DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Nature 2018 imex curation Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu Accepted 2018-NOV-06 AT 09:50 GMT AT 09:50 GMT by ORCHARD Nature 2018 imex curation Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Nature 2018 imex curation Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu Accepted 2018-NOV-06 AT 09:50 GMT AT 09:50 GMT by ORCHARD Nature 2018 imex curation Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Crystal structure of the natural anion-conducting channelrhodopsin GtACR1. Nature 2018 imex curation Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Kim YS., Kato HE., Yamashita K., Ito S., Inoue K., Ramakrishnan C., Fenno LE., Evans KE., Paggi JM., Dror RO., Kandori H., Kobilka BK., Deisseroth K. deissero@stanford.edu Accepted 2018-NOV-06 AT 09:50 GMT AT 09:50 GMT by ORCHARD Nature 2018 imex curation l1j207_guitc GUITHDRAFT_111593 protein protein Guillardia theta CCMP2712 Guillardia theta CCMP2712 MSSITCDPAIYGEWSRENQFCVEKSLITLDGIKYVQLVMAVVSACQVFFMVTRAPKVPWEAIYLPTTEMITYSLAFTGNGYIRVANGKYLPWARMASWLCTCPIMLGLVSNMALVKYKSIPLNPMMIAASSICTVFGITASVVLDPLHVWLYCFISSIFFIFEMVVAFAIFAITIHDFQTIGSPMSLKVVERLKLMRIVFYVSWMAYPILWSFSSTGACIMSENTSSVLYLLGDALCKNTYGILLWATTWGLLNGKWDRDYVKGRNVDGTLMPEYEQDLEKGNTERYEDARAGETFEVKMFGRTLTSVRRSRRRPRRDIVFNGGDRNRLSDSDSERRRRRKKYQRKDSESENSSDESMGSEHEKNRRKGKKKNKENRRSAPPKEDSTSDTLRDAEIKRIMDLMKRNAGSDIPGLDGDGRLRDDGGLIPTRNEEHDDSA 473CE168CF7D19F5 l1j207_guith-1 1 4 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection disulfide bond disulfide bond disulfide bridge SS-bond Fig 1a, Extended Data Fig 1, Extended Data Fig 2d l1j207_guith-2 2 4 unspecified role unspecified role neutral component neutral component purified purified L1J207:p.Cys21Ser C21S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C S Wed Sep 26 13:19:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys212Ser' Tue Jun 30 17:33:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys21Ser' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FLAG tag FLAG tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range eGFP tag eGFP tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range L1J207:p.Cys219Ser C219S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C S Tue Jun 30 18:53:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys219Ser' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Extended Data Fig 5d l1j207_guith-3 3 4 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain required to bind required to bind required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection disulfide bond disulfide bond disulfide bridge SS-bond Extended Data Fig 5e