IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. The Journal of biological chemistry 2015 imex curation Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr Accepted 2019-MAY-23 AT 08:33 BST AT 08:33 BST by LIVIAP The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation done Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. The Journal of biological chemistry 2015 imex curation Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr Accepted 2019-MAY-23 AT 08:33 BST AT 08:33 BST by LIVIAP The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation done Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. Convergence of melatonin and serotonin (5-HT) signaling at MT2/5-HT2C receptor heteromers. The Journal of biological chemistry 2015 imex curation Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Kamal M., Gbahou F., Guillaume JL., Daulat AM., Benleulmi-Chaachoua A., Luka M., Chen P., Kalbasi Anaraki D., Baroncini M., Mannoury la Cour C., Millan MJ., Prevot V., Delagrange P., Jockers R. Fig 5E?F:-Refs 11/23 gives several possible constructs for the Barr sensor; the reaction is intramolecular - self reacting Luc–beta-arr–YFP fusion protein, most likely. No direct detection of the Melatonin receptor or HTR2C is involved , so not appropriate to annotate. Details regarding YFP tags from pmid: 11940583 via Ref 12. ralf.jockers@inserm.fr Accepted 2019-MAY-23 AT 08:33 BST AT 08:33 BST by LIVIAP The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation done mtr1a_human Melatonin receptor type 1A MTNR1A protein protein human Homo sapiens Human MQGNGSALPNASQPVLRGDGARPSWLASALACVLIFTIVVDILGNLLVILSVYRNKKLRNAGNIFVVSLAVADLVVAIYPYPLVLMSIFNNGWNLGYLHCQVSGFLMGLSVIGSIFNITGIAINRYCYICHSLKYDKLYSSKNSLCYVLLIWLLTLAAVLPNLRAGTLQYDPRIYSCTFAQSVSSAYTIAVVVFHFLVPMIIVIFCYLRIWILVLQVRQRVKPDRKPKLKPQDFRNFVTMFVVFVLFAICWAPLNFIGLAVASDPASMVPRIPEWLFVASYYMAYFNSCLNAIIYGLLNQNFRKEYRRIIVSLCTARVFFVDSSNDVADRVKWKPSPLMTNNNVVKVDSV DF7C67111D2386ED mtr1b_human Melatonin receptor type 1B MTNR1B protein protein human Homo sapiens Human MSENGSFANCCEAGGWAVRPGWSGAGSARPSRTPRPPWVAPALSAVLIVTTAVDVVGNLLVILSVLRNRKLRNAGNLFLVSLALADLVVAFYPYPLILVAIFYDGWALGEEHCKASAFVMGLSVIGSVFNITAIAINRYCYICHSMAYHRIYRRWHTPLHICLIWLLTVVALLPNFFVGSLEYDPRIYSCTFIQTASTQYTAAVVVIHFLLPIAVVSFCYLRIWVLVLQARRKAKPESRLCLKPSDLRSFLTMFVVFVIFAICWAPLNCIGLAVAINPQEMAPQIPEGLFVTSYLLAYFNSCLNAIVYGLLNQNFRREYKRILLALWNPRHCIQDASKGSHAEGLQSPAPPIIGVQHQADAL EECD78649DD79121 5ht2c_human 5-hydroxytryptamine receptor 2C 5-hydroxytryptamine receptor 1C Serotonin receptor 2C HTR2C HTR1C protein protein human Homo sapiens Human MVNLRNAVHSFLVHLIGLLVWQCDISVSPVAAIVTDIFNTSDGGRFKFPDGVQNWPALSIVIIIIMTIGGNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDEEKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYCLTIYVLRRQALMLLHGHTEEPPGLSLDFLKCCKRNTAEEENSANPNQDQNARRRKKKERRPRGTMQAINNERKASKVLGIVFFVFLIMWCPFFITNILSVLCEKSCNQKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSNYLRCNYKVEKKPPVRQIPRVAATALSGRELNVNIYRHTNEPVIEKASDNEPGIEMQVENLELPVNPSSVVSERISSV 9E76B3FFD3E09C93 mtnr1a-htr2c-1 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 1A mtnr1b-htr2c-1 1 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 1B htr2c-mtnr1a-1 3 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed c-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 1C htr2c-mtnr1b-1 3 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed P28335:p.Ser138Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S N Tue Jun 30 17:27:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser138Asn' c-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 1C, 5D Figure 5D:- It has been assumed that cells were transfected with MT2-YFP rather than the stated "MT2-Rluc". htr2c-mtnr1b-2 3 6 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed c-terminus renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 1C mtnr1b-htr2c-2 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Figure 1D