DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the secretin translocation channel in the type II secretion system. Structural insights into the secretin translocation channel in the type II secretion system. Nature structural & molecular biology 2017 imex curation Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn Accepted 2019-MAY-16 AT 08:25 BST AT 08:25 BST by ORCHARD Nature structural & molecular biology 2017 imex curation In Supplementary Figure 7b and c all mutations result in multimer bands disappearing. Hence why I put mutation disrupting (except for G453A that is mutation decreasing). Am I missing a mutation with no effect? Thank you. Structural insights into the secretin translocation channel in the type II secretion system. Structural insights into the secretin translocation channel in the type II secretion system. Nature structural & molecular biology 2017 imex curation Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn Accepted 2019-MAY-16 AT 08:25 BST AT 08:25 BST by ORCHARD Nature structural & molecular biology 2017 imex curation In Supplementary Figure 7b and c all mutations result in multimer bands disappearing. Hence why I put mutation disrupting (except for G453A that is mutation decreasing). Am I missing a mutation with no effect? Thank you. Structural insights into the secretin translocation channel in the type II secretion system. Structural insights into the secretin translocation channel in the type II secretion system. Nature structural & molecular biology 2017 imex curation Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Yan Z., Yin M., Xu D., Zhu Y., Li X. zhuyongqun@zju.edu.cn, lixueming@tsinghua.edu.cn Accepted 2019-MAY-16 AT 08:25 BST AT 08:25 BST by ORCHARD Nature structural & molecular biology 2017 imex curation In Supplementary Figure 7b and c all mutations result in multimer bands disappearing. Hence why I put mutation disrupting (except for G453A that is mutation decreasing). Am I missing a mutation with no effect? Thank you. gspd_ecoli Putative secretin GspD Putative general secretion pathway protein D Putative type II secretion system protein D gspD yheF b3325 JW5707 protein protein ecoli Escherichia coli (strain K12) MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIVGQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVRSANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYEPSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHGKSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYAKATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKLATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQGVADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVTLDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIEQEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDIPLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSKALVGSEDLPVLDENTFNSHAPAPSSR 973259A12A7237B2 gspd_vibch Secretin GspD epsD Cholera toxin secretion protein EpsD General secretion pathway protein D Type II secretion system protein D VC_2733 protein protein vibch Vibrio cholerae MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKVDVRSFDTLNEEQYYSFFLSVLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERANGDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIRRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILISGDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPTTSKRNEVMIAAHADTNSLVLTAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGINLGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTKAVYDTNNNFLRNETTTTKGDYTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEVPVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVMVQDGQMLVLGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKNLMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLRLLDDASVPVLPKFGDDRRHSPEIQAFIEQMEAKQ 3D77B891A59E6223 3D77B891A59E6223 gspd-gspd-2 1 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1c epsd-epsd-2 1 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1e epsd-epsd-1 1 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1f gspd-gspd-1 1 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 1d epsd-epsd-3 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Leu508Asp L484D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L D Tue Jun 30 19:25:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu508Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Leu508Asp L484D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L D Tue Jun 30 17:32:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu508Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7b (L484D lane) epsd-epsd-4 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Val510Asp V486D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 18:23:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val510Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Val510Asp V486D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V D Tue Jun 30 17:12:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val510Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7b (V486D lane) epsd-epsd-5 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Leu522Asp L498D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L D Tue Jun 30 18:43:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu522Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Leu522Asp L498D mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L D Tue Jun 30 18:39:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu522Asp' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7b (L498D lane) epsd-epsd-6 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Ile524Glu I500E mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I E Tue Jun 30 17:49:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile524Glu' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Ile524Glu I500E mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I E Tue Jun 30 18:15:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile524Glu' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7b (I500E lane) epsd-epsd-7 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Ile605Glu I581E mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I E Tue Jun 30 20:01:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile605Glu' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Ile605Glu I581E mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain I E Tue Jun 30 19:15:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile605Glu' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7b (I581E lane) epsd-epsd-8 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Gly477Ala G477A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 16:24:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly477Ala' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Gly477Ala G453A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 17:40:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly477Ala' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7c (G453A lane) epsd-epsd-9 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Gly505Ala G481A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:26:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly505Ala' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Gly505Ala G481A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 16:27:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly505Ala' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7c (G481A lane) epsd-epsd-10 3 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified P45779:p.[Gly477Ala;Gly505Ala] G453A,G481A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 20:14:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly477Ala;Gly505Ala]' strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.[Gly477Ala;Gly505Ala] G453A,G481A mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 16:33:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly477Ala;Gly505Ala]' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supplementary Figure 7c (G453A&G481A lane) gspd-6 2 4 structure determination identification by structure determination identification by structure determination structure determination ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure1, Table1, Supplementary Figure 2 epsd-11 2 5 structure determination identification by structure determination identification by structure determination structure determination ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified p.Gly371_Ala393del 371-393 mutation mutation certain certain sequence position certain certain certain sequence position certain GSVIQYGNTGASIGNVMIGLEEA . strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure1, Table1, Supplementary Figure 2 epsd-1 2 5 structure determination identification by structure determination identification by structure determination structure determination ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P45779:p.Gly477Ala G453A mutation mutation certain certain sequence position certain certain certain sequence position certain G A The mutation occurs on the 477th position on the original sequence (the sequence used in this paper starts from the 24th AA). Tue Jun 30 15:38:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly477Ala' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure1, Table1, Supplementary Figure 2 epsd-12 2 5 structure determination identification by structure determination identification by structure determination structure determination ms participant Identification by mass spectrometry ms participant experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure1, Table1, Supplementary Figure 2