IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Studies of the aggregation of mutant proteins in vitro provide insights into the genetics of amyloid diseases. Studies of the aggregation of mutant proteins in vitro provide insights into the genetics of amyloid diseases. Proceedings of the National Academy of Sciences of the United States of America 2002 imex curation Chiti F., Calamai M., Taddei N., Stefani M., Ramponi G., Dobson CM. cmd44@cam.ac.uk 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Chiti F., Calamai M., Taddei N., Stefani M., Ramponi G., Dobson CM. Origin species and expression system of Acp were extracted from ref. 45 (PMID:8679533). Mutation numbers were remapped to the Uniprot Sequence. Mutation Ser21Arg could not be annotated as no match was found in the Uniprot sequence. cmd44@cam.ac.uk Accepted 2019-OCT-16 AT 10:37 BST AT 10:37 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2002 Only protein-protein interactions imex curation imex curation acyp2_human Acylphosphatase-2 ACYP2 ACYP Acylphosphatase, muscle type isozyme Acylphosphate phosphohydrolase 2 protein protein human Homo sapiens Human MSTAQSLKSVDYEVFGRVQGVCFRMYTEDEARKIGVVGWVKNTSKGTVTGQVQGPEDKVNSMKSWLSKVGSPSSRIDRTNFSNEKTISKLEYSNFSIRY DIP protein P14621 original sequence version: 93 AEDE40E45B6207A0 acp_fibrils-1 1 2 unspecified role unspecified role neutral component neutral component purified purified P14621:p.Glu30Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 17:58:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Lys' P14621:p.Glu30Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E Q Tue Jun 30 18:05:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Gln' P14621:p.Glu30Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E R Tue Jun 30 17:48:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Arg' P14621:p.Ser9His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S H Tue Jun 30 16:12:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser9His' P14621:p.Glu56Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E Q Tue Jun 30 18:46:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu56Gln' P14621:p.Arg98Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 19:17:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gln' P14621:p.Arg24Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 18:54:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg24Gln' P14621:p.Ser44Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 19:06:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser44Glu' P14621:p.Arg78Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 17:28:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Glu' P14621:p.Lys89Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K N Tue Jun 30 19:32:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys89Asn' P14621:p.Lys89Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K Q Tue Jun 30 19:11:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys89Gln' P14621:p.Glu91His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E H Tue Jun 30 15:38:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu91His' P14621:p.Ser93His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S H Tue Jun 30 19:59:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser93His' P14621:p.Arg98Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 17:37:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Glu' ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role neutral component neutral component purified purified P14621:p.Glu30Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 19:39:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Lys' P14621:p.Glu30Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E Q Tue Jun 30 17:31:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Gln' P14621:p.Glu30Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E R Tue Jun 30 19:07:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu30Arg' P14621:p.Ser9His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S H Tue Jun 30 19:48:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser9His' P14621:p.Glu56Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E Q Tue Jun 30 15:54:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu56Gln' P14621:p.Arg98Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 16:32:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gln' P14621:p.Arg24Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 18:12:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg24Gln' P14621:p.Ser44Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S E Tue Jun 30 19:37:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser44Glu' P14621:p.Arg78Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 19:58:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Glu' P14621:p.Lys89Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K N Tue Jun 30 16:31:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys89Asn' P14621:p.Lys89Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K Q Tue Jun 30 18:28:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys89Gln' P14621:p.Glu91His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E H Tue Jun 30 19:04:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu91His' P14621:p.Ser93His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S H Tue Jun 30 16:00:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser93His' P14621:p.Arg98Glu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 18:59:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, Fig. 3A Fig.3a describes the increase of ThT fluorescence resulting from aggregation of wild-type AcP and some representative mutants under these conditions.