IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. The Journal of biological chemistry 2003 imex curation Comoletti D., Flynn R., Jennings LL., Chubykin A., Matsumura T., Hasegawa H., Südhof TC., Taylor P. pwtaylor@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Comoletti D., Flynn R., Jennings LL., Chubykin A., Matsumura T., Hasegawa H., Südhof TC., Taylor P. ref 9 (PMID: 9325340) reports: "cDNA encoding neurexin 1beta without an insert in splice site 4", which corresponds to Q63373-3. pwtaylor@ucsd.edu Accepted 2019-JUL-03 AT 08:47 BST AT 08:47 BST by ORCHARD The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation comment changed, however there must be a bug, cause I cannot view the annotations anymore... EDTA added as an inhibitor. Ca2+ is now a cofactor. Asn343Ala is now 'no effect'. Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. The Journal of biological chemistry 2003 imex curation Comoletti D., Flynn R., Jennings LL., Chubykin A., Matsumura T., Hasegawa H., Südhof TC., Taylor P. pwtaylor@ucsd.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Comoletti D., Flynn R., Jennings LL., Chubykin A., Matsumura T., Hasegawa H., Südhof TC., Taylor P. pwtaylor@ucsd.edu Accepted 2019-JUL-03 AT 08:47 BST AT 08:47 BST by ORCHARD The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation comment changed, however there must be a bug, cause I cannot view the annotations anymore... EDTA added as an inhibitor. Ca2+ is now a cofactor. Asn343Ala is now 'no effect'. nlgn1_rat Neuroligin-1 Nlgn1 Neuroligin I protein protein rat Rattus norvegicus (Rat) Rat MALPRCMWPNYVWRAMMACVVHRGSGAPLTLCLLGCLLQTFHVLSQKLDDVDPLVTTNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDVKRISKECARKPGKKICRKGDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVELVECLQKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTSTTTKVPSTDITLRPTRKNSTPVTSAFPTAKQDDPKQQPSPFSVDQRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTTNDLTHAPEEEIMSLQMKHTDLDHECESIHPHEVVLRTACPPDYTLAMRRSPDDVPLMTPNTITMIPNTIPGIQPLHTFNTFTGGQNNTLPHPHPHPHSHSTTRV mint Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses 90A18540245B789D calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q63373-3 Neurexin-1-beta Neurexin I-beta Beta-4B5A Nrxn1 protein protein rat Rattus norvegicus (Rat) Rat MYQRMLRCGAELGSPGGGSSGGAGGRLALLWIVPLTLSGLLGVAWGASSLGAHHIHHFHGSSKHHSVPIAIYRSPASLRGGHAGTTYIFSKGGGQITYKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEPSSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKEKQPSSAKSANKNKKNKDKEYYV 97B712B6C594042E nlgn1-1 2 3 unspecified role unspecified role neutral component neutral component purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 3 nrxn1-nlgn1-1 1 3 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Asn303 N-linked glycosylation nglycores N-glycosylated residue NGlycoRes mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain decreasing-ptm ptm decreasing an interaction decreasing-ptm Q62765:p.Asn343Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 16:34:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn343Ala' Q62765:p.Thr111Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 19:32:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr111Ala' Q62765:p.Ser549Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 20:03:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser549Ala' Q62765:p.Asn303Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 18:54:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn303Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 4 cofactor cofactor coenzyme unspecified role unspecified role 5 unspecified role unspecified role bait bait purified purified HIgG tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Table 1, Fig. 4, 5, 6, 7, 8, 9A and 10B CHEBI:64755 - EDTA(2−) To compare the relative NX1binding of the dif-ferent NL1 mutants, a range of NL1 concentrations extendingbetween 62.5 and 2000 nMwas injected and binding was mon-itored by SPR