IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. FEBS letters 2013 imex curation Wang J., Wang X., Wu G., Hou D., Hu Q. huqi5115@yahoo.cn 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-y79 Homo sapiens y79 y79 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Wang J., Wang X., Wu G., Hou D., Hu Q. The intensity of the EGFP fluorescence was measured instead of the luciferase assay huqi5115@yahoo.cn Accepted 2019-NOV-12 AT 11:20 GMT AT 11:20 GMT by PPORRAS FEBS letters 2013 Only protein-protein interactions imex curation imex curation MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. FEBS letters 2013 imex curation Wang J., Wang X., Wu G., Hou D., Hu Q. huqi5115@yahoo.cn 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-SO-RB50 human SO-RB50 so-rb50 Retinoblastoma sorb50 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Wang J., Wang X., Wu G., Hou D., Hu Q. The intensity of the EGFP fluorescence was measured instead of the luciferase assay huqi5115@yahoo.cn Accepted 2019-NOV-12 AT 11:20 GMT AT 11:20 GMT by PPORRAS FEBS letters 2013 Only protein-protein interactions imex curation imex curation MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6. FEBS letters 2013 imex curation Wang J., Wang X., Wu G., Hou D., Hu Q. huqi5115@yahoo.cn 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-weri-rb1 human weri rb1 weri-rb1 retinoblastoma cell line weri rb1 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Wang J., Wang X., Wu G., Hou D., Hu Q. The intensity of the EGFP fluorescence was measured instead of the luciferase assay huqi5115@yahoo.cn Accepted 2019-NOV-12 AT 11:20 GMT AT 11:20 GMT by PPORRAS FEBS letters 2013 Only protein-protein interactions imex curation imex curation mrna_pax6 paired box 6 mrna messenger rna mRNA mrna human Homo sapiens Human AATATTTTGTGTGAGAGCGAGCGGTGCATTTGCATGTTGCGGAGTGATTAGTGGGTTTGAAAAGGGAACCGTGGCTCGGCCTCATTTCCCGCTCTGGTTCAGGCGCAGGAGGAAGTGTTTTGCTGGAGGATGATGACAGAGGTCAGGCTTCGCTAATGGGCCAGTGAGGAGCGGTGGAGGCGAGGCCGGGCGCCGGCACACACACATTAACACACTTGAGCCATCACCAATCAGCATAGGAATCTGAGAATTGCTCTCACACACCAACCCAGCAACATCCGTGGAGAAAACTCTCACCAGCAACTCCTTTAAAACACCGTCATTTCAAACCATTGTGGTCTTCAAGCAACAACAGCAGCACAAAAAACCCCAACCAAACAAAACTCTTGACAGAAGCTGTGACAACCAGAAAGGATGCCTCATAAAGGGGGAAGACTTTAACTAGGGGCGCGCAGATGTGTGAGGCCTTTTATTGTGAGAGTGGACAGACATCCGAGATTTCAGAGCCCCATATTCGAGCCCCGTGGAATCCCGCGGCCCCCAGCCAGAGCCAGCATGCAGAACAGTCACAGCGGAGTGAATCAGCTCGGTGGTGTCTTTGTCAACGGGCGGCCACTGCCGGACTCCACCCGGCAGAAGATTGTAGAGCTAGCTCACAGCGGGGCCCGGCCGTGCGACATTTCCCGAATTCTGCAGGTGTCCAACGGATGTGTGAGTAAAATTCTGGGCAGGTATTACGAGACTGGCTCCATCAGACCCAGGGCAATCGGTGGTAGTAAACCGAGAGTAGCGACTCCAGAAGTTGTAAGCAAAATAGCCCAGTATAAGCGGGAGTGCCCGTCCATCTTTGCTTGGGAAATCCGAGACAGATTACTGTCCGAGGGGGTCTGTACCAACGATAACATACCAAGCGTGTCATCAATAAACAGAGTTCTTCGCAACCTGGCTAGCGAAAAGCAACAGATGGGCGCAGACGGCATGTATGATAAACTAAGGATGTTGAACGGGCAGACCGGAAGCTGGGGCACCCGCCCTGGTTGGTATCCGGGGACTTCGGTGCCAGGGCAACCTACGCAAGATGGCTGCCAGCAACAGGAAGGAGGGGGAGAGAATACCAACTCCATCAGTTCCAACGGAGAAGATTCAGATGAGGCTCAAATGCGACTTCAGCTGAAGCGGAAGCTGCAAAGAAATAGAACATCCTTTACCCAAGAGCAAATTGAGGCCCTGGAGAAAGAGTTTGAGAGAACCCATTATCCAGATGTGTTTGCCCGAGAAAGACTAGCAGCCAAAATAGATCTACCTGAAGCAAGAATACAGGTATGGTTTTCTAATCGAAGGGCCAAATGGAGAAGAGAAGAAAAACTGAGGAATCAGAGAAGACAGGCCAGCAACACACCTAGTCATATTCCTATCAGCAGTAGTTTCAGCACCAGTGTCTACCAACCAATTCCACAACCCACCACACCGGTTTCCTCCTTCACATCTGGCTCCATGTTGGGCCGAACAGACACAGCCCTCACAAACACCTACAGCGCTCTGCCGCCTATGCCCAGCTTCACCATGGCAAATAACCTGCCTATGCAACCCCCAGTCCCCAGCCAGACCTCCTCATACTCCTGCATGCTGCCCACCAGCCCTTCGGTGAATGGGCGGAGTTATGATACCTACACCCCCCCACATATGCAGACACACATGAACAGTCAGCCAATGGGCACCTCGGGCACCACTTCAACAGGACTCATTTCCCCTGGTGTGTCAGTTCCAGTTCAAGTTCCCGGAAGTGAACCTGATATGTCTCAATACTGGCCAAGATTACAGTAAAAAAAAAAAAAAAAAAAAAAAGGAAAGGAAATATTGTGTTAATTCAGTCAGTGACTATGGGGACACAACAGTTGAGCTTTCAGGAAAGAAAGAAAAATGGCTGTTAGAGCCGCTTCAGTTCTACAATTGTGTCCTGTATTGTACCACTGGGGAAGGAATGGACTTGAAACAAGGACCTTTGTATACAGAAGGCACGATATCAGTTGGAACAAATCTTCATTTTGGTATCCAAACTTTTATTCATTTTGGTGTATTATTTGTAAATGGGCATTTGTATGTTATAATGAAAAAAAGAACAATGTAGACTGGATGGATGTTTGATCTGTGTTGGTCATGAAGTTGTTTTTTTTTTTTTTAAAAAGAAAACCATGATCAACAAGCTTTGCCACGAATTTAAGAGTTTTATCAAGATATATCGAATACTTCTACCCATCTGTTCATAGTTTATGGACTGATGTTCCAAGTTTGTATCATTCCTTTGCATATAATTAAACCTGGAACAACATGCACTAGATTTATGTCAGAAATATCTGTTGGTTTTCCAAAGGTTGTTAACAGATGAAGTTTATGTGCAAAAAAGGGTAAGATATAAATTCAAGGAAGAAAAAAAGTTGATAGCTAAAAGGTAGAGTGTGTCTTCGATATAATCCAATTTGTTTTATGTCAAAATGTAAGTATTTGTCTTCCCTAGAAATCCTCAGAATGATTTCTATAATAAAGTTAATTTCATTTATATTTGACAAGAATATAGATGTTTTATACACATTTTCATGCAATCATACGTTTCTTTTTTGGCCAGCAAAAGTTAATTGTTCTTAGATATAGTTGTATTACTGTTCACGGTCCAATCATTTTGTGCATCTAGAGTTCATTCCTAATCAATTAAAAGTGCTTGCAAGAGTTTTAAACTTAAGTGTTTTGAAGTTGTTCACAACTACATATCAAAATTAACCATTGTTGATTGTAAAAAACCATGCCAAAGCCTTTGTATTTCCTTTATTATACAGTTTTCTTTTTAACCTTATAGTGTGGTGTTACAAATTTTATTTCCATGTTAGATCAACATTCTAAACCAATGGTTACTTTCACACACACTCTGTTTTACATCCTGATGATCCTTAAAAAATAATCCTTATAGATACCATAAATCAAAAACGTGTTAGAAAAAAATTCCACTTACAGCAGGGTGTAGATCTGTGCCCATTTATACCCACAACATATATACAAAATGGTAACATTTCCCAGTTAGCCATTTAATTCTAAAGCTCAAAGTCTAGAAATAATTTAAAAATGCAACAAGCGATTAGCTAGGAATTGTTTTTTGAATTAGGACTGGCATTTTCAATCTGGGCAGATTTCCATTGTCAGCCTATTTCAACAATGATTTCACTGAAGTATATTCAAAAGTAGATTTCTTAAAGGAGACTTTCTGAAAGCTGTTGCCTTTTTCAAATAGGCCCTCTCCCTTTTCTGTCTCCCTCCCCTTTGCACAAGAGGCATCATTTCCCATTGAACCACTACAGCTGTTCCCATTTGAATCTTGCTTTCTGTGCGGTTGTGGATGGTTGGAGGGTGGAGGGGGGATGTTGCATGTCAAGGAATAATGAGCACAGACACATCAACAGACAACAACAAAGCAGACTGTGACTGGCCGGTGGGAATTAAAGGCCTTCAGTCATTGGCAGCTTAAGCCAAACATTCCCAAATCTATGAAGCAGGGCCCATTGTTGGTCAGTTGTTATTTGCAATGAAGCACAGTTCTGATCATGTTTAAAGTGGAGGCACGCAGGGCAGGAGTGCTTGAGCCCAAGCAAAGGATGGAAAAAAATAAGCCTTTGTTGGGTAAAAAAGGACTGTCTGAGACTTTCATTTGTTCTGTGCAACATATAAGTCAATACAGATAAGTCTTCCTCTGCAAACTTCACTAAAAAGCCTGGGGGTTCTGGCAGTCTAGATTAAAATGCTTGCACATGCAGAAACCTCTGGGGACAAAGACACACTTCCACTGAATTATACTCTGCTTTAAAAAAATCCCCAAAAGCAAATGATCAGAAATGTAGAAATTAATGGAAGGATTTAAACATGACCTTCTCGTTCAATATCTACTGTTTTTTAGTTAAGGAATTACTTGTGAACAGATAATTGAGATTCATTGCTCCGGCATGAAATATACTAATAATTTTATTCCACCAGAGTTGCTGCACATTTGGAGACACCTTCCTAAGTTGCAGTTTTTGTATGTGTGCATGTAGTTTTGTTCAGTGTCAGCCTGCACTGCACAGCAGCACATTTCTGCAGGGGAGTGAGCACACATACGCACTGTTGGTACAATTGCCGGTGCAGACATTTCTACCTCCTGACATTTTGCAGCCTACATTCCCTGAGGGCTGTGTGCTGAGGGAACTGTCAGAGAAGGGCTATGTGGGAGTGCATGCCACAGCTGCTGGCTGGCTTACTTCTTCCTTCTCGCTGGCTGTAATTTCCACCACGGTCAGGCAGCCAGTTCCGGCCCACGGTTCTGTTGTGTAGACAGCAGAGACTTTGGAGACCCGGATGTCGCACGCCAGGTGCAAGAGGTGGGAATGGGAGAAAAGGAGTGACGTGGGAGCGGAGGGTCTGTATGTGTGCACTTGGGCACGTATATGTGTGCTCTGAAGGTCAGGATTGCCAGGGCAAAGTAGCACAGTCTGGTATAGTCTGAAGAAGCGGCTGCTCAGCTGCAGAAGCCCTCTGGTCCGGCAGGATGGGAACGGCTGCCTTGCCTTCTGCCCACACCCTAGGGACATGAGCTGTCCTTCCAAACAGAGCTCCAGGCACTCTCTTGGGGACAGCATGGCAGGCTCTGTGTGGTAGCAGTGCCTGGGAGTTGGCCTTTTACTCATTGTTGAAATAATTTTTGTTTATTATTTATTTAACGATACATATATTTATATATTTATCAATGGGGTATCTGCAGGGATGTTTTGACACCATCTTCCAGGATGGAGATTATTTGTGAAGACTTCAGTAGAATCCCAGGACTAAACGTCTAAATTTTTTCTCCAAACTTGACTGACTTGGGAAAACCAGGTGAATAGAATAAGAGCTGAATGTTTTAAGTAATAAACGTTCAAACTGCTCTAAGTAAAAAAATGCATTTTACTGCAATGAATTTCTAGAATATTTTTCCCCCAAAGCTATGCCTCCTAACCCTTAAATGGTGAACAACTGGTTTCTTGCTACAGCTCACTGCCATTTCTTCTTACTATCATCACTAGGTTTCCTAAGATTCACTCATACAGTATTATTTGAAGATTCAGCTTTGTTCTGTGAATGTCATCTTAGGATTGTGTCTATATTCTTTTGCTTATTTCTTTTTACTCTGGGCCTCTCATACTAGTAAGATTTTAAAAAGCCTTTTCTTCTCTGTATGTTTGGCTCACCAAGGCGAAATATATATTCTTCTCTTTTTCATTTCTCAAGAATAAACCTCATCTGCTTTTTTGTTTTTCTGTGTTTTGGCTTGGTACTGAATGACTCAACTGCTCGGTTTTAAAGTTCAAAGTGTAAGTACTTAGGGTTAGTACTGCTTATTTCAATAATGTTGACGGTGACTATCTTTGGAAAGCAGTAACATGCTGTCTTAGAAATGACATTAATAATGGGCTTAAACAAATGAATAGGGGGGTCCCCCCACTCTCCTTTTGTATGCCTATGTGTGTCTGATTTGTTAAAAGATGGACAGGGAATTGATTGCAGAGTGTCGCTTCCTTCTAAAGTAGTTTTATTTTGTCTACTGTTAGTATTTAAAGATCCTGGAGGTGGACATAAGGAATAAATGGAAGAGAAAAGTAGATATTGTATGGTGGCTACTAAAAGGAAATTCAAAAAGTCTTAGAACCCGAGCACCTGAGCAAACTGCAGTAGTCAAAATATTTATCTCATGTTAAAGAAAGGCAAATCTAGTGTAAGAAATGAGTACCATATAGGGTTTTGAAGTTCATATACTAGAAACACTTAAAAGATATCATTTCAGATATTACGTTTGGCATTGTTCTTAAGTATTTATATCTTTGAGTCAAGCTGATAATTAAAAAAAATCTGTTAATGGAGTGTATATTTCATAATGTATCAAAATGGTGTCTATACCTAAGGTAGCATTATTGAAGAGAGATATGTTTATGTAGTAAGTTATTAACATAATGAGTAACAAATAATGTTTCCAGAAGAAAGGAAAACACATTTTCAGAGTGCGTTTTTATCAGAGGAAGACAAAAATACACACCCCTCTCCAGTAGCTTATTTTTACAAAGCCGGCCCAGTGAATTAGAAAAACAAAGCACTTGGATATGATTTTTGGAAAGCCCAGGTACACTTATTATTCAAAATGCACTTTTACTGAGTTTGAAAAGTTTCTTTTATATTTAAAATAAGGGTTCAAATATGCATATTCAATTTTTATAGTAGTTATCTATTTGCAAAGCATATATTAACTAGTAATTGGCTGTTAATTTTATAGACATGGTAGCCAGGGAAGTATATCAATGACCTATTAAGTATTTTGACAAGCAATTTACATATCTGATGACCTCGTATCTCTTTTTCAGCAAGTCAAATGCTATGTAATTGTTCCATTGTGTGTTGTATAAAATGAATCAACACGGTAAGAAAAAGGTTAGAGTTATTAAAATAATAAACTGACTAAAATACTCATTTGAATTTATTCAGAATGTTCATAATGCTTTCAAAGGACATAGCAGAGCTTTTGTGGAGTATCCGCACAACATTATTTATTATCTATGGACTAAATCAATTTTTTGAAGTTGCTTTAAAATTTAAAAGCACCTTTGCTTAATATAAAGCCCTTTAATTTTAACTGACAGATCAATTCTGAAACTTTATTTTGAAAAGAAAATGGGGAAGAATCTGTGTCTTTAGAATTAAAAGAAATGAAAAAAATAAACCCGACATTCTAAAAAAATAGAATAAGAAACCTGATTTTTAGTACTAATGAAATAGCGGGTGACAAAATAGTTGTCTTTTTGATTTTGATCACAAAAAATAAACTGGTAGTGACAGGATATGATGGAGAGATTTGACATCCTGGCAAATCACTGTCATTGATTCAATTATTCTAATTCTGAATAAAAGCTGTATACAGTAAAA hsa-mir-365a-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UAAUGCCCCUAAAAAUCCUUAU hsa_mir_365a 3p-mrna_pax6-1 1 5 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag 3' range 3' range 3' range 3' range r.2090_2096delinscgcctta mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GGGCATT CGCCTTA physical association physical association Fig 2 A, B down-regulates quantity by destabilization hsa_mir_365a 3p-mrna_pax6-2 3 5 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.2090_2096delinscgcctta mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GGGCATT CGCCTTA tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag 3' range 3' range 3' range 3' range physical association physical association Fig 2 A, D down-regulates quantity by destabilization hsa_mir_365a 3p-mrna_pax6-3 2 5 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag 3' range 3' range 3' range 3' range r.2090_2096delinscgcctta mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GGGCATT CGCCTTA physical association physical association Fig 2 A, C down-regulates quantity by destabilization