UniProt UniProt http://www.uniprot.org Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Journal of leukocyte biology 2012 imex curation Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu Accepted 2019-DEC-30 AT 08:45 GMT AT 08:45 GMT by MGT Journal of leukocyte biology 2012 Only protein-protein interactions imex curation imex curation Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Journal of leukocyte biology 2012 imex curation Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u373 Homo sapiens u373 u373 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu Accepted 2019-DEC-30 AT 08:45 GMT AT 08:45 GMT by MGT Journal of leukocyte biology 2012 Only protein-protein interactions imex curation imex curation Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Serine phosphorylation of FcγRI cytoplasmic domain directs lipid raft localization and interaction with protein 4.1G. Journal of leukocyte biology 2012 imex curation Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gibson AW., Li X., Wu J., Baskin JG., Raman C., Edberg JC., Kimberly RP. gibsona@uab.edu Accepted 2019-DEC-30 AT 08:45 GMT AT 08:45 GMT by MGT Journal of leukocyte biology 2012 Only protein-protein interactions imex curation imex curation e41l2_human Band 4.1-like protein 2 Generally expressed protein 4.1 EPB41L2 Erythrocyte membrane protein band 4.1-like 2 protein protein human Homo sapiens Human MTTEVGSVSEVKKDSSQLGTDATKEKPKEVAENQQNQSSDPEEEKGSQPPPAAESQSSLRRQKREKETSESRGISRFIPPWLKKQKSYTLVVAKDGGDKKEPTQAVVEEQVLDKEEPLPEEQRQAKGDAEEMAQKKQEIKVEVKEEKPSVSKEEKPSVSKVEMQPTELVSKEREEKVKETQEDKLEGGAAKRETKEVQTNELKAEKASQKVTKKTKTVQCKVTLLDGTEYSCDLEKHAKGQVLFDKVCEHLNLLEKDYFGLLFQESPEQKNWLDPAKEIKRQLRNLPWLFTFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHALLGSYTLQAELGDYDPEEHGSIDLSEFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPHFERTSSKRVSRSLDGAPIGVMDQSLMKDFPGAAGEISAYGPGLVSIAVVQDGDGRREVRSPTKAPHLQLIEGKKNSLRVEGDNIYVRHSNLMLEELDKAQEDILKHQASISELKRNFMESTPEPRPNEWEKRRITPLSLQTQGSSHETLNIVEEKKRAEVGKDERVITEEMNGKEISPGSGPGEIRKVEPVTQKDSTSLSSESSSSSSESEEEDVGEYRPHHRVTEGTIREEQEYEEEVEEEPRPAAKVVEREEAVPEASPVTQAGASVITVETVIQENVGAQKIPGEKSVHEGALKQDMGEEAEEEPQKVNGEVSHVDIDVLPQIICCSEPPVVKTEMVTISDASQRTEISTKEVPIVQTETKTITYESPQIDGGAGGDSGTLLTAQTITSESVSTTTTTHITKTVKGGISETRIEKRIVITGDGDIDHDQALAQAIREAREQHPDMSVTRVVVHKETELAEEGED E86CB17488F6045F fcgr1_human High affinity immunoglobulin gamma Fc receptor I FCGR1A FCG1 FCGR1 IGFR1 Fc-gamma RI Fc-gamma RIA protein protein human Homo sapiens Human MWFLTTLLLWVPVDGQVDTTKAVITLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPTPVWFHVLFYLAVGIMFLVNTVLWVTIRKELKRKKKWDLEISLDSGHEKKVISSLQEDRHLEEELKCQEQKEEQLQEGVHRKEPQGAT D33D59398CEEA699 fcgr1a-epb41l2-1 1 5 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytoplasmic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined increasing-ptm ptm increasing an interaction increasing-ptm ecoli Escherichia coli (strain K12) physical association physical association Fig. 1A, 2C GST-FcγRI CY fusion protein, but not GST alone, was able to pull down a protein 4.1G-ThioHis fusion from bacterial lysate (Fig. 1A, lane 4 vs. lane 2). Immobilized GST-FcγRI cytoplasmic domain was treated with Casein Kinase 2 complex and ATP before incubation with protein 4.1G fcgr1a-epb41l2-2 3 5 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 1B FcγRI CY coprecipitates with protein 4.1G in human PBMCs fcgr1a-epb41l2-3 2 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Fig. 1C FcγRI CY coprecipitates with protein 4.1G in human PBMCs