IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. The Journal of cell biology 2007 imex curation Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu Accepted 2019-JUN-28 AT 07:52 BST AT 07:52 BST by ORCHARD The Journal of cell biology 2007 Only protein-protein interactions imex curation imex curation All done, but expressed in Hi-5 cells instead of Ecoli as for M&M ;-) SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. The Journal of cell biology 2007 imex curation Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu Accepted 2019-JUN-28 AT 07:52 BST AT 07:52 BST by ORCHARD The Journal of cell biology 2007 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. The Journal of cell biology 2007 imex curation Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu Accepted 2019-JUN-28 AT 07:52 BST AT 07:52 BST by ORCHARD The Journal of cell biology 2007 Only protein-protein interactions imex curation imex curation All done, but expressed in Hi-5 cells instead of Ecoli as for M&M ;-) SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. The Journal of cell biology 2007 imex curation Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu Accepted 2019-JUN-28 AT 07:52 BST AT 07:52 BST by ORCHARD The Journal of cell biology 2007 Only protein-protein interactions imex curation imex curation SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression. The Journal of cell biology 2007 imex curation Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Tsang WY., Wang L., Chen Z., Sánchez I., Dynlacht BD. From introduction: Cyclin A corresponds to CyclinA2. From PMID: 8491188 GST-CyclinA is human and c-terminally tagged. From PMID: 1535244 cdk2 is human. Fig. 1A-C, kinase assay shown in Fig. 4D and interactions detected using aspecific antibodies were not captured since not compliant to IMEx. brian.dynlacht@med.nyu.edu Accepted 2019-JUN-28 AT 07:52 BST AT 07:52 BST by ORCHARD The Journal of cell biology 2007 Only protein-protein interactions imex curation imex curation All done, but expressed in Hi-5 cells instead of Ecoli as for M&M ;-) scape_human S phase cyclin A-associated protein in the endoplasmic reticulum SCAPER KIAA1454 ZNF291 Zinc finger protein 291 MSTP063 protein protein human Homo sapiens Human MMASFQRSNSHDKVRRIVAEEGRTARNLIAWSVPLESKDDDGKPKCQTGGKSKRTIQGTHKTTKQSTAVDCKITSSTTGDKHFDKSPTKTRHPRKIDLRARYWAFLFDNLRRAVDEIYVTCESDQSVVECKEVLMMLDNYVRDFKALIDWIQLQEKLEKTDAQSRPTSLAWEVKKMSPGRHVIPSPSTDRINVTSNARRSLNFGGSTGTVPAPRLAPTGVSWADKVKAHHTGSTASSEITPAQSCPPMTVQKASRKNERKDAEGWETVQRGRPIRSRSTAVMPKVSLATEATRSKDDSDKENVCLLPDESIQKGQFVGDGTSNTIESHPKDSLHSCDHPLAEKTQFTVSTLDDVKNSGSIRDNYVRTSEISAVHIDTECVSVMLQAGTPPLQVNEEKFPAEKARIENEMDPSDISNSMAEVLAKKEELADRLEKANEEAIASAIAEEEQLTREIEAEENNDINIETDNDSDFSASMGSGSVSFCGMSMDWNDVLADYEARESWRQNTSWGDIVEEEPARPPGHGIHMHEKLSSPSRKRTIAESKKKHEEKQMKAQQLREKLREEKTLKLQKLLEREKDVRKWKEELLDQRRRMMEEKLLHAEFKREVQLQAIVKKAQEEEAKVNEIAFINTLEAQNKRHDVLSKLKEYEQRLNELQEERQRRQEEKQARDEAVQERKRALEAERQARVEELLMKRKEQEARIEQQRQEKEKAREDAARERARDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAELSSGRHANTDYAPKLTPYERKKQCSLCNVLISSEVYLFSHVKGRKHQQAVRENTSIQGRELSDEEVEHLSLKKYIIDIVVESTAPAEALKDGEERQKNKKKAKKIKARMNFRAKEYESLMETKNSGSDSPYKAKLQRLAKDLLKQVQVQDSGSWANNKVSALDRTLGEITRILEKENVADQIAFQAAGGLTALEHILQAVVPATNVNTVLRIPPKSLCNAINVYNLTCNNCSENCSDVLFSNKITFLMDLLIHQLTVYVPDENNTILGRNTNKQVFEGLTTGLLKVSAVVLGCLIANRPDGNCQPATPKIPTQEMKNKPSQGDPFNNRVQDLISYVVNMGLIDKLCACFLSVQGPVDENPKMAIFLQHAAGLLHAMCTLCFAVTGRSYSIFDNNRQDPTGLTAALQATDLAGVLHMLYCVLFHGTILDPSTASPKENYTQNTIQVAIQSLRFFNSFAALHLPAFQSIVGAEGLSLAFRHMASSLLGHCSQVSCESLLHEVIVCVGYFTVNHPDNQVIVQSGRHPTVLQKLCQLPFQYFSDPRLIKVLFPSLIAACYNNHQNKIILEQEMSCVLLATFIQDLAQTPGQAENQPYQPKGKCLGSQDYLELANRFPQQAWEEARQFFLKKEKK 9DBAF02F75CB1AA7 ccna2_human Cyclin-A2 Cyclin A CCNA2 CCN1 CCNA protein protein human Homo sapiens Human MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL DIP protein P20248 original sequence version: 183 DIP protein P20248 original sequence version: 146 DIP protein P20248 original sequence version: 168 DIP protein P20248 original sequence version: 149 DIP protein P20248 original sequence version: 111 97763A2897DD35F4 cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP DIP protein P38936 original sequence version: 120 DIP protein P38936 original sequence version: 165 DIP protein P38936 original sequence version: 160 98D1E7C519ADFCA9 cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 cyclina_cdk2-1 6 7 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 15 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 17 trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association na To identify p158/p160, we performed a large-scale purification of the protein from human cell extracts using resin containing recombinant GST-cyclin A/Cdk2 protein. The 158/160-kD band was subjected to mass spectrometric sequencing. This analysis revealed that a large number of peptides belonged to two uncharacterized proteins with similar molecular masses. One of these polypeptides has a theoretical molecular weight of 158 kD and encoded a putative zinc finger motif (sequenced previously as a cDNA designated ZNF291, GenBank/EMBL/DDBJ accession no. AF242528; Fig. 1 D). cyca-1 5 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 3A Also cyclinA and E were detected. cdkn1a-cdk2-9 1 10 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 3C Also cyclinA was detected. cdkn1a-cdk2-10 1 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 3C Also cyclinA was detected. cdk2-scaper-1 4 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BY12:p.Arg26_Leu28delinsAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RNL AAA Tue Jun 30 16:21:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg26_Leu28delinsAlaAlaAla' Q9BY12:p.Arg199_Leu201delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RSL AAA Tue Jun 30 18:38:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg199_Leu201delinsAlaAlaAla' Q9BY12:p.Arg678_Leu680delinsAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RAL AAA Tue Jun 30 15:38:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg678_Leu680delinsAlaAlaAla' physical association physical association Fig. 4B cyclin A was also immunoprecipitated scaper-cdk2-1 2 8 unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 11 unspecified role unspecified role prey prey purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 4C cyclina2-cdk2_human Cyclin A2: CDK2 Cyclin A2-CDK2 complex CCNA2:CDK2 8 unspecified role unspecified role cdk2 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 11 enzyme enzyme ccna2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 44 46 direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cyclin-dependent protein kinase complex. Required in G1 phase of cell cycle. Complex formation enables substrate binding to the kinase, leads to a rearrangement of the catalytic site and exposes Thr-160 of CDK2 for phosphorylation and subsequent activation. Heterodimer created with intact-jami-dbscripts