IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A homozygous point mutation in the GH1 promoter (c.-223C>T) leads to reduced GH1 expression in siblings with isolated GH deficiency (IGHD). A homozygous point mutation in the GH1 promoter (c.-223C>T) leads to reduced GH1 expression in siblings with isolated GH deficiency (IGHD). European journal of endocrinology 2016 imex curation Madeira JL., Jorge AA., Martin RM., Montenegro LR., Franca MM., Costalonga EF., Correa FA., Otto AP., Arnhold IJ., Freitas HS., Machado UF., Mendonca BB., Carvalho LR. lucianic@gmail.com 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Madeira JL., Jorge AA., Martin RM., Montenegro LR., Franca MM., Costalonga EF., Correa FA., Otto AP., Arnhold IJ., Freitas HS., Machado UF., Mendonca BB., Carvalho LR. lucianic@gmail.com Accepted 2019-JUN-17 AT 08:36 BST AT 08:36 BST by LIVIAP European journal of endocrinology 2016 Only protein-protein interactions imex curation imex curation Participant detection method corrected pit1_rat Pituitary-specific positive transcription factor 1 Pou1f1 Ghf-1 Pit-1 Pit1 Growth hormone factor 1 protein protein rat Rattus norvegicus (Rat) Rat MSCQPFTSADTFIPLNSDASAALPLRMHHSAAEGLPASNHATNVMSTATGLHYSVPSCHYGNQPSTYGVMAGTLTPCLYKFPDHTLSHGFPPLHQPLLAEDPTASEFKQELRRKSKLVEEPIDMDSPEIRELEQFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEHSKPSSQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR 2651876B2F7668A1 gh1_gene human_gh1_gene nucleic acid nucleic acid human Homo sapiens Human TGCCTGTAATCCCAGCATTTTGGTAGGCCAAGGCGGGTGGATCACCTGAGGTCAGGAGTTCGAGGCCAGCCTGATGACCATGGTGAAACCCCATCTCTACTAAAAATACAAAATTAATCGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTAAACCCAGGAAGTGGAGGTTTCAGTGAGCTGAGATTGTGCCATTGCACTCCAGCCTGGGTAACAAGAGCAAAACTCCATCAAAAAAAATAATATATGTATATATATATTACAATTTTATATATATATACACATTATGTAATTTTTTTTACCATTTTATATATATACATTACGTAATGGTAAAATGTTTTCTCTGCCCCCCCAGTAGATTGTTAGCTCCAGAAGAGAAAGGATCATGTCTTTGGTTTATCTAGATATGTCCATCGGCCTGGTACAGTCTCTGGCCCATGTTATAGGCAACAACTACTTGTAGAATCGGTGAATGCATGAATAGAAGAATGAGTGAATGAATGAATAGACGAAAGGCAGAAATCCAGCCTCAGGCCGGGCGTGGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACAAGATCAAGAGATGGAGACCAACCTGGCTAACACAGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGCCGGGCATGGCGGTAGGCTCCTGTAGTCCGAGCTACTAGGGAGCCTGAGGCAGGAGAATGGCATGAACCCAGGGGGGCGGAGCTTGCAGTGAGCCAAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTCGGTCTCAAAAAAAAAAAAAAAAGAAATCCAGCCTCAAAGAGCTTACAGTCTGGTAAGAGGAATAAAATGTCTGCAAATAGCCACAGGACAGGTCAAAGGAAGGAGAGGCTATTTCCAGCTGAGGGCACCCCATCAGGAAAGCACCCCAGACTTCCTACAACTACTAGACACATCTCGATGCTTTTCACTTCTCTATCAATGGATCGTCTCCCTGGAGAATAATCCCCAATGTGAAATTACTTAGCACGTCAAGTTAGGTAGATCCTTGTGTACTTCTTGGTTGTTCAGAGATCATCAACCAGTGCAAACAATCCCCCCATCAATACACAGCAGTGTCTGCCCCTCTCCTCCCCAAGCCTTCCGAGGCCCTTCCTCCGTGCCTGAACCCCCTGGACATATCATGTGGCAAACTGAAGGGTCCAACGAGATACAGGAAGTGAAACACGATGTACACTGAAACGTGCAATACAAATATGCAGCATGAAGTGCCTCGGTTCACTAACCCGAGCTATGCTGGGTGCTTCTTTTCTACCACTTTCCTTAATGCCTATGGACACCTCATTCTGTGGCTGAAGTTCCTTGTGTTCAATTCCCCCCATCTTCATTGAACATCCTCTGTGCAGGGACTTGACCCCTGTCCTGCTAGCTTTGCACTGAGGCAAGTTTTGTCCATGCCTAGTAGTGCCACCATCTTTACTAGATGAGGTTTCTAAAGAGCTTGGCATGGAAGGAAAGCCTGGGGGGCCTTAGAAGCCATCACTTAGGAACTGGGAGAGCTCCAGGCAAGCCACCTTATCTCTTTGGGCCTCAGTATCCTCACTCACATCCCCATGGTCAGTAGAGTGCCAAGCACATAGTGGACACGCAGAAGTATTGATCATCTTCCTCACCTCCCCTAGGAGAGCCCTTGGGAATCCCCATATAATATTCTCTGAAGACCTCAATCCAATGAGCCAAGTAATTGACTCGGTAGCAAACTCATGGAAAGGACATTAGAAGCCAAAAGGAGGTAGAGTATGTCCCAGTACAATAACCAGCCTCTGATCTCAAGGAAGAAAGAACAGAGCTGCAGGTGAGAAGTGTGCGCCTCAAATCACCAAAGTGAGAGTGGAAGGAGCACCCACCAGTCCCTTGGAGGCGATCCCTAAGACCGGTGAGAATGGCTTCGAAAAATGTGATCGTCCTCAACCCCACAGTCTTGAACCTAACAGGAGATCTTGAAGCCTGAAAGACACCACTTTAGGATCAACAGCAGATCTGTGACTTTCCACAGCAGGCACACAGAACCCTAGAGTTAGTCGAGGTTGGACATAGGAAGGGCTTCCAAACACACAGAGAAATTCATGGATCCTAAATTATAAAGGGAGGTTCTTTAAAAGAACCAAGATGATTCTGAGATTTATCCTGAGCTTTTTTTTTTTTTTTTTTTTGGATGCAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTAACTGCAACCTCCACCTCGCGGGTTCAAGCAATTCCCTGCCTCGGCCTCCTGAATAGCTGGGATTACAGGCGCCTGCCACCACACCTGGCTAATTTTTGTATTTTTAGCAGAGACGGGATTTCACCATCTTGGCCAGGCTGGTCTTGAACTCCTGATCTCATGATCCACCCACCTTGGCCTCCCTAAGTGGTGGGATTACAGGCGTGTGCCCAACTTTTCCTGAGCCTTTTGAGGCTGACACCAGAGGTGAGAAGCCCAGCCTCTCCCCACTGGCCATGTGGGGAGAGGCTCCAGCCTGCAGCAACCAGGGATCTGGCCTCAAGTGATGCCCCAACAGTGGGCGACTTCCCAGTACTGTTAGGAGAATCCCAAGTCTAATTCAAAGTTGATTTTTTACTAGCAATGAATGATAGACATGGTCTCCATTGCTCAAGGCTCTGGGAAGATCTAGACTAGAGAAAACGATCACCGACTTCTACCCACACCTGTGGGCCTCAGTTCTTCCCTCTGGTCCATGGTTACCACAGTAACCCCTTGTAAAGGTGTTTCCCCAGGGGTCCCTAGAGTCCTCTGAGTCACCATAATTCTGGGTCAACAGAAATGGAGAGGTAAGAGGAGACACTCCCCTGCCCTGGCTGGTCCCACGTGTTCATGGCAGTAACCTGTCTGGGGAGCCTCGCCACCCCTGTGTCCACCTGCAGAGTTATGCAGTGGTCCCCAACTAGGGCCCCTGCCACCCTCATTCCTAAGGGAGGCTGGAGACTTCTTCCATGGCCGAAAATCCACATCTAAGTCCCCGGCACTAGCAAAAAGGCCCTGTCATGGGGGCTCCCTGCCCCAGTTGAGATTGGAGGAACATGGAAGAAGCTAAAATAACTAAATAACTCAGTAGCATCACACTACCTGCTCTGGAGAAAAAAAAATTTAATATTATTATTTTTTGTTTTTTGAGATGGAGTTCTGCTCTGCTGCACAGGCTGGAGTGCAGTGGCGCAATCTTGGCTCACGGCAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCCGCCTCAGCCTCCCGAGTAGTTGGGATTACAGGCTCATGCCACCACGCCCAGCTAATTTTTGTACTTTTAGTAGAGATGGAGTTTTGCCATGTTGGCTAGTCTGGCCTTGAACTCCTGACCTCAAGTGATCCACCCACCTCAAAGCCACCCAAAGTTTGGGGATTACAAGCGTGAGCCACTGTGTCCGGCCTGGAGAAAGGACTTTAAATGACGCAATGTAGGAAGAGCAAGGTTGTGGAGATCTGCTGCCCTGGCTGAGGTAGCTCATGCAATCAGTCTCTCTGAGCCACAGTCTCTTGATCTGTGAAATCGGAAGAAAATAATACCTCCTTCACAAGACAAGTGGCAGGTCAGATGTGAGAATGCACAGGCAGGCCCTCGGCAACTGGAAAAGCTCTATACAGATCTGAAAAGGAGGAGGAGAAAAAAGAGGAGGGGCTTCCATGGCTGGACAGGGCATCTTTCTTTTTCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTGAGGTGGAGTCTTGCTCTGTTGCCAAGGTTGGAGTGCAGCAGCACGATCTCCGCTCACTGCAAGCTCTGCCTCCCGGATTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGAATACAGGCGCCCGCCACTACGCCCAGCTAACTTTTTTGCATTTTTAGTACAGAGTGGATTTCACCTGGTTAGCCAAGATGGTCTTGATCTACTGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCAGCCTGATAGAGCATCTTTCGGCGTGATGTGTTCTGAGTTCCAAAGCTGAGGAAGAGACTCAAATCTTCAAGAGCTCTTCTAACTTTGAGATTCTCTGATGGTTTCAGGGCTATGGGAGGAAGAGCTTGTGGTCCGTGTCTGCTCCCGGGATTTCTGTTTCTTGGTTTGTGTCTCTGCTGCAAGTCCAAGGAGCTGGGGCAATACCTTGAGTCTGGGTTCTTCGTCCCCAGGGACCTGGGGGAGCCCCAGCAATGCTCAGGGAAAGGGGAGAGCAAAGTGTGGGGTTGGTTCTCTCTAGTGGTCAGTGTTGGAACTGCATCCAGCTGACTCAGGCTGACCCAGGAGTCCTCAGCAGAAGTGGAATTCAGGACTGAATCGTGCTCACAACCCCCACAATCTATTGGCTGTGCTTGGCCCCTTTTCCCAACACACACATTCTGTCTGGTGGGTGGAGGTTAAACATGCGGGGAGGAGGAAAGGGATAGGATAGAGAATGGGATGTGGTCGGTAGGGGGTCTCAAGGACTGGCTATCCTGACATCCTTCGCCGCGTGCAGGTTGGCCACCATGGCCTGCGGCCAGAGGGCACCCACGTGACCCTTAAAGAGAGGACAAGTTGGGTGGTATCTCTGGCTGACACTCTGTGCACAACCCTCACAACACTGGTGACGGTGGGAAGGGAAAGATGACAAGCCAGGGGGCATGATCCCAGCATGTGTGGGAGGAGCTTCTAAATTATCCATTAGCACAAGCCCGTCAGTGGCCCCATGCATAAATGTACACAGAAACAGGTGGGGTCAACAGTGGGAGAGAAGGGGCCAGGGTATAAAAAGGGCCCACAAGAGACCAGCTCAAGGATCCCAAGGCCCAACTCCCCGAACCACTCAGGGTCCTGTGGACAGCTCACCTAGCTGCAATGGCTACAGGTAAGCGCCCCTAAAATCCCTTTGGGCACAATGTGTCCTGAGGGGAGAGGCAGCGACCTGTAGATGGGACGGGGGCACTAACCCTCAGGTTTGGGGCTTCTGAATGTGAGTATCGCCATGTAAGCCCAGTATTTGGCCAATCTCAGAAAGCTCCTGGTCCCTGGAGGGATGGAGAGAGAAAAACAAACAGCTCCTGGAGCAGGGAGAGTGCTGGCCTCTTGCTCTCCGGCTCCCTCTGTTGCCCTCTGGTTTCTCCCCAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGCCCTGGCTTCAAGAGGGCAGTGCCTTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATGCTCCGCGCCCATCGTCTGCACCAGCTGGCCTTTGACACCTACCAGGAGTTTGTAAGCTCTTGGGGAATGGGTGCGCATCAGGGGTGGCAGGAAGGGGTGACTTTCCCCCGCTGGGAAATAAGAGGAGGAGACTAAGGAGCTCAGGGTTTTTCCCGAAGCGAAAATGCAGGCAGATGAGCACACGCTGAGTGAGGTTCCCAGAAAAGTAACAATGGGAGCTGGTCTCCAGCGTAGACCTTGGTGGGCGGTCCTTCTCCTAGGAAGAAGCCTATATCCCAAAGGAACAGAAGTATTCATTCCTGCAGAACCCCCAGACCTCCCTCTGTTTCTCAGAGTCTATTCCGACACCCTCCAACAGGGAGGAAACACAACAGAAATCCGTGAGTGGATGCCTTCTCCCCAGGCGGGGATGGGGGAGACCTGTAGTCAGAGCCCCCGGGCAGCACAGCCAATGCCCGTCCTTCCCCTGCAGAACCTAGAGCTGCTCCGCATCTCCCTGCTGCTCATCCAGTCGTGGCTGGAGCCCGTGCAGTTCCTCAGGAGTGTCTTCGCCAACAGCCTGGTGTACGGCGCCTCTGACAGCAACGTCTATGACCTCCTAAAGGACCTAGAGGAAGGCATCCAAACGCTGATGGGGGTGAGGGTGGCGCCAGGGGTCCCCAATCCTGGAGCCCCACTGACTTTGAGAGCTGTGTTAGAGAAACACTGCTGCCCTCTTTTTAGCAGTCAGGCCCTGACCCAAGAGAACTCACCTTATTCTTCATTTCCCCTCGTGAATCCTCCAGGCCTTTCTCTACACCCTGAAGGGGAGGGAGGAAAATGAATGAATGAGAAAGGGAGGGAACAGTACCCAAGCGCTTGGCCTCTCCTTCTCTTCCTTCACTTTGCAGAGGCTGGAAGATGGCAGCCCCCGGACTGGGCAGATCTTCAAGCAGACCTACAGCAAGTTCGACACAAACTCACACAACGATGACGCACTACTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATCGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTAAAATAACTATACCAGCAGGAGGACGTCCAGACACAGCATAGGCTACCTGGCCATGCCCAACCGGTGGGACATTTGAGTTGCTTGCTTGGCACTGTCCTCTCATGCGTTGGGTCCACTCAGTAGATGCCTGTTGAATTCCTGGGCCTAGGGCTGTGCCAGCTGCCTCGTCCCGTCACCTTCTGGCTTCTTCTCTCCCTCCATATCTTAGCTGTTTTCCTCATGAGAATGTTCCAAATTCGAAATTTCTATTTAACCATTATATATTTACTTGTTTGCTATTATCTCTGCCCCCAGTAGATTGTTAGCTCCAGAAGAGAAAGGATCATGTCTTTTGCTTATCTAGATATGCCCATCTGCCTGGTACAATCTCTGGCACATGTTACAGGCAACAACTACTTGTGGAATTGGTGAATGCATGAATAGAAGAATGAGTGAATGAATGAATAGACAAAAGGCAGAAATCCAGCCTCAAAGAGCTTACAGTCTGGTAAGAGGAATAAAATGTCTGCAAATAGCCACAGGACAGGTCAAAGGAAGGAGGGGCTATTTCCAGCTGAGGGCACCCCATCAGGAAAGCACCCCAGACTTCCTACAACTACTAGACACATCTCGATGCTTTTCACTTCTCTATCAATGGATCGTCTCCCTGGAGAATAATCCCCAAAGTGAAATTACTTAGCACGTCCAGTTAGGTAGATCCTTGTGTACTTCTTGGTTGTTCAGAGATCATCAACCAGTGCAAACAATCCCCCCATCAATACACAGCAGTGCCTGCCCCTCTCCCCCCGAGGTCTTCCGAGGCCCTTCCTCCGTGCCTGAACCCCCTGGACATATCATATGGCAAACTGAAGTGTCCAACGAGATATAGGAAGTGAAACACGATGTACACTGAAACGTGCAATACAAATATGCAGCATGAAGTGCCTCGGTTCACTAACCCGAGCTACGCTGGGTGCTTCTTTTCTACCACTTTCCTTAATGCCTATGGACACCTCATTCTGTGGCTGAAGTTCCTTGTGTTCAATTCCCCCCATCTTCATTGAACATCCTGTGTAGGGACCTCACCCCTGTCCTGCTAGCTTTGCACTGAGGCAAGTTCTGTCCATGCCTAGTAGTGCCACCACCTTTACTAGATGAGACTTCTAAAGAGCTTGGCATGGAAGGAAAGCCCGGGGGCCTTGGAAGCCATCACTTAGAAACTGGGAGAGCTCCAGGCAAGCCACCTCATCTCTTTGAGCCTCAGTATCCTCACTCACATCCCTATGGTCAGTAGAGTGCCAAGCACATGGTGGACACGCAGAAGTATTGATCATCTTCCTCACCTCCCCTAGGAGAGCCCTTGGGAATCCCCATATAATATTCTCTGAAGACCTCAATCCAATGAGCCAAGTAATTGACTCGGTAGCAAACTCATGGAAAGGACATTAGAAGCCAAAAGGAGGTAGAGTATGTCCCAGTACAATAACCAGCCTCTGATCTCAAGGAAGAAAGAACAGAGCTGCAGGTGAGAAGTGTGCGCCTCAAATCACCAAAGTGAGAGTGGAAGGAGCACCCACCAGTCCCTTGGAGGCGATCCCTAAGACCGGTGAGAATGGCTTCGAAAAATGTGATCGT gh1_gene-pou1f1-1 1 3 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined promoter region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain TGTGTGGGAGGAGCTTCTAAATTATCCATTAGCAC g.4878T>C mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T C genomic co-ordinates of the variant: 17:63918961 chemical synthesis Chemical synthesis (Chemical synthesis) 2 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep cell lysate cell lysate rat-gh3 Rat pituitary tumor GH3 cells gh3 Rat pituitary tumor GH3 cells physical association physical association Figure 5