UniProt UniProt http://www.uniprot.org Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy. Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy. Journal of cell science 2004 imex curation von Arnim CA., Tangredi MM., Peltan ID., Lee BM., Irizarry MC., Kinoshita A., Hyman BT. Alzheimers - Interactions investigated in the context of Alzheimers disease bhyman@partners.org 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-n2a Mouse neuroblastoma cells n2a Murine neuroblastoma cells confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry von Arnim CA., Tangredi MM., Peltan ID., Lee BM., Irizarry MC., Kinoshita A., Hyman BT. Alzheimers - Interactions investigated in the context of Alzheimers disease BACE mapped to BACE1 by mutant mapping. Experiment involving endogenous BACE could not be curated as this could not be mapped to a single protein bhyman@partners.org Accepted 2019-JUN-26 AT 15:59 BST AT 15:59 BST by LIVIAP Journal of cell science 2004 Only protein-protein interactions imex curation imex curation I've just added the mutant data as a comment as it more moves the interaction rather increases/decreases it. Haven't added a GO term as juxtanuclear does not seem to map to anything - I'm not even sure if they mean cytoplasmic or nuclear. Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy. Demonstration of BACE (beta-secretase) phosphorylation and its interaction with GGA1 in cells by fluorescence-lifetime imaging microscopy. Journal of cell science 2004 imex curation von Arnim CA., Tangredi MM., Peltan ID., Lee BM., Irizarry MC., Kinoshita A., Hyman BT. Alzheimers - Interactions investigated in the context of Alzheimers disease bhyman@partners.org 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-n2a Mouse neuroblastoma cells n2a Murine neuroblastoma cells fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret antibody detection identification by antibody antibody detection von Arnim CA., Tangredi MM., Peltan ID., Lee BM., Irizarry MC., Kinoshita A., Hyman BT. Alzheimers - Interactions investigated in the context of Alzheimers disease A FRET-variation, FLIM, for detecting intermolecular or intramolecular interactions that take place within a distance of 10nm or less BACE mapped to BACE1 by mutant mapping. Experiment involving endogenous BACE could not be curated as this could not be mapped to a single protein bhyman@partners.org Accepted 2019-JUN-26 AT 15:59 BST AT 15:59 BST by LIVIAP Journal of cell science 2004 Only protein-protein interactions imex curation imex curation I've just added the mutant data as a comment as it more moves the interaction rather increases/decreases it. Haven't added a GO term as juxtanuclear does not seem to map to anything - I'm not even sure if they mean cytoplasmic or nuclear. bace1_human Beta-secretase 1 BACE KIAA1149 Beta-site amyloid precursor protein cleaving enzyme 1 Membrane-associated aspartic protease 2 Memapsin-2 Aspartyl protease 2 BACE1 protein protein human Homo sapiens Human MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK DIP protein P56817 original sequence version: 161 377CE4C824ACEF05 377CE4C824ACEF05 gga1_human ADP-ribosylation factor-binding protein GGA1 Golgi-localized, gamma ear-containing, ARF-binding protein 1 Gamma-adaptin-related protein 1 GGA1 protein protein human Homo sapiens Human MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQANDNLTQVINLYKQLVRGEEVNGDATAGSIPGSTSALLDLSGLDLPPAGTTYPAMPTRPGEQASPEQPSASVSLLDDELMSLGLSDPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGLDDLDLLGKTLLQQSLPPESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQPVPTELSLASITVPLESIKPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFPPPETWGSL B0B6F089FA0F7DD5 bace1-gga1-3 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P56817:p.Ser498Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S D Tue Jun 30 20:24:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser498Asp' P56817:p.Ser498Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:24:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser498Ala' c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Fig. 2, 3 Table 1, 2 bace1-gga1-4 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 1 Mutation of BACE1 Ser-498 altered BACE1 localization by increasing (Ser498Asp) or decreasing (Ser498Ala) the concentration within perinuclear compartments