IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nature communications 2014 imex curation Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-cardiac myocyt Mus musculus cardiac_myocyte cardiac myocyte confocal microscopy confocal microscopy immunostaining immunostaining Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca Accepted 2019-APR-24 AT 15:46 BST AT 15:46 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation Colocalization taken as two interactions; tmm65-cxa1 and tmm65-desp | GO X'ref added Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nature communications 2014 imex curation Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-cardiac myocyt Mus musculus cardiac_myocyte cardiac myocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sharma P., Abbasi C., Lazic S., Teng AC., Wang D., Dubois N., Ignatchenko V., Wong V., Liu J., Araki T., Tiburcy M., Ackerley C., Zimmermann WH., Hamilton R., Sun Y., Liu PP., Keller G., Stagljar I., Scott IC., Kislinger T., Gramolini AO. thomas.kislinger@utoronto.ca,anthony.gramolini@utoronto.ca Accepted 2019-APR-24 AT 15:46 BST AT 15:46 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation Colocalization taken as two interactions; tmm65-cxa1 and tmm65-desp | GO X'ref added cxa1_mouse Gap junction alpha-1 protein Gja1 Cxn-43 Connexin-43 Gap junction 43 kDa heart protein protein protein mouse Mus musculus Mouse MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDSQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI DIP protein P23242 original sequence version: 72 DIP protein P23242 original sequence version: 132 DIP protein P23242 original sequence version: 153 018DCB461FA69490 desp_mouse Desmoplakin Dsp protein protein mouse Mus musculus Mouse MSCNGGSHPRINTLGRMTRAESGPDLRYEMTYSGGGGGGGGGGGGGTSRTFYSHSRRCTVNDQNSDGYCQTGTMSRHQNQNTIQEMLQNCSDCLMRAELIAQPELKFGEGMQLAWNRELDEYFTQANDQMEIIDGLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGAGGYTCQSGSGWDEFTKRLTGECLGWMRQQREEMDLMAWGVDAGSVEQHINSHRSIHNTIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSIRKKYPCDKNMPLQHLLEQIKELEKEREKIIEYKRQVQNLVNKSKKIVQLKPRNPDYRSNKPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNPLAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDYMKTIEDLELHYQDFIKNSQGSEMFGDDDKRRMQSQFTDAQKHYQTLVIQLPGHPQHQTVTKTEITHLGTCQDVNHNKVIETNRENDKQETWLLMELQKIRRQMEHCEARMTLKNLLLAEQGSTHHITVKINELKSVQNDSQALAEVLNQLKDMLANFRGSEKYCYLQNEIFGLFQKLENINGVSDGYLNSLCSVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLKKIKNDLNLKKSLLATMKTELQKAQQIHSQSSQQYPLYDLDLGKFTEKVTQLTDRWQKIDKQIDFRLWDLEKQIKQLRNYRDNYQSFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQKNLHSEISGKRDKSEEVHKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMVQSPSGVILQEAADIHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDANSENCNKNKFLDQNLQKYQAECSQFKAKLVSLEELKRQAELDGKSAKQNLDKCYGQIKELNEKITRLTYEIEDEKRRRKTVEDRFDQQKNDYDQLQKARQCEKENLSWQKLESEKAIKEKEYEIERLRVLLQEEGARKREYENELAKVRNHYNEEMSNLRNKYETEINITKTTIKEISMQKEDDSKNLRNQMDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQQKACGSETMQKKQRLEIELKQVIQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEYQEEAKRRWEYENELSKVRNSYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQIDNLTRENRSLCEEVKRLKNTLAQTTENLRRVEENAQQQKATGSEMSQRKQQLEIELRQVTQMRTEESMRYKQSLDDAAKTIQDKNKEIERLKQLVDKETNERKCLEDENSKLQRVQYDLQKANNSATEAMSKLKVQEQELTRLRIDYERVSQERTVKDQDITRIQSSLKDLQLQKQKAEEELSRLKRTASDESSKRKMLEEELEAMRRSLKEQAVKITNLTQQLEQASIVKKRSEDDLRQQRDVLDGHVREKQRTQEELRRLSLDVEALRRQLVQEQENVKQAHLRNEHFQKAIEDKSRSLNESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNSTISELRSQLQISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKSQCTQVVQERESLLVKIKVLEQDKARLQRLEDELNRAKATLEAESRVKQRLECEKQQIQNDLNQWKTQYSRKEETIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSSRETQSQLESERCRLQKEIEKLRQRPYGSHRETQTEYEWTVDSSKLVFDGLRKKVTAMQLYECQLIDKTTLDKLLKGKKSVEEVASEIQPFLRGAGAIAGASASPKEKYSLVEAKRKKFITPESTVMLLEAQAATGGIIDPHRNEKLTVDNAVARDLIDFDDRQQIYTAEKAITGFDDPFSGKTVSVSEAIKKNLIDRETGMRLLEAQLASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRDSQKNFVDPITKKKVSYMQLRERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSGILRPSTVNELESGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRPGTALELLEAQAATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPETGNIISLFQAMNKELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDMAYKRGYFNEELSEILSDPSDDTKGFFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLRKRRVVIVDPETNKEMSVQEAYKKGLIDYDTFKELCEQECEWEEITITGSDGSTRVVLVDRKTGSQYDIQDAIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGNSSGLGGSVNDDVFSSSRHDSVSKISTISSVRNLTIRSSSLSDPLEESSPIAAIFDTENLEKISIAEGIERGIVDSITGQRLLEAQACTGGIIHPTTGQKLSLQDAVNQGLIDQDMATRLKPAQKAFIGFEGVKGKKKMSAAEAVKEKWLPYEAGQRFLEFQFLTGGLVDPEVHGRISTEEAIRKGFIDGRAAQRLQDISSYAKILTCPKTKLKISYKDAMNRSMVEDITGLRLLEAASVSSKGLPSPYNMSAPGSRSGSRSGSRSGSRSGSRSGSRRGSFDATGNSSYSYSYSFSSSSIGGY AfCS Y2H protein. Translated from nucleotide sequence. Similar to DESP_HUMAN Desmoplakin (DP) (250/210 kDa paraneoplastic pemphigus antigen) (95%) 1D8B68788D519940 tmm65_mouse Transmembrane protein 65 Tmem65 protein protein mouse Mus musculus Mouse MSRLLPLLGSRTARSLRPGPAAAPRLPSWCCCGRGLLALGVPGGPRLLGTHPKKEPMEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEALPPTPGQLRYVFFHNAIPFVGFGFLDNAIMIVAGTQIELSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRVSTHLGKAVGVTIGCILGMFPLIFFGGSEEDEKLETTN 619D47EDE37C1396 cdh2-dsp-1 1 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Figure 6a gja1-tmem65-1 2 5 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 6b The Tmem65 monomer and dimer co-immunoprecipitated with Cx43, although significant enrichment was observed in the dimer, compared with levels seen in the input cardiac lysate. dsp-tmem65-1 2 5 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 6b gja1-tmem65-2 1 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 3 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Figure 6a