UniProt UniProt http://www.uniprot.org NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. PloS one 2011 imex curation Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. Change to canonical ykanai@pharma1.med.osaka-u.ac.jp Accepted 2020-FEB-10 AT 14:36 GMT AT 14:36 GMT by PPORRAS PloS one 2011 Only protein-protein interactions imex curation imex curation NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. PloS one 2011 imex curation Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp Accepted 2020-FEB-10 AT 14:36 GMT AT 14:36 GMT by PPORRAS PloS one 2011 Only protein-protein interactions imex curation imex curation NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. PloS one 2011 imex curation Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp Accepted 2020-FEB-10 AT 14:36 GMT AT 14:36 GMT by PPORRAS PloS one 2011 Only protein-protein interactions imex curation imex curation NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane. PloS one 2011 imex curation Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. ykanai@pharma1.med.osaka-u.ac.jp 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Hagiwara K., Nagamori S., Umemura YM., Ohgaki R., Tanaka H., Murata D., Nakagomi S., Nomura KH., Kage-Nakadai E., Mitani S., Nomura K., Kanai Y. Change to canonical ykanai@pharma1.med.osaka-u.ac.jp Accepted 2020-FEB-10 AT 14:36 GMT AT 14:36 GMT by PPORRAS PloS one 2011 Only protein-protein interactions imex curation imex curation aat6_caeel Amino acid transporter protein 6 aat-6 T11F9.4 protein protein caeel Caenorhabditis elegans MLNVFGVSASMPDDSRSQKMGLLGAISYIVGNIVGSGIFITPTSIIENVNSVGLSLAIWILAAFISMLGSFCYVELGTSIRLSGGDFAYLCFMKWYPVAFAFMCIGCTINYPATLAVQAQTFAEYVFRGAGVELDETSEFWAKKLLGFSLIILLMFMNFFSLKTFVQRFSILASLAKIAATLLIIITGFYYLIFKHWKQNLEEPFKGSNWNPGPFVNALFAGLFSYDGWDILNFGAEEIENPKRTMPLSIIIGMTCIGVIYVAVNVAYSIVLSPTEMIASNAVAIDFANKTLGAAAFVVPVMVAILLIGSLNSTMFSASRYLQAVSRQGHIPSAISGIAPNCDSPRVALLVHILIAIAVSFLGDPDKLINYVAFAQWSQRAFTMSALLYLRIRGRPRHPDRIQLPIIMPILFFLVCTSMVVISIIDDFKSSAVGLGILLGGLIIFIIFVWDRALPSSHTFRNATHVINEESTKFMQIIFNVVPERVGDEEMKNAIGGAESESEKVPAYKISPTGNGQFKCTRM 7F68479A2F496060 nhrf1_caeel Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MVHIPSDVTPPRLCVVEKLNGENEYGYNLHAEKGRGQFVGTVDPDSPAERGGLITGDRIFAVNGHSIIGENHKKVVERIKANPNRCEMLVISEEGAKWYNENNVQITLDLPNIERVSPMSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM 68FCB98A33515930 g5edm4-1 Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 nrfl-1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MVHIPSDVTPPRLCVVEKLNGENEYGYNLHAEKGRGQFVGTVDPDSPAERGGLITGDRIFAVNGHSIIGENHKKVVERIKANPNRCEMLVISEEGAKWYNENNVQITLDLPNIERVSPMSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM 68FCB98A33515930 g5edm4-2 Na(+)/H(+) exchange regulatory cofactor-like protein nrfl-1 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 nrfl-1 mpz-2 tag-60 C01F6.6 protein protein caeel Caenorhabditis elegans MDRNPSEPERIDWKNPITWISYLYTSFVYYLWLISSHLPHLPQPQRITDDKSTEQITRDAGIPRTEIVTRGDATQTVRQVAGLGDPAPKDSSLLPPSSSFVYCNNCLTPHSSQRTCSPAYVSTLSRQKSLTLLKKLKKPSSSASSTTTTTSSTVSFRAPSRASSTISDASTIRPSELIAIVPSGSHVLLRVPKLDWKTESIRIKRTRMSDWMDAEQRDVMRQFDEVIRSEEEDERTSRKRNGIFKDGSVRSIESLESRSRSKETPVFVPPPPPPTDAMPYLPRLAELNKGTPDQEFGFNLHAERGRGHFIGTVDAGGIGEKAGLEAGQRIVGVNGQLIYPTTGHKEVVALIKKDTMKTTLLVASEDVDKYHKDHNIAYSWDNVERVDTRPVINVETHHHHEEVSVPKSNGYDVPPLNPHSIQVNEEREISKMTTTTRTETITNSNSAYQYKESSTAYDAYATPPVDSNDLMDEVFGRVNLPGVTMSSHTEVLPPTDDISSVSSLSSHRESAVDVPVSHQYVPSYATESHQKHEQHSQTHHHHHQHQQPSPLSNGSSHGYAASSTSGYDDDDIYHLSAREARERLRMKNRKHHLHEMSLNEKYQLVSNM 23E509FA59708DFB aat_6-nrfl_1-1 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Table 1, Fig. 2A aat_6-nrfl_1-2 2 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined G5EDM4-1:p.Gly26_Tyr27delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GY AA Thu Jul 11 17:14:58 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly26Ala;Tyr27Ala]' Tue Jun 30 16:58:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly26_Tyr27delinsAlaAla' G5EDM4-1:p.[Gly26_Tyr27delinsAlaAla;Glu154_Phe155delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GY AA certain certain sequence position certain certain certain sequence position certain EF AA Thu Jul 11 16:48:17 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly26Ala;Tyr27Ala;Glu154Ala;Glu155Ala]]' Tue Jun 30 18:38:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly26_Tyr27delinsAlaAla;Glu154_Phe155delinsAlaAla]' G5EDM4-1:p.Glu154_Phe155delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain EF AA Thu Jul 11 16:45:56 BST 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu154Ala;Glu155Ala]' Tue Jun 30 16:25:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu154_Phe155delinsAlaAla' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2B, 2C aat_6-nrfl_1-3 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Table 1 aat_6-nrfl_1-4 4 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 tag fluorescence tag visualisation by fluorescence tag fluorescence immunohistochemistry immunohistochemistry unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 4B aat_6-nrfl_1-5 1 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 tag fluorescence tag visualisation by fluorescence tag fluorescence western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 4D