UniProt UniProt http://www.uniprot.org A novel structural model for regulation of clathrin function. A novel structural model for regulation of clathrin function. The EMBO journal 1997 imex curation Pishvaee B., Munn A., Payne GS. 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Pishvaee B., Munn A., Payne GS. Cells were subject to several rounds of detergent lysis and centrifugation to create and purify spheroplasts. The spheroplasts were lysed by glass beads, and then centrifuged. The supernatant was applied to a Sepharose CL-4B column. Accepted 2018-FEB-01 AT 10:20 GMT AT 10:20 GMT by LIVIAP The EMBO journal 1997 imex curation A novel structural model for regulation of clathrin function. A novel structural model for regulation of clathrin function. The EMBO journal 1997 imex curation Pishvaee B., Munn A., Payne GS. 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pishvaee B., Munn A., Payne GS. Accepted 2018-FEB-01 AT 10:20 GMT AT 10:20 GMT by LIVIAP The EMBO journal 1997 imex curation clh_yeast Clathrin heavy chain CHC1 YGL206C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLPIEFTELVDLMSLGISPQFLDFRSTTFESDHFVTVRETKDGTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSFTLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFVANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQVFVTGNRNATTGAGELRIIEIDHDASLPSQYQKETTDIFFPPDATNDFPIAVQVSEKYGIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVEISTSQIVPYILNKLSNVALALIVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAASSTSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPYCQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQQGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSEKAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEAAAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLYKSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEKRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHYCEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIHRRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVALQGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKALKVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENVIDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANLDHVGDKLFENKEYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLVNDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHMGMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWDNAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRLDIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYDKFDQLGLASRLESHKLIFFKKIGALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQDPKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKKEQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF 622F3083DF2FE315 622F3083DF2FE315 clc1_yeast Clathrin light chain CLC1 YGR167W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEKFPPLEDQNIDFTPNDKKDDDTDFLKREAEILGDEFKTEQDDILETEASPAKDDDEIRDFEEQFPDINSANGAVSSDQNGSATVSSGNDNGEADDDFSTFEGANQSTESVKEDRSEVVDQWKQRRAVEIHEKDLKDEELKKELQDEAIKHIDDFYDSYNKKKEQQLEDAAKEAEAFLKKRDEFFGQDNTTWDRALQLINQDDADIIGGRDRSKLKEILLRLKGNAKAPGA 27BAB175780EC8B3 chc1-clc1-4 2 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P22137:p.[Ser1331Phe;Ser1347Phe;Pro1553Leu;Glu1590Lys] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain E K Wed May 02 20:08:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,pro1553leu,glu1590lys' Tue Jun 30 16:58:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Pro1553Leu;Glu1590Lys]' P22137:p.Glu1590Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:47:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1590lys' Tue Jun 30 19:39:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1590Lys' P22137:p.[Ser1347Phe;Pro1553Leu;Glu1590Lys] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:57:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1347phe,pro1553leu,glu1590lys' Tue Jun 30 18:56:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1347Phe;Pro1553Leu;Glu1590Lys]' P22137:p.Glu1590Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:29:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1590ala' Tue Jun 30 17:52:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1590Ala' P22137:p.[Ser1331Phe;Ser1347Phe;Glu1590Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F Wed May 02 16:19:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,glu1590ala' Tue Jun 30 19:01:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Glu1590Ala]' P22137:p.[Ser1331Phe;Ser1347Phe;Pro1553Leu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F Wed May 02 20:27:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,pro1553leu' Tue Jun 30 19:32:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Pro1553Leu]' P22137:p.[Ser1331Phe;Ser1347Phe] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F Wed May 02 16:33:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe' Tue Jun 30 16:53:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe]' P22137:p.[Ser1347Phe;Pro1553Leu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain P L Wed May 02 20:16:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1347phe,pro1553leu' Tue Jun 30 17:38:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1347Phe;Pro1553Leu]' P22137:p.[Ser1331Phe;Ser1347Phe;Glu1590Lys] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:08:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,glu1590lys' Tue Jun 30 15:59:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Glu1590Lys]' P22137:p.[Pro1553Leu;Glu1590Lys] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:29:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro1553leu,glu1590lys' Tue Jun 30 18:50:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro1553Leu;Glu1590Lys]' physical association physical association Fig. 2A, Fig. 3A-D, Fig 6 Cell extracts were prepared under non-denaturing conditions, Clc was immunoprecipitated with affinity-purified polyclonal anti-bodies, and the precipitates were analyzed by SDS–PAGE and immunoblotting for both Clc and Chc (Figure 2A). clc1-chc1-3 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P22137:p.Glu1590Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:49:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1590ala' Tue Jun 30 16:02:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1590Ala' P22137:p.[Ser1331Phe;Ser1347Phe;Glu1590Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:39:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,glu1590ala' Tue Jun 30 18:31:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Glu1590Ala]' P22137:p.[Ser1331Phe;Ser1347Phe;Pro1553Leu;Glu1590Lys] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:08:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,pro1553leu,glu1590lys' Tue Jun 30 18:05:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Pro1553Leu;Glu1590Lys]' P22137:p.Glu1590Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:44:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1590lys' Tue Jun 30 19:58:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu1590Lys' P22137:p.[Ser1331Phe;Ser1347Phe;Pro1553Leu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F Wed May 02 14:41:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe,pro1553leu' Tue Jun 30 18:28:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe;Pro1553Leu]' P22137:p.[Ser1331Phe;Ser1347Phe] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S F certain certain sequence position certain certain certain sequence position certain S F Wed May 02 19:03:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1331phe,ser1347phe' Tue Jun 30 17:12:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1331Phe;Ser1347Phe]' physical association physical association Fig. 2B, 3E, 3F, 6 Cell extracts were prepared under non-denaturing conditions, Clc was immunoprecipitated with affinity-purified polyclonal anti-bodies, and the precipitates were analyzed by SDS–PAGE and immunoblotting for both Clc and Chc (Figure 2A).