IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae. Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae. Acta crystallographica. Section D, Biological crystallography 2011 imex curation French JB., Ealick SE. see3@cornell.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined French JB., Ealick SE. see3@cornell.edu Accepted 2020-MAR-11 AT 09:01 GMT AT 09:01 GMT by PPORRAS Acta crystallographica. Section D, Biological crystallography 2011 Only protein-protein interactions imex curation imex curation Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae. Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae. Acta crystallographica. Section D, Biological crystallography 2011 imex curation French JB., Ealick SE. see3@cornell.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined French JB., Ealick SE. see3@cornell.edu Accepted 2020-MAR-11 AT 09:01 GMT AT 09:01 GMT by PPORRAS Acta crystallographica. Section D, Biological crystallography 2011 Only protein-protein interactions imex curation imex curation 5-hydroxyisouric acid 5-hydroxyisouric acid 5-Hydroxyisourate 5-hydroxyisourate 5-hydroxy-5,7-dihydro-1H-purine-2,6,8(9H)-trione small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) a6t926_klep7 5-hydroxyisourate hydrolase KPN_01666 protein protein klep7 Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) MSTLSTHILDISTGTPAEGVTVSLSREGETLANLVTNAQGRIATFSAAPLPAGRYCLTAETGAWFARAGRESVFTRAQIDFVIGEAAEDHFHLPFLIAPGGWSTYRGS C480AD3D7BE3C95B a6t926_klep7-1 1 4 unspecified role unspecified role neutral component neutral component purified purified Full length protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Fig.2, table 1 1.76A Rwork=19.8 Rfree=22.2 a6t926_klep7-2 2 3 enzyme target enzyme target substrate substrate neutral component neutral component 4 enzyme enzyme neutral component neutral component full length protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain A6T926:p.His7Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H N Tue Jun 30 18:36:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His7Asn' A6T926:p.Arg41Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R K Tue Jun 30 16:55:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg41Lys' A6T926:p.His92Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H N Tue Jun 30 18:42:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His92Asn' A6T926:p.Ser108Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:42:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser108Ala' ecobd Escherichia coli (strain B / BL21-DE3) hydroxylation hydroxylation reaction hydroxylation Fig.1, Table 2