IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mutations in VANGL1 associated with neural-tube defects. Mutations in VANGL1 associated with neural-tube defects. The New England journal of medicine 2007 imex curation Kibar Z., Torban E., McDearmid JR., Reynolds A., Berghout J., Mathieu M., Kirillova I., De Marco P., Merello E., Hayes JM., Wallingford JB., Drapeau P., Capra V., Gros P. philippe.gros@mcgill.ca 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kibar Z., Torban E., McDearmid JR., Reynolds A., Berghout J., Mathieu M., Kirillova I., De Marco P., Merello E., Hayes JM., Wallingford JB., Drapeau P., Capra V., Gros P. Dvl1, Dvl2 and Dvl3 constructs were found to be of mouse origin (pmid: 15456783) philippe.gros@mcgill.ca Accepted 2019-SEP-16 AT 15:36 BST AT 15:36 BST by LUANA The New England journal of medicine 2007 Only protein-protein interactions imex curation imex curation dvl1_mouse Segment polarity protein dishevelled homolog DVL-1 Dvl1 Dvl DSH homolog 1 protein protein mouse Mus musculus Mouse MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNRDEAARTNGHPRGDRRRDLGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQSTSSRLVRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFSEQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSCGSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSPRSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM DIP protein P51141 original sequence version: 147 FA21ACAC48FF71E0 vang1_human Vang-like protein 1 Van Gogh-like protein 1 Strabismus 2 Loop-tail protein 2 homolog VANGL1 STB2 protein protein human Homo sapiens Human MDTESTYSGYSYYSSHSKKSHRQGERTRERHKSPRNKDGRGSEKSVTIQPPTGEPLLGNDSTRTEEVQDDNWGETTTAITGTSEHSISQEDIARISKDMEDSVGLDCKRYLGLTVASFLGLLVFLTPIAFILLPPILWRDELEPCGTICEGLFISMAFKLLILLIGTWALFFRKRRADMPRVFVFRALLLVLIFLFVVSYWLFYGVRILDSRDRNYQGIVQYAVSLVDALLFIHYLAIVLLELRQLQPMFTLQVVRSTDGESRFYSLGHLSIQRAALVVLENYYKDFTIYNPNLLTASKFRAAKHMAGLKVYNVDGPSNNATGQSRAMIAAAARRRDSSHNELYYEEAEHERRVKKRKARLVVAVEEAFIHIQRLQAEEQQKAPGEVMDPREAAQAIFPSMARALQKYLRITRQQNYHSMESILQHLAFCITNGMTPKAFLERYLSAGPTLQYDKDRWLSTQWRLVSDEAVTNGLRDGIVFVLKCLDFSLVVNVKKIPFIILSEEFIDPKSHKFVLRLQSETSV 65CB263D26274585 dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein Q60838 original sequence version: 132 DIP protein Q60838 original sequence version: 63 DIP protein Q60838 original sequence version: 125 DIP protein Q60838 original sequence version: 69 DIP protein Q60838 original sequence version: 91 928A527143BBBBB0 dvl3_mouse Segment polarity protein dishevelled homolog DVL-3 Dvl3 DSH homolog 3 protein protein mouse Mus musculus Mouse MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFHPHASGGSQENLDNDTETDSLVSAQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM 552A5BE4BEA801F0 dvl1-vangl1-1 1 2 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region gal4 dna bd gal4 dna binding domain gal4 dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8TAA9:p.Val239Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V I Tue Jun 30 16:38:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val239Ile' Q8TAA9:p.Asp259Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D E Tue Jun 30 18:47:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp259Glu' Q8TAA9:p.Arg274Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 15:44:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg274Gln' Q8TAA9:p.Met328Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M T Tue Jun 30 19:26:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met328Thr' Q8TAA9:p.Ser467Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S N Tue Jun 30 17:45:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser467Asn' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2b Interaction studied in the most stringent medium which supports the growth of diploid cells only if proteins interact (labeled –Ade/–His/–Leu/–Trp lacking adenine, histidine, leucine, and tryptophan) was curated vangl1-dvl1-2 1 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region gal4 dna bd gal4 dna binding domain gal4 dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8TAA9:p.Val239Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V I Tue Jun 30 18:44:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val239Ile' Q8TAA9:p.Asp259Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D E Tue Jun 30 18:20:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp259Glu' Q8TAA9:p.Arg274Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 17:44:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg274Gln' Q8TAA9:p.Met328Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M T Tue Jun 30 16:30:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met328Thr' Q8TAA9:p.Ser467Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S N Tue Jun 30 16:41:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser467Asn' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2b Interaction studied in the most stringent medium which supports the growth of diploid cells only if proteins interact (labeled –Ade/–His/–Leu/–Trp lacking adenine, histidine, leucine, and tryptophan) was curated vangl1-dvl2-4 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gal4 ad gal4 activation domain gal4 ad undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region gal4 dna bd gal4 dna binding domain gal4 dna bd undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8TAA9:p.Val239Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V I Tue Jun 30 19:27:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val239Ile' Q8TAA9:p.Asp259Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D E Tue Jun 30 19:33:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp259Glu' Q8TAA9:p.Arg274Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 18:16:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg274Gln' Q8TAA9:p.Met328Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M T Tue Jun 30 20:01:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met328Thr' Q8TAA9:p.Ser467Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S N Tue Jun 30 17:20:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser467Asn' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2b Interaction studied in the most stringent medium which supports the growth of diploid cells only if proteins interact (labeled –Ade/–His/–Leu/–Trp lacking adenine, histidine, leucine, and tryptophan) was curated