DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An electron transfer path connects subunits of a mycobacterial respiratory supercomplex. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex. Science (New York, N.Y.) 2018 imex curation Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z., Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column fingerprinting peptide massfingerprinting fingerprinting Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn Accepted 2020-MAR-16 AT 08:39 GMT AT 08:39 GMT by MGT Science (New York, N.Y.) 2018 Only protein-protein interactions imex curation imex curation An electron transfer path connects subunits of a mycobacterial respiratory supercomplex. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex. Science (New York, N.Y.) 2018 imex curation Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z., Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn Accepted 2020-MAR-16 AT 08:39 GMT AT 08:39 GMT by MGT Science (New York, N.Y.) 2018 Only protein-protein interactions imex curation imex curation a0r562_mycs2 MSMEG_6078 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MNRFSSRAGLAVCGLATAVALTACSAGQISQTTTQEPAVNGVNAQAGQVSLRNVHLRAPQQTDYVEPGTTVELLFVAANDSTEGSNKLKSITSDVGEVTLTGDSTVPADGVLIVGEPDGQIQAVENAEAADAVTAEVELTKPITNGLLYDFTFTFEDGETTVAVPISAGEQPRRPVPPAGPGSSEH 93BDF99312FAAB20 a0a8b4r8z7_mycsm ctaD_2 NCTC7017_06542 protein protein mycsm Mycobacterium smegmatis MGPKGNLIYKLITTTDHKLIGIMYCVVCFAFFLVGGLMALFMRTELAMPGLQFLSNEQFNQLFTMHGTVMLLFYATPIVFGFANLVLPLQIGAPDVAFPRLNALSFWLFLFGALIAIAGFITPGGAADFGWTAYSPLTDAIHSPGAGGDLWIMGLAVGGLGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSILVLIAFPILTAALFGLAADRHLGAHIYDPANGGVLLWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPIFGYTTLIYATLAIAALSVAVWAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLITFLLGGLSGVLLASPPLDFHVTDSYFVIAHFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGDEGMPRRYADYLPTDGFTTLNVISTVGAFILGVSMLPFVWNVFKSWRYGEPVTVDDPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERMRAEAHVGRAHHPELETADKSS 1AD131C9DA3C9B19 a0qpw0_mycs2 MSMEG_0537 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MAAERKKDRPNLLQYIAYSYGRRLPDSMREWVAHDLADHGAVRRHMIRMAIPPALVLAPFWLLPASLYVHIEMTVPIYIWSLLMALALNKVWRRHRLAQHGLDPNLVDEIRYKKQAHIHEDYIRRFGPRPESAKFQSNSSPF 2038F127D03F73F8 a0r1b6_mycs2 MSMEG_4693 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSTALTHGLIGGVPLVLFAVLALIFLTRKGPHPDTYKMSDPWTHAPILWAAEEPREHGHGGHGHDSHGVVIGGGASGKW 80260C63A1C54700 y4692_mycs2 Uncharacterized protein MSMEG_4692/MSMEI_4575 MSMEG_4692 MSMEI_4575 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MASGDIATVANAELDLPYGSALTSSGRISAVTEPGELSVHYPFPTMDLVVLDDALKYGSRAAKARFAVYIGPLGADTAATAREILANVPTPENAVLLAVSPDQRAIEVVYGADVKGRGIESAAPLGVSAAAASFKEGNLIDGLISAVRVMSAGVSPA 0681183BDB1ACB66 i7fph7_mycs2 Cytochrome c oxidase polypeptide 4 Cytochrome aa3 subunit 4 Cytochrome c oxidase polypeptide IV MSMEI_4166 ctaF protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MHIEARLFEILTAFFALAAVVYAVLTAMFATGGVEWAGTTALVLTTGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFFAPHSWWPILISLSFSTAAVGAALWLPWLIAAGVAFVITSVCGLVFEYYWGPEKH EA46462299D8B7CF i7gd61_mycs2 Cytochrome bc1 complex Rieske iron-sulfur subunit qcrA Cytochrome bc1 reductase complex subunit QcrA MSMEI_4162 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MDYRNGGRHRCGHVDRIASMSQDSPDIKGTDAPGQTGVPGQPTDAELAEMSREELVKLGGKIDGVETIFKEPRWPVPGTKAEKRTERLVAYWLMLGGLSGLALLLVFLFWPWEYQPFGSEGEFLYSLATPLYGLTFGLSILSIGIGAVLFQKKFIPEEISVQDRHDGRSPEVHRKTVAANLTDALEGSTLKRRKVIGLSLGIGLGAFGAGTLVAFIGGLIKNPWKPVVPTAEGKKAVLWTSGWTPRFKGETIYLARATGRPGESPFVKMRPEDIDAGGMETVFPWRESDGDGTTVESEHKLTEIAMGVRNPVMLIRIKPADMHRVIKRKGQESFNFGELFAYTKVCSHLGCPSSLYEQQTYRILCPCHQSQFDALEFAKPIFGPAARALAQLPITIDEDGYLVANGDFVEPVGPAFWERKS 16D7CA1D17AE7E4B a0qvh4_mycs2 MSMEG_2575 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MANTEVERHTGVDVEDVPSAEWGWSHMPIGVMHIGGLLSAAFLLVMMRGNHVGHVEDWFLIGFAAVIVALVGRNWWLRRRGWIR 764190BEF2EC721B i7gd63_mycs2 Cytochrome aa3 subunit 2 ctaC MSMEI_4167 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTPRGFRVVALSIVLGGSALLLSGCSWSDALALGWPTGITPEAKLNRELWIGSVIASFAVGAIVWGLIFWTSAFHRKKATDTELPRQFGYNMPLELTLTVIPFLIISVLFYFTVVVQERMMHKDPNPEVVIDVTAFQWNWKFGYQKIAFADGSFDYDGADPERKEAMTSRPEGKDEHGIEKVGPIRGMTPEDRTYLNFDKIETLGTSSEIPVLVLPAGKRIEFVLNSADVIHGFWVPEFLFKRDVLPEPKANNSDNVFQVSEIQQTGAFVGRCTEMCGTFHAMMNFEVRVVEPNDFKAYIDQRNAGKTNAEALAAINQPPLAITTEPFESRRGELVPQASK 7266657D9FC21247 i7g4q8_mycs2 Cytochrome aa3 subunit 3 ctaE Probable cytochrome c oxidase subunit 3 MSMEI_4160 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLFAMYFTARAQAGGAWPPEPTELNLALAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRWYVITFLMGLFFVLGQGYEYIHLVEHGTTIPGSAYGSVFYLATGFHGLHVIGGLVAFVLLLARTKMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR 6330A4645BDAEBDC ctad cytochrome c oxidase subunit I protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MVAEAPPIGELEARRPFPERMGPKGNLIYKLITTTDHKLIGIMYCVVCFAFFLVGGLMALFMRTELAMPGLQFLSNEQFNQLFTMHGTVMLLFYATPIVFGFANLVLPLQIGAPDVAFPRLNALSFWLFLFGALIAIAGFITPGGAADFGWTAYSPLTDAIHSPGAGGDLWIMGLAVGGLGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSILVLIAFPILTAALFGLAADRHLGAHIYDPANGGVLLWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPIFGYTTLIYATLAIAALSVAVWAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLITFLLGGLSGVLLASPPLDFHVTDSYFVIAHFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGDEGMPRRYADYLPTDGFTTLNVISTVGAFILGVSMLPFVWNVFKSWRYGEPVTVDDPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERMRAEAHVGRAHHPELETADKSS a0r049_mycs2 Cytochrome aa3 subunit 3 Probable cytochrome c oxidase subunit 3 MSMEG_4260 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLFAMYFTARAQAGGAWPPEPTELNLALAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRWYVITFLMGLFFVLGQGYEYIHLVEHGTTIPGSAYGSVFYLATGFHGLHVIGGLVAFVLLLARTKMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR 6330A4645BDAEBDC a0qr51_mycs2 MSMEG_0987 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MRHRLERTAVPYVSYVLVLVGFVAFGALVASLAYGSHIPAGIAGAVLAGSLTGAVIGFRAAARRLRADRGDAHNVSIFDTPLEPEMVDRYARRYRATGTDAEESAVAVLPAAETAVARERRAA 38025E8F69443FF8 a0a8b4r3r6_mycsm qcrC NCTC7017_04661 protein protein mycsm Mycobacterium smegmatis MTSKSRRRLRRRLSAGLLLLIGLAVAGGVAATLTPQPQVAVADESQSALLRTGKQLFETSCVSCHGANLQGVPDRGPSLIGTGEAAVYFQVSTGRMPAMRGEAQAPSKPPHFDESQIDALGAYVQANGGGPTVPRDDHGAVAQESLIGGDVARGGDLFRLNCASCHNFTGKGGALSSGKYAPDLGDANPAQIYTAMLTGPQNMPKFSDRQLTPDEKRDIVAYVRESAETPSYGGYGLGGFGPAPEGMAMWIIGMVAAIGVAMWIGSRA E485E2C3D0A7EBA6 a0r057_mycs2 Cytochrome aa3 subunit 2 MSMEG_4268 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTPRGFRVVALSIVLGGSALLLSGCSWSDALALGWPTGITPEAKLNRELWIGSVIASFAVGAIVWGLIFWTSAFHRKKATDTELPRQFGYNMPLELTLTVIPFLIISVLFYFTVVVQERMMHKDPNPEVVIDVTAFQWNWKFGYQKIAFADGSFDYDGADPERKEAMTSRPEGKDEHGIEKVGPIRGMTPEDRTYLNFDKIETLGTSSEIPVLVLPAGKRIEFVLNSADVIHGFWVPEFLFKRDVLPEPKANNSDNVFQVSEIQQTGAFVGRCTEMCGTFHAMMNFEVRVVEPNDFKAYIDQRNAGKTNAEALAAINQPPLAITTEPFESRRGELVPQASK 7266657D9FC21247 a0qvz6_mycs2 MSMEG_2753 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MKQSHELSFDDDFDAEARPVLITRAAPAYEVQHRQRVRKYLTLMAFRIPALILAAVAYNIWENGLISLAIIAVSIPLPWMAVLIANDRPPRKREEPRRYAGRGEKIPLFPTAERPALMSDLHREPEAPPQPGTADFRGHPAS 51EAB6B89A2AF9D3 a0qqq1_mycs2 Superoxide dismutase [Cu-Zn] sodC MSMEG_0835 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MLKPVSVAVLFATPVLALSACSPPGETASSEPGTTPAIWTGSPSPAAPSGEDHGGGHGAGAAGAGETLTAELKTADGTSVATADFQFADGFATVTIETTTPGRLTPGFHGVHIHSVGKCEANSVAPTGGAPGDFNSAGGHFQVSGHSGHPASGDLSSLQVRADGSGKLVTTTDAFTAEDLLDGAKTAIIIHEKADNFANIPPERYQQVNGAPGPDQTTMATGDAGSRVACGVISAG 7D82A275F4F1217B a0r052_mycs2 Cytochrome bc1 complex cytochrome b subunit Cytochrome bc1 reductase complex subunit QcrB MSMEG_4263 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSPDFAKLAAAQGDAIDSRYHPSAAVRRQLNKVFPTHWSFLLGEIALYSFIILLLTGVWLTLFFDPSMAHVTYDGVYQPLRGVQMSRAYETALDISFEVRGGLFVRQVHHWAALMFAASIMVHLARIFFTGAFRRPREANWVIGSLLLILAMFEGFFGYSLPDDLLSGTGIRAALSGITMGIPVIGTWMHWALFGGDFPGEILIPRLYALHILLIPGIILALIGAHLALVWFQKHTQFPGPGRTETNVVGVRVMPVFAVKSGAFFAMITGVLGLMGGLLTINPIWNLGPYKPSQVSAGSQPDFYMMWTDGLIRLWPAWEFYPFGHTIPQGVWVAVGMGLVFALLIAYPFIEKKVTGDDAHHNLLQRPRDVPVRTAIGSMAIALYLLLTFACMNDIIALKFHISLNATTWIGRIGMVVLPAIVYFVAYRWAISLQRSDREVLEHGVETGIIKRLPHGAYVELHQPLGPVDEHGHPIPLEYAGAPLPKRMNKLGSGGAPGTGSFLFPDPAVEHEALTEAAHASEHKSLTALKEHQDRIHGNGETNGHH 1BCED659CE3520AA i7fgs8_mycs2 Cytochrome bc1 complex cytochrome b subunit qcrB Cytochrome bc1 reductase complex subunit QcrB MSMEI_4163 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSPDFAKLAAAQGDAIDSRYHPSAAVRRQLNKVFPTHWSFLLGEIALYSFIILLLTGVWLTLFFDPSMAHVTYDGVYQPLRGVQMSRAYETALDISFEVRGGLFVRQVHHWAALMFAASIMVHLARIFFTGAFRRPREANWVIGSLLLILAMFEGFFGYSLPDDLLSGTGIRAALSGITMGIPVIGTWMHWALFGGDFPGEILIPRLYALHILLIPGIILALIGAHLALVWFQKHTQFPGPGRTETNVVGVRVMPVFAVKSGAFFAMITGVLGLMGGLLTINPIWNLGPYKPSQVSAGSQPDFYMMWTDGLIRLWPAWEFYPFGHTIPQGVWVAVGMGLVFALLIAYPFIEKKVTGDDAHHNLLQRPRDVPVRTAIGSMAIALYLLLTFACMNDIIALKFHISLNATTWIGRIGMVVLPAIVYFVAYRWAISLQRSDREVLEHGVETGIIKRLPHGAYVELHQPLGPVDEHGHPIPLEYAGAPLPKRMNKLGSGGAPGTGSFLFPDPAVEHEALTEAAHASEHKSLTALKEHQDRIHGNGETNGHH 1BCED659CE3520AA a0r056_mycs2 Cytochrome c oxidase polypeptide 4 Cytochrome aa3 subunit 4 Cytochrome c oxidase polypeptide IV MSMEG_4267 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MHIEARLFEILTAFFALAAVVYAVLTAMFATGGVEWAGTTALVLTTGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFFAPHSWWPILISLSFSTAAVGAALWLPWLIAAGVAFVITSVCGLVFEYYWGPEKH EA46462299D8B7CF i7fph1_mycs2 Cytochrome bc1 complex cytochrome c subunit qcrC MSMEI_4161 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MDRAVRHHLLRPMNIGSPMSSMRRGSMTSKSRRRLRRRLSAGLLLLIGLAVAGGVAATLTPQPQVAVADESQSALLRTGKQLFETSCVSCHGANLQGVPDRGPSLIGTGEAAVYFQVSTGRMPAMRGEAQAPSKPPHFDESQIDALGAYVQANGGGPTVPRDDHGAVAQESLIGGDVARGGDLFRLNCASCHNFTGKGGALSSGKYAPDLGDANPAQIYTAMLTGPQNMPKFSDRQLTPDEKRDIVAYVRESAETPSYGGYGLGGFGPAPEGMAMWIIGMVAAIGVAMWIGSRA EB14066D02A79524 a0r562_mycs2-ctac-1 1 15 unspecified role unspecified role neutral component neutral component purified purified 5 unspecified role unspecified role neutral component neutral component purified purified 18 unspecified role unspecified role neutral component neutral component purified purified 6 unspecified role unspecified role neutral component neutral component purified purified 3 unspecified role unspecified role neutral component neutral component purified purified 19 unspecified role unspecified role neutral component neutral component purified purified 7 unspecified role unspecified role neutral component neutral component purified purified 8 unspecified role unspecified role neutral component neutral component purified purified 12 unspecified role unspecified role neutral component neutral component purified purified 11 unspecified role unspecified role neutral component neutral component purified purified 16 unspecified role unspecified role neutral component neutral component purified purified 21 unspecified role unspecified role neutral component neutral component purified purified 9 unspecified role unspecified role neutral component neutral component purified purified 10 unspecified role unspecified role neutral component neutral component purified purified 13 unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig S1D, Table S1 a0r049_mycs2-a0r052_mycs2-1 2 10 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 19 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 3 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 7 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 6 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 22 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 14 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 4 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 17 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 23 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 20 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig 1B, Table S2, Fig S3A-G, movie S1