IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. The Journal of biological chemistry 2015 imex curation Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-nlt NLT-immortalized GnRH-expressing cell line nlt NLT anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu Accepted 2019-SEP-26 AT 13:23 BST AT 13:23 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. The Journal of biological chemistry 2015 imex curation Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-gt1_7 Mus musculus hypothalamic cell line, GT1-7 gt1_7 mouse hypothalamic cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu Accepted 2019-SEP-26 AT 13:23 BST AT 13:23 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. The Journal of biological chemistry 2015 imex curation Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-nlt NLT-immortalized GnRH-expressing cell line nlt NLT anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Salian-Mehta S., Xu M., McKinsey TA., Tobet S., Wierman ME. No Species of origin can be found for HA-HDAC9 so no interaction entered - figure 5A;similarly for figures 5B/C exogenous interactions. The authors did not reply to a request for information. N-terminally FLAG tagged HDAC9 was found to be of Human origin from pmid: 12590135, and to be Isoform 7 (Uniprot Q9UKV0-7) based on length and sequence as given in this publication. (1069aas) HDAC6 as used in figure 5A was found to be of Human origin based on sequence length:- pcDNA3.1(+) full-length FLAG-HDAC6 (1–1215), truncated FLAG-HDAC6 (1–503 and 1–840), FLAG-HDAC9-HA, and HA-HDAC6 constructs were provided by Xiaohong Zhang and Edward Seto. In figure 5B HDAC has been entered as being of Human origin based on mutation mapping. margaret.wierman@ucdenver.edu Accepted 2019-SEP-26 AT 13:23 BST AT 13:23 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hdac9_mouse Histone deacetylase 9 Histone deacetylase 7B Histone deacetylase-related protein MEF2-interacting transcription repressor MITR Hdac9 Hdac7b Hdrp Mitr protein protein mouse Mus musculus Mouse MHSMISSVDVKSEVPMGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKELLAIKQQQELLEKEQKLEQQRQEQEVERHRREQQLPPLRGKDRGRERAVASTEVKQKLQEFLLSKSATKDTPTNGKNHSVGRHPKLWYTAAHHTSLDQSSPPLSGTSPSYKYTLPGAQDSKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNLVTSFKKRVFEVAESSVSSSSPGSGPSSPNNGPAGNVTENEASALPPTPHPEQLVPQQRILIHEDSMNLLSLYTSPSLPNITLGLPAVPSPLNASNSLKDKQKCETQMLRQGVPLPSQYGSSIAASSSHVHVAMEGKPNSSHQALLQHLLLKEQMRQQKLLVAGGVPLHPQSPLATKERISPGIRGTHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKLLSKSIEQLKQPGSHLEEAEEELQGDQSMEDRAASKDNSARSDSSACVEDTLGQVGAVKVKEEPVDSDEDAQIQEMECGEQAAFMQQVIGKDLAPGFVIKVII DIP protein Q99N13 original sequence version: 84 4ED7FA9F02BD4621 q9ukv0-7 Histone deacetylase 9 HDAC9 HDAC7 HDAC7B HDRP KIAA0744 MITR Histone deacetylase 7B Histone deacetylase-related protein MEF2-interacting transcription repressor MITR protein protein human Homo sapiens Human MHSMISSVDVKSEVPVGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKLQQELLAIKQQQELLEKEQKLEQQRQEQEVERHRREQQLPPLRGKDRGRERAVASTEVKQKLQEFLLSKSATKDTPTNGKNHSVSRHPKLWYTAAHHTSLDQSSPPLSGTSPSYKYTLPGAQDAKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNVVTSFKKRMFEVTESSVSSSSPGSGPSSPNNGPTGSVTENETSVLPPTPHAEQMVSQQRILIHEDSMNLLSLYTSPSLPNITLGLPAVPSQLNASNSLKEKQKCETQTLRQGVPLPGQYGGSIPASSSHPHVTLEGKPPNSSHQALLQHLLLKEQMRQQKLLVAGGVPLHPQSPLATKERISPGIRGTHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKLLSKSIEQLKQPGSHLEEAEEELQGDQAMQEDRAPSSGNSTRSDSSACVDDTLGQVGAVKVKEEPVDSDEDAQIQEMESGEQAAFMQQPFLEPTHTRALSVRQAPLAAVGMDGLEKHRLVSRTHSSPAASVLPHPAMDRPLQPGSATGIAYDPLMLKHQCVCGNSTTHPEHAGRIQSIWSRLQETGLLNKCERIQGRKASLEEIQLVHSEHHSLLYGTNPLDGQKLDPRILLGDDSQKFFSSLPCGGLGVDSDTIWNELHSSGAARMAVGCVIELASKVASGELKNGFAVVRPPGHHAEESTAMGFCFFNSVAITAKYLRDQLNISKILIVDLDVHHGNGTQQAFYADPSILYISLHRYDEGNFFPGSGAPNEVGTGLGEGYNINIAWTGGLDPPMGDVEYLEAFRTIVKPVAKEFDPDMVLVSAGFDALEGHTPPLGGYKVTAKCFGHLTKQLMTLADGRVVLALEGGHDLTAICDASEACVNALLGNELEPLAEDILHQSPNMNAVISLQKIIEIQSKYWKSVRMVAVPRGCALAGAQLQEETETVSALASLTVDVEQPFAQEDSRTAGEPMEEEPAL Phosphorylated on Tyr-1010 5DFBB8DD4C6547C5 hdac6_human Histone deacetylase 6 HDAC6 KIAA0901 JM21 Tubulin-lysine deacetylase HDAC6 protein protein human Homo sapiens Human MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH 6F17731268A33114 hdac6_mouse Histone deacetylase 6 Histone deacetylase mHDA2 Hdac6 Tubulin-lysine deacetylase HDAC6 protein protein mouse Mus musculus Mouse MTSTGQDSSTRQRKSRHNPQSPLQESSATLKRGGKKCAVPHSSPNLAEVKKKGKMKKLSQPAEEDLVVGLQGLDLNPETRVPVGTGLVFDEQLNDFHCLWDDSFPESPERLHAIREQLILEGLLGRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLAETYDSVYLHPNSYSCACLATGSVLRLVDALMGAEIRNGMAVIRPPGHHAQHNLMDGYCMFNHLAVAARYAQKKHRIQRVLIVDWDVHHGQGTQFIFDQDPSVLYFSIHRYEHGRFWPHLKASNWSTIGFGQGQGYTINVPWNQTGMRDADYIAAFLHILLPVASEFQPQLVLVAAGFDALHGDPKGEMAATPAGFAHLTHLLMGLAGGKLILSLEGGYNLRALAKGVSASLHTLLGDPCPMLESCVVPCASAQTSIYCTLEALEPFWEVLERSVETQEEDEVEEAVLEEEEEEGGWEATALPMDTWPLLQNRTGLVYDEKMMSHCNLWDNHHPETPQRILRIMCHLEEVGLAARCLILPARPALDSELLTCHSAEYVEHLRTTEKMKTRDLHREGANFDSIYICPSTFACAKLATGAACRLVEAVLSGEVLNGIAVVRPPGHHAEPNAACGFCFFNSVAVAARHAQIIAGRALRILIVDWDVHHGNGTQHIFEDDPSVLYVSLHRYDRGTFFPMGDEGASSQVGRDAGIGFTVNVPWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLISAGFDAAQGDPLGGCQVTPEGYAHLTHLLMGLAGGRIILILEGGYNLASISESMAACTHSLLGDPPPQLTLLRPPQSGALVSISEVIQVHRKYWRSLRLMKMEDKEECSSSRLVIKKLPPTASPVSAKEMTTPKGKVPEESVRKTIAALPGKESTLGQAKSKMAKAVLAQGQSSEQAAKGTTLDLATSKETVGGATTDLWASAAAPENFPNQTTSVEALGETEPTPPASHTNKQTTGASPLQGVTAQQSLQLGVLSTLELSREAEEAHDSEEGLLGEAAGGQDMNSLMLTQGFGDFNTQDVFYAVTPLSWCPHLMAVCPIPAAGLDVSQPCKTCGTVQENWVCLTCYQVYCSRYVNAHMVCHHEASEHPLVLSCVDLSTWCYVCQAYVHHEDLQDVKNAAHQNKFGEDMPHSH DIP protein Q9Z2V5 original sequence version: 131 D5F73BA3F79AF520 hdac6-hdac9-1 2 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 4A,5D Tubastatin A - an HDAC6 inhibitor hdac6-hdac9-2 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 4B hdac9-hdac6-1 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 4I hdac6-hdac9-3 3 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9UBN7:p.His216Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 20:21:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His216Ala' Q9UBN7:p.His611Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 16:38:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His611Ala' Q9UBN7:p.[His216Ala;His611Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 17:36:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His216Ala;His611Ala]' 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 5B