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3A, Supp. Fig. 7A limd1-ago2-1 7 16 unspecified role unspecified role prey prey purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 17 unspecified role unspecified role bait bait purified purified region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3C ago2-limd1-4 9 16 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range linker region sufficient to bind sufficient binding region sufficient to bind range range range range Q9UKV8:p.Ser387Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S E Wed Nov 13 12:58:03 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.ser387glu' Tue Jun 30 20:24:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Glu' ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey purified purified region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3E, 4B, Supp Figs. 5b, 5C, 5H Samples were shown to be contaminated with E.coli DnaK but this did not affect AGO2-LIMD1 binding ago2-limd1-5 5 16 unspecified role unspecified role neutral component neutral component purified purified n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9UKV8:p.Ser387Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Fri Feb 01 16:12:02 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p. Ser387Ala' Tue Jun 30 19:55:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Ala' Q9UKV8:p.Ser387Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 19:48:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Glu' 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figures 4C, 4D, 4G, 4H ago2-limd1-6 3 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Fig 2A ago2-limd1-7 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level ser-387 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 17 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 4E The presence of constitutively active myristoylated Akt3 (HA-myr-Akt3) enhanced the interaction of AGO2 with LIMD1. ddx6-ago2-2 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed association association Fig. 4F, Supp. Fig 4G ddx6-ago2-3 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed association association Fig. 4F Cells treated with the Akt inhibitor MK-2206 (CHEBI:67271) ddx6-ago2-4 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 4F, Supp Fig. 4G Interaction observed in LIMD1-/- cells ago2-wtip-1 8 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6F, Supp. Fig. 7B Akt inhibitor MK-2206 (CHEBI:67271) ago2-wtip-2 5 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig. 6H Interaction observed in AKT3-/- cells ago3-wtip-2 4 11 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction Fig. 7B Interaction increased in LIMD1-/- cells ago2-tnrc6a-5 2 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 7D Interaction decreased in LIMD-/- cells, ago3-tnrc6a-1 2 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 7D Interaction increased in LIMD-/- cells, ago2-limd1-8 1 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9UKV8:p.Ser387Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:13:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Ala' Q9UKV8:p.Ser387Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S E Wed Nov 13 12:37:00 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Glu' Tue Jun 30 18:02:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Glu' 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig. 6A, 6B TNRC6A and DDX6 displayed significantly reduced binding to AGO2 Ser387Ala, and rescue of binding with AGO2 Ser387Glu in LIMD1-/- cells ago1-limd1-1 8 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 7A ago3-limd1-1 8 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 7A ago4-limd1-1 8 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 7A ago1-wtip-1 8 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 7B ago3-wtip-3 8 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6F, Supp. Fig. 7B Akt inhibitor MK-2206 (CHEBI:67271) ago4-wtip-1 8 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. Fig. 7B ago1-limd1-2 9 12 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined linker region required to bind necessary binding region required to bind range range range range ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig 7C ago3-limd1-2 5 17 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component purified purified n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9H9G7:p.Glu390Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 15:51:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu390Ala' colocalization colocalization Figures 6B, 6D Interaction observed in AKT3-/- cells ago2-tnrc6a-6 3 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Q9UKV8:p.Ser387Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:28:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Ala' 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Supp. Fig. 2B, 3A, 4A Interaction observed in LIMD-/- cells, but not in LSM1 -/- cells. ago2-ddx6-6 3 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Supp. Fig. 2C, 3B Interaction observed in LIMD-/- cells, but not in LSM1 -/- cells. ago1-limd1-3 5 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9UL18:p.Ser385Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 19:06:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser385Glu' Q9UL18:p.Ser385Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:42:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser385Ala' 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp. Fig. 7D/E Interaction not observed in AKT3-/- cells. The Ser385Glu mutation rescues the interaction in these cells. ago4-limd1-2 5 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9HCK5:p.Ser377Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:12:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser377Ala' Q9HCK5:p.Ser377Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 18:45:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser377Glu' colocalization colocalization Supp. Fig. 7J ago2-24 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 2I ddx6-ago2-5 6 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 2I Interaction observed in lmd-/- cells ago2-25 1 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9UKV8:p.Ser387Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 16:47:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Ala' Q9UKV8:p.Ser387Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 18:42:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser387Glu' 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region xpress tag xpress tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A ago2-tnrc6a-7 3 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Supp. Fig. 3A Interaction observed in LIMD-/- cells, but not in LSM1 -/- cells.