IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. The Journal of cell biology 2010 imex curation Garbett D., LaLonde DP., Bretscher A. apb5@cornell.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-jeg3 Homo sapiens choriocarcinoma JEG3 jeg3 Human choriocarcinoma JEG3 confocal microscopy confocal microscopy immunostaining immunostaining Garbett D., LaLonde DP., Bretscher A. PABLO: Forgot to mention fig 10B in the list of figures containing this colocalization, please add that one too. apb5@cornell.edu Accepted 2019-SEP-25 AT 14:20 BST AT 14:20 BST by PPORRAS The Journal of cell biology 2010 Only protein-protein interactions imex curation imex curation The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. The Journal of cell biology 2010 imex curation Garbett D., LaLonde DP., Bretscher A. apb5@cornell.edu 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Garbett D., LaLonde DP., Bretscher A. Source of 6xHis-EPI64 construct gifted by Dr.Janet Ingraffea was found to be human (PMID: 22219378); source of beta-catenin was not provided anywhere in the reference and hence Figure 9c was not captured apb5@cornell.edu Accepted 2019-SEP-25 AT 14:20 BST AT 14:20 BST by PPORRAS The Journal of cell biology 2010 Only protein-protein interactions imex curation imex curation nhrf1_human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein human Homo sapiens Human MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL E33AF87016D37A65 tb10a_human TBC1 domain family member 10A TBC1D10A EPI64 TBC1D10 Rab27A-GAP-alpha EBP50-PDX interactor of 64 kDa protein protein human Homo sapiens Human MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDELDMSPGDPKWLDVIERDLHRQFPFHEMFVSRGGHGQQDLFRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEQAFWCLVQICEKYLPGYYSEKLEAIQLDGEILFSLLQKVSPVAHKHLSRQKIDPLLYMTEWFMCAFSRTLPWSSVLRVWDMFFCEGVKIIFRVGLVLLKHALGSPEKVKACQGQYETIERLRSLSPKIMQEAFLVQEVVELPVTERQIEREHLIQLRRWQETRGELQCRSPPRLHGAKAILDAEPGPRPALQPSPSIRLPLDAPLPGSKAKPKPPKQAQKEQRKQMKGRGQLEKPPAPNQAMVVAAAGDACPPQHVPPKDSAPKDSAPQDLAPQVSAHHRSQESLTSQESEDTYL mint 17BFAA85BE86DE84 ezri_human Ezrin EZR VIL2 p81 Cytovillin Villin-2 protein protein human Homo sapiens Human MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL DIP protein P15311 original sequence version: 170 DIP protein P15311 original sequence version: 142 F1B592CF49A7CC46 ezr-slc9a3r1-1 2 5 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey purified purified region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O14745:p.Phe355Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F A Tue Jun 30 16:15:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe355Ala' O14745:p.Leu358delinsLeuAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L LA Tue Jun 30 20:15:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu358delinsLeuAla' p.Phe355_Leu358del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain FSNL . O14745:p.[Met346Ala;Trp348Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 19:30:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met346Ala;Trp348Ala]' O14745:p.Phe355Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F R Tue Jun 30 16:47:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe355Arg' O14745:p.Leu358del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L . Tue Jun 30 19:43:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu358del' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-jeg3 Homo sapiens choriocarcinoma JEG3 jeg3 Human choriocarcinoma JEG3 direct interaction direct interaction Figure 2b The EBP50 (SLC9A3R1)-ezrin interaction is critical for microvilli; Mutations in EBP50 (SLC9A3R1) - p.Phe355_Leu358del, p.[Met346Ala;Trp348Ala], p.Phe355Arg, p.Leu358del mutations abolish microvilli; p.Phe355Ala was not able to restore microvilli; p.Leu358delinsLeuAla was fully competent to form microvilli ezr-slc9a3r1-2 1 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O14745:p.Leu358delinsLeuAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L LA Tue Jun 30 20:17:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu358delinsLeuAla' O14745:p.Leu358del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L . Tue Jun 30 15:43:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu358del' O14745:p.[Ser280Ala;Ser302Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:28:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Ala;Ser302Ala]' O14745:p.[Ser280Asp;Ser302Asp] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 17:13:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Asp;Ser302Asp]' O14745:p.[Ser162Ala;Ser339Ala;Ser340Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:35:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Ala;Ser339Ala;Ser340Ala]' O14745:p.[Ser162Asp;Ser339Asp;Ser340Asp] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 19:04:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Asp;Ser339Asp;Ser340Asp]' EBP50 (SIC9A3R1) was rendered siRNA resistance by introduction of mutations without affecting the protein sequence colocalization colocalization Figure 3b, 4b, 5b, 6b, 7b, 10b, S3b Mutations of EBP50 S162,339,340A and S162,339,340D were not captured as there are two substitution occurring at the same position and cannot be to fit in our feature slc9a3r1-tbc1d10a-1 2 4 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey purified purified O14745:p.[Ser280Ala;Ser302Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:53:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Ala;Ser302Ala]' O14745:p.[Ser280Asp;Ser302Asp] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 20:22:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Asp;Ser302Asp]' O14745:p.[Ser162Ala;Ser339Ala;Ser340Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 17:44:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Ala;Ser339Ala;Ser340Ala]' O14745:p.[Ser162Asp;Ser339Asp;Ser340Asp] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 16:52:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Asp;Ser339Asp;Ser340Asp]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figure 9a ezr-slc9a3r1-3 2 4 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey purified purified O14745:p.[Ser280Ala;Ser302Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:24:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Ala;Ser302Ala]' O14745:p.[Ser280Asp;Ser302Asp] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 16:57:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser280Asp;Ser302Asp]' O14745:p.[Ser162Ala;Ser339Ala;Ser340Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:04:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Ala;Ser339Ala;Ser340Ala]' O14745:p.[Ser162Asp;Ser339Asp;Ser340Asp] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 20:22:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser162Asp;Ser339Asp;Ser340Asp]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Figure 9b