UniProt UniProt http://www.uniprot.org A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. Cell death & disease 2012 imex curation Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. sha.mi@biogenidec.com 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. No details of protein expression systems given. I have assumed DR6 is purified as a weight is given, so such info for NTR but as they are deriving kinetics from it, and the protein has been labelled, I am also assuming purification. Not an ELISA since neither immune or enzyme-linked components, whatever the authors say. sha.mi@biogenidec.com Accepted 2019-DEC-04 AT 12:56 GMT AT 12:56 GMT by PPORRAS Cell death & disease 2012 Only protein-protein interactions imex curation imex curation A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. Cell death & disease 2012 imex curation Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. sha.mi@biogenidec.com 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-foetal_spinal_cord human foetal spinal cord foetal_spinal_cord Foetal spinal cord anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. sha.mi@biogenidec.com Accepted 2019-DEC-04 AT 12:56 GMT AT 12:56 GMT by PPORRAS Cell death & disease 2012 Only protein-protein interactions imex curation imex curation A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. A DR6/p75(NTR) complex is responsible for β-amyloid-induced cortical neuron death. Cell death & disease 2012 imex curation Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. sha.mi@biogenidec.com 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu Y., Lee X., Shao Z., Apicco D., Huang G., Gong BJ., Pepinsky RB., Mi S. sha.mi@biogenidec.com Accepted 2019-DEC-04 AT 12:56 GMT AT 12:56 GMT by PPORRAS Cell death & disease 2012 Only protein-protein interactions imex curation imex curation tnr21_human Tumor necrosis factor receptor superfamily member 21 TNFRSF21 DR6 Death receptor 6 UNQ437/PRO868 protein protein human Homo sapiens Human MGTSPSSSTALASCSRIARRATATMIAGSLLLLGFLSTTTAQPEQKASNLIGTYRHVDRATGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPVGTFTRHENGIEKCHDCSQPCPWPMIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGWGVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVIKPGTKETDNVCGTLPSFSSSTSPSPGTAIFPRPEHMETHEVPSSTYVPKGMNSTESNSSASVRPKVLSSIQEGTVPDNTSSARGKEDVNKTLPNLQVVNHQQGPHHRHILKLLPSMEATGGEKSSTPIKGPKRGHPRQNLHKHFDINEHLPWMIVLFLLLVLVVIVVCSIRKSSRTLKKGPRQDPSAIVEKAGLKKSMTPTQNREKWIYYCNGHGIDILKLVAAQVGSQWKDIYQFLCNASEREVAAFSNGYTADHERAYAALQHWTIRGPEASLAQLISALRQHRRNDVVEKIRGLMEDTTQLETDKLALPMSPSPLSPSPIPSPNAKLENSALLTVEPSPQDKNKGFFVDESEPLLRCDSTSSGSSALSRNGSFITKEKKDTVLRQVRLDPCDLQPIFDDMLHFLNPEELRVIEEIPQAEDKLDRLFEIIGVKSQEASQTLLDSVYSHLPDLL DIP protein O75509 original sequence version: 118 48939391C4852A33 tnr16_human Tumor necrosis factor receptor superfamily member 16 NGFR TNFRSF16 Low-affinity nerve growth factor receptor Gp80-LNGFR p75 ICD Low affinity neurotrophin receptor p75NTR protein protein human Homo sapiens Human MGAGATGRAMDGPRLLLLLLLGVSLGGAKEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDNLIPVYCSILAAVVVGLVAYIAFKRWNSCKQNKQGANSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQPHTQTASGQALKGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDALLAALRRIQRADLVESLCSESTATSPV DIP protein P08138 original sequence version: 143 B09FA143FB3D625B ngfr-tnfrsf21-1 3 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 2C Second, to determine whether DR6 forms a receptor complex with p75NTR, DR6 was immunoprecipitated from HEK293 cells co-transfected with p75NTR and Myc-tagged DR6. tnfrsf21-ngfr-1 2 4 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 2D Third, we determined if the DR6/p75NTR receptor complex is present endogenously by subjecting human fetal spinal cord lysates to immunoprecipitation with an anti-DR6 antibody followed by western blot analysis to determine if p75NTR co-precipitates with DR6. tnfrsf21-ngfr-2 1 4 unspecified role unspecified role bait bait purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey purified purified region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined europium tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 2F To verify this finding, we directly measured the binding of recombinant DR6 to p75NTR in a competition ELISA (Figure 2f). tnfrsf21-ngfr-3 1 4 competitor competitor competitor prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait purified purified ectodomain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey purified purified region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined europium tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 2F Second, to determine whether DR6 forms a receptor complex with p75NTR, DR6 was immunoprecipitated from HEK293 cells co-transfected with p75NTR and Myc-tagged DR6.