IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Biological & pharmaceutical bulletin 2011 imex curation Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. donghak@konkuk.ac.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. The reaction was studied using dilauroylphosphatidylcholine (DLPC) reconstituted systems donghak@konkuk.ac.kr Accepted 2020-JAN-31 AT 08:11 GMT AT 08:11 GMT by MGT Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Biological & pharmaceutical bulletin 2011 imex curation Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. donghak@konkuk.ac.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. donghak@konkuk.ac.kr Accepted 2020-JAN-31 AT 08:11 GMT AT 08:11 GMT by MGT Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Functional characterization of allelic variants of polymorphic human cytochrome P450 2A6 (CYP2A6*5, *7, *8, *18, *19, and *35). Biological & pharmaceutical bulletin 2011 imex curation Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. donghak@konkuk.ac.kr 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro oxidoreduct assay oxidoreductase assay oxidoreduct assay predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. The reaction was studied using dilauroylphosphatidylcholine (DLPC) reconstituted systems NADPH Oxidation Assay (Table 3) was not captured as the participants were not purified donghak@konkuk.ac.kr Accepted 2020-JAN-31 AT 08:11 GMT AT 08:11 GMT by MGT Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation coumarin coumarin 1,2-Benzopyrone 2-Propenoic acid, 3-(2-hydroxyphenyl)-, d-lactone 2-Propenoic acid, 3-(2-hydroxyphenyl)-, delta-lactone 2H-1-Benzopyran-2-one 2H-benzo[b]pyran-2-one 5,6-Benzo-2-pyrone Benzo-a-pyrone Benzo-alpha-pyrone cis-o-Coumarinic acid lactone Coumarine Coumarinic anhydride Cumarin o-hydroxycinnamic acid delta-lactone o-Hydroxycinnamic acid lactone Rattex Tonka bean camphor 2H-chromen-2-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) cp2a6_human Cytochrome P450 2A6 1,4-cineole 2-exo-monooxygenase CYPIIA6 Coumarin 7-hydroxylase Cytochrome P450 IIA3 Cytochrome P450(I) CYP2A6 CYP2A3 protein protein human Homo sapiens Human MLASGMLLVALLVCLTVMVLMSVWQQRKSKGKLPPGPTPLPFIGNYLQLNTEQMYNSLMKISERYGPVFTIHLGPRRVVVLCGHDAVREALVDQAEEFSGRGEQATFDWVFKGYGVVFSNGERAKQLRRFSIATLRDFGVGKRGIEERIQEEAGFLIDALRGTGGANIDPTFFLSRTVSNVISSIVFGDRFDYKDKEFLSLLRMMLGIFQFTSTSTGQLYEMFSSVMKHLPGPQQQAFQLLQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEKNPNTEFYLKNLVMTTLNLFIGGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMPYMEAVIHEIQRFGDVIPMSLARRVKKDTKFRDFFLPKGTEVYPMLGSVLRDPSFFSNPQDFNPQHFLNEKGQFKKSDAFVPFSIGKRNCFGEGLARMELFLFFTTVMQNFRLKSSQSPKDIDVSPKHVGFATIPRNYTMSFLPR 8C05CBF0EFC698AF nicotine nicotine (+-)-nicotine (+-)-3-(1-Methyl-2-pyrrolidinyl)pyridine (R,S)-nicotine (RS)-nicotine Nicotin Nikotin 3-(1-methylpyrrolidin-2-yl)pyridine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) coumarin-cyp2a6-1 2 5 unspecified role unspecified role neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 17:45:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile471Thr' P11509:p.Arg485Leu CYP2A6*8 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 18:50:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg485Leu' P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:04:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr392Phe' P11509:p.Asn438Tyr CYP2A6*35 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Jun 30 17:35:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn438Tyr' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 19:01:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr392Phe;Ile471Thr]' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, Figure 2 coumarin-cyp2a6-2 1 4 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 17:33:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile471Thr' P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:02:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr392Phe' P11509:p.Arg485Leu CYP2A6*8 mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 20:24:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg485Leu' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 16:50:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr392Phe;Ile471Thr]' P11509:p.Asn438Tyr mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain N Y Tue Jun 30 18:36:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn438Tyr' ecoli Escherichia coli (strain K12) hydroxylation hydroxylation reaction hydroxylation Table 2 cyp2a6-nicotine-1 3 6 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 17:40:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile471Thr' P11509:p.Arg485Leu CYP2A6*8 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R L Tue Jun 30 19:52:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg485Leu' P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 15:44:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr392Phe' P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain I T Tue Jun 30 16:38:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr392Phe;Ile471Thr]' P11509:p.Asn438Tyr CYP2A6*35 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain N Y Tue Jun 30 20:18:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn438Tyr' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) redox reaction oxidoreductase activity electron transfer reaction redox reaction Table 2, Figure 3