DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Novel protein-protein interactions inferred from literature context. Novel protein-protein interactions inferred from literature context. PloS one 2008 imex curation van Haagen HH., 't Hoen PA., Botelho Bovo A., de Morrée A., van Mulligen EM., Chichester C., Kors JA., den Dunnen JT., van Ommen GJ., van der Maarel SM., Kern VM., Mons B., Schuemie MJ. hvanhaagen@lumc.nl 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp van Haagen HH., 't Hoen PA., Botelho Bovo A., de Morrée A., van Mulligen EM., Chichester C., Kors JA., den Dunnen JT., van Ommen GJ., van der Maarel SM., Kern VM., Mons B., Schuemie MJ. hvanhaagen@lumc.nl Accepted 2019-SEP-30 AT 14:01 BST AT 14:01 BST by MGT PloS one 2008 Only protein-protein interactions imex curation imex curation parvb_mouse Beta-parvin Parvb protein protein mouse Mus musculus Mouse MSSAPPRSPTPRAPKMKKDESFLGKLGGTLARKKKTREVTDLQEEGKSAINSPMAPALVDIHPEDTQLEENEERTMIDPTSREDPKFKELVKVLLDWINDVLAEERIIVKQLEEDLYDGQVLQKLLEKLAHCKLNVAEVTQSEIGQKQKLQTVLEAVQDLLRPHGWPLRWNVDSIHGKNLVAILHLLVSLAMHFRAPIHLPEHVTVQVVVVRKREGLLHSSHISEELTTTTEIMMGRFERDAFDTLFDHAPDKLNLVKKSLITFVNKHLNKLNLEVTDLETQFADGVYLVLLLGLLEDYFVPLHNFYLTPDSFDQKVHNVAFAFELMLDGGLKKPKARPEDVVNLDLKSTLRVLYTLFTKYKDVE DIP protein Q9ES46 original sequence version: 74 A3C6B8B3D5875CEA can3_mouse Calpain-3 Calcium-activated neutral proteinase 3 Calpain L3 Calpain p94 Muscle-specific calcium-activated neutral protease 3 Capn3 protein protein mouse Mus musculus Mouse MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPSGIYSAIISRNFPIIGVKEKTFEQLRRKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRGSDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEGKDKAGPEKRGETPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA C7FC94E31E1084C9 parvb-capn3-3 1 2 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-term range C-terminal range c-term range c-term range C-terminal range c-term range gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-IM2 IM2 primary myoblast cell line im2 IM2 primary myoblast cell line 3 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-IM2 IM2 primary myoblast cell line im2 IM2 primary myoblast cell line physical association physical association Figure S2C lane 1 parvb-capn3-2 1 2 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-IM2 IM2 primary myoblast cell line im2 IM2 primary myoblast cell line 3 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Exon 6 deletion Exon 6 deletion sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse-IM2 IM2 primary myoblast cell line im2 IM2 primary myoblast cell line physical association physical association Supplementary Figure 2D parvb-capn3-4 1 2 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-term range n-terminal range n-term range n-term range n-terminal range n-term range gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-term range n-terminal range n-term range n-term range n-terminal range n-term range t7 tag t7 tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q64691:p.Cys129Ser C129S mutation mutation certain certain sequence position certain certain certain sequence position certain C S Tue Jun 30 16:59:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys129Ser' ecoli Escherichia coli (strain K12) physical association physical association Figure S2A, Lane 2