IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. Molecular cell 2007 imex curation Wang J., Hu W., Cai S., Lee B., Song J., Chen Y. ychen@coh.org 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wang J., Hu W., Cai S., Lee B., Song J., Chen Y. ychen@coh.org Accepted 2019-MAY-24 AT 11:42 BST AT 11:42 BST by LIVIAP Molecular cell 2007 Only protein-protein interactions imex curation imex curation Supplementary figure legends added to EBI-21399662; Two separate interactions created The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. Molecular cell 2007 imex curation Wang J., Hu W., Cai S., Lee B., Song J., Chen Y. ychen@coh.org 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro sumoylase assay sumoylase assay weight by staining molecular weight estimation by staining weight by staining Wang J., Hu W., Cai S., Lee B., Song J., Chen Y. ychen@coh.org Accepted 2019-MAY-24 AT 11:42 BST AT 11:42 BST by LIVIAP Molecular cell 2007 Only protein-protein interactions imex curation imex curation Supplementary figure legends added to EBI-21399662; Two separate interactions created sae2_human SUMO-activating enzyme subunit 2 Ubiquitin-like modifier-activating enzyme 2 HRIHFB2115 Ubiquitin-like 1-activating enzyme E1B Anthracycline-associated resistance ARX UBA2 SAE2 UBLE1B protein protein human Homo sapiens Human MALSRGLPRELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDWAEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD DIP protein Q9UBT2 original sequence version: 85 C12D15293BBF90EB ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63279 original sequence version: 33 DIP protein P63279 original sequence version: 57 DIP protein P63279 original sequence version: 80 DIP protein P63279 original sequence version: 137 DIP protein P63279 original sequence version: 99 DIP protein P63279 original sequence version: 140 DIP protein P63279 original sequence version: 120 DIP protein P63279 original sequence version: 117 DIP protein P63279 original sequence version: 93 E2C826E9C8D0683D uba2-ube2i-1 1 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 1b uba2-ube2i-2 1 3 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified P63279:p.Cys138Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 19:58:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys138Ala' P63279:p.Cys93Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 19:54:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ala' region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 1c, 2a, 2b, S1 uba2-ube2i-3 2 3 enzyme enzyme neutral component neutral component purified purified region sumoylated lysine sumoylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate neutral component neutral component purified purified P63279:p.Glu132Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 18:46:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu132Ala' P63279:p.Gln130Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 19:17:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln130Ala' region sumoylated lysine sumoylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm P63279:p.Tyr134Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 15:54:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr134Ala' P63279:p.Ala131Asp mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain A D Tue Jun 30 18:00:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala131Asp' ecoli Escherichia coli (strain K12) sumoylation sumoylation reaction sumoylation Figure 4a, 4d uba2-ube2i-4 2 3 enzyme enzyme neutral component neutral component purified purified Q9UBT2:p.[Ile235Ala;Ile238Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:48:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile235Ala;Ile238Ala]' region sumoylated lysine sumoylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region sumoylated lysine sumoylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm ecoli Escherichia coli (strain K12) sumoylation sumoylation reaction sumoylation Figure 4f