IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional significance and clinical phenotype of nontruncating mismatch repair variants of MLH1. Functional significance and clinical phenotype of nontruncating mismatch repair variants of MLH1. Gastroenterology 2005 imex curation Raevaara TE., Korhonen MK., Lohi H., Hampel H., Lynch E., Lönnqvist KE., Holinski-Feder E., Sutter C., McKinnon W., Duraisamy S., Gerdes AM., Peltomäki P., Kohonen-Ccorish M., Mangold E., Macrae F., Greenblatt M., de la Chapelle A., Nyström M. minna.nystrom@helsinki.fi. 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Raevaara TE., Korhonen MK., Lohi H., Hampel H., Lynch E., Lönnqvist KE., Holinski-Feder E., Sutter C., McKinnon W., Duraisamy S., Gerdes AM., Peltomäki P., Kohonen-Ccorish M., Mangold E., Macrae F., Greenblatt M., de la Chapelle A., Nyström M. MHL1 and PMS2 constructs were found to be of human origin (12362032 & 12891553 respectively) minna.nystrom@helsinki.fi. Accepted 2019-SEP-18 AT 08:35 BST AT 08:35 BST by PPORRAS Gastroenterology 2005 Only protein-protein interactions imex curation imex curation confocal experiment deleted mlh1_human DNA mismatch repair protein Mlh1 MutL protein homolog 1 MLH1 COCA2 protein protein human Homo sapiens Human MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC DIP protein P40692 original sequence version: 99 C9231FC406C2CA20 pms2_human Mismatch repair endonuclease PMS2 PMS1 protein homolog 2 DNA mismatch repair protein PMS2 PMS2 PMSL2 protein protein human Homo sapiens Human MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN B1B9547280ECAF9A mlh1-pms2-3 1 3 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell P40692:p.Pro28Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 15:53:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro28Leu' P40692:p.Ala29Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A S Tue Jun 30 19:22:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala29Ser' p.Thr45_Ile47delinsCysPhe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain TSI CF P40692:p.Gly67Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 19:18:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly67Arg' P40692:p.Glu71del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E . Tue Jun 30 17:47:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu71del' P40692:p.Cys77Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C R Tue Jun 30 19:09:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys77Arg' P40692:p.Phe80Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F V Tue Jun 30 18:49:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe80Val' P40692:p.Lys84Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 16:55:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys84Glu' P40692:p.Ile107Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I R Tue Jun 30 16:54:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile107Arg' P40692:p.Leu155Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 15:36:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu155Arg' P40692:p.Val213Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 19:53:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val213Met' P40692:p.Ile219Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I V Tue Jun 30 20:10:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile219Val' P40692:p.Ser247Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S P Tue Jun 30 17:33:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser247Pro' P40692:p.His329Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H P Tue Jun 30 19:41:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His329Pro' P40692:p.Ile330del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I . Tue Jun 30 18:42:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile330del' P40692:p.Lys443Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K Q Tue Jun 30 16:30:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys443Gln' P40692:p.Leu550Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 18:39:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu550Pro' P40692:p.Ala589Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A D Tue Jun 30 16:26:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala589Asp' P40692:p.Lys616del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K . Tue Jun 30 18:59:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys616del' P40692:p.Lys618Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:57:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys618Ala' P40692:p.Lys618Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K T Tue Jun 30 17:56:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys618Thr' p.Glu633_Glu663del required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain EGNLIGLPLLIDNYVPPLEGLPIFILRLATE . P40692:p.Tyr646Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 19:00:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr646Cys' P40692:p.Pro648Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 19:55:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro648Leu' P40692:p.Pro648Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P S Tue Jun 30 19:19:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro648Ser' P40692:p.Pro654Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 15:54:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro654Leu' P40692:p.Arg659Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R P Tue Jun 30 16:10:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg659Pro' P40692:p.Arg659Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 19:50:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg659Gln' P40692:p.Ala681Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 18:50:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala681Thr' P40692:p.Val716Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 17:32:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val716Met' P40692:p.Val612del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V . Tue Jun 30 17:13:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val612del' P40692:p.Ser93Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S G Tue Jun 30 20:09:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser93Gly' region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Table 2