UniProt UniProt http://www.uniprot.org CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. Frontiers in Immunology 2017 imex curation Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es Accepted 2019-SEP-26 AT 13:47 BST AT 13:47 BST by MARTA Frontiers in Immunology 2017 imex curation CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. Frontiers in Immunology 2017 imex curation Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es Accepted 2019-SEP-26 AT 13:47 BST AT 13:47 BST by MARTA Frontiers in Immunology 2017 imex curation CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. CD9 Controls Integrin α5β1-Mediated Cell Adhesion by Modulating Its Association With the Metalloproteinase ADAM17. Frontiers in Immunology 2017 imex curation Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es 03-Oct-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Machado-Pineda Y., Cardeñes B., Reyes R., López-Martín S., Toribio V., Sánchez-Organero P., Suarez H., Grötzinger J., Lorenzen I., Yáñez-Mó M., Cabañas C. ccabanas@cbm.csic.es Accepted 2019-SEP-26 AT 13:47 BST AT 13:47 BST by MARTA Frontiers in Immunology 2017 imex curation ada17_human Disintegrin and metalloproteinase domain-containing protein 17 ADAM17 CSVP TACE TNF-alpha-converting enzyme TNF-alpha convertase Snake venom-like protease protein protein human Homo sapiens Human MRQSLLFLTSVVPFVLAPRPPDDPGFGPHQRLEKLDSLLSDYDILSLSNIQQHSVRKRDLQTSTHVETLLTFSALKRHFKLYLTSSTERFSQNFKVVVVDGKNESEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVHRVKRRADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIEQIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYSPVGKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGNAEDDTVCLDLGKCKDGKCIPFCEREQQLESCACNETDNSCKVCCRDLSGRCVPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDKQYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDSHMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC DIP protein P78536 original sequence version: 170 DIP protein P78536 original sequence version: 184 5B1032F6B88A837F cd9_human CD9 antigen CD9 MIC3 TSPAN29 5H9 antigen Cell growth-inhibiting gene 2 protein Leukocyte antigen MIC3 Motility-related protein Tetraspanin-29 p24 GIG2 protein protein human Homo sapiens Human MPVKGGTKCIKYLLFGFNFIFWLAGIAVLAIGLWLRFDSQTKSIFEQETNNNNSSFYTGVYILIGAGALMMLVGFLGCCGAVQESQCMLGLFFGFLLVIFAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV F68333E0C20611D8 itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C itav_human Integrin alpha-V ITGAV VNRA Vitronectin receptor subunit alpha MSK8 Vitronectin receptor VTNR protein protein human Homo sapiens Human MAFPPRRRLRLGPRGLPLLLSGLLLPLCRAFNLDVDSPAEYSGPEGSYFGFAVDFFVPSASSRMFLLVGAPKANTTQPGIVEGGQVLKCDWSSTRRCQPIEFDATGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAPLYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSQDIDADGQGFCQGGFSIDFTKADRVLLGGPGSFYWQGQLISDQVAEIVSKYDPNVYSIKYNNQLATRTAQAIFDDSYLGYSVAVGDFNGDGIDDFVSGVPRAARTLGMVYIYDGKNMSSLYNFTGEQMAAYFGFSVAATDINGDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQRASGDFQTTKLNGFEVFARFGSAIAPLGDLDQDGFNDIAIAAPYGGEDKKGIVYIFNGRSTGLNAVPSQILEGQWAARSMPPSFGYSMKGATDIDKNGYPDLIVGAFGVDRAILYRARPVITVNAGLEVYPSILNQDNKTCSLPGTALKVSCFNVRFCLKADGKGVLPRKLNFQVELLLDKLKQKGAIRRALFLYSRSPSHSKNMTISRGGLMQCEELIAYLRDESEFRDKLTPITIFMEYRLDYRTAADTTGLQPILNQFTPANISRQAHILLDCGEDNVCKPKLEVSVDSDQKKIYIGDDNPLTLIVKAQNQGEGAYEAELIVSIPLQADFIGVVRNNEALARLSCAFKTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSEMDTSVKFDLQIQSSNLFDKVSPVVSHKVDLAVLAAVEIRGVSSPDHVFLPIPNWEHKENPETEEDVGPVVQHIYELRNNGPSSFSKAMLHLQWPYKYNNNTLLYILHYDIDGPMNCTSDMEINPLRIKISSLQTTEKNDTVAGQGERDHLITKRDLALSEGDIHTLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLLWTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTWGIQPAPMPVPVWVIILAVLAGLLLLAVLVFVMYRMGFFKRVRPPQEEQEREQLQPHENGEGNSET DIP protein P06756 original sequence version: 136 DIP protein P06756 original sequence version: 145 DIP protein P06756 original sequence version: 174 DIP protein P06756-1 original sequence version: 182 DIP protein P06756 original sequence version: 149 364EE25C5303A2D7 adam17-itgav-1 2 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction CD9 (P21926) Fig. 6 To explore the organization α5β1 integrin and ADAM17 at the plasma membrane closer to the molecular level, we chose to use in situ Proximity Ligation Assays (PLA), that have been previously employed to demonstrate the cis interaction between ADAM17 and integrin α5β1 (6). itgb1-adam17-1 3 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 6 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 physical association physical association CD9 (P21926) Fig. 7a To biochemically confirm these data, we immunoprecipitated (IP) α5β1 integrin from Colo320 and Colo320-CD9 cells using mAb Lia1/2, which recognizes an extracellular epitope on the β1 subunit of the integrin, and analyzed by immunobloting the amount of co-immunoprecipitated ADAM17. cd9-adam17-1 3 5 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-colo_320 Human colon adenocarcinoma colo_320 Human colorectal adenocarcinoma Colo320 association association CD9 (P21926) Fig. 7b adam17-itgav-2 1 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization CD9 (P21926) Fig. 5C