DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization. Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization. Nature 2018 imex curation Vještica A., Merlini L., Nkosi PJ., Martin SG. Sophie.Martin@unil.ch 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vještica A., Merlini L., Nkosi PJ., Martin SG. Sophie.Martin@unil.ch Accepted 2019-FEB-07 AT 09:06 GMT AT 09:06 GMT by ORCHARD Nature 2018 imex curation Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization. Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization. Nature 2018 imex curation Vještica A., Merlini L., Nkosi PJ., Martin SG. Sophie.Martin@unil.ch 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) pull down pull down anti tag western anti tag western blot anti tag western Vještica A., Merlini L., Nkosi PJ., Martin SG. Sophie.Martin@unil.ch Accepted 2019-FEB-07 AT 09:06 GMT AT 09:06 GMT by ORCHARD Nature 2018 imex curation matpi_schpm Mating-type P-specific polypeptide Pi mat2-Pi matPi SPMTR.02 protein protein schpm Schizosaccharomyces pombe (fission yeast) Fission yeast MKRVAVLLKTVMCEFLKCDYNGYDRIISLLRRILTLICTPNLNGLTIKRVIDSMQSLEYIKQTCNFKLQMCISSMAFKRNNALQNCNHYAWCDDHCSDIGRPMTTVRGQCSKCTKPHLMRWLLLHYDNPYPSNSEFYDLSAATGLTRTQLRNWFSNRRR 226371B0446AE8EA matmi_schpo Mating-type M-specific polypeptide Mi mat1-Mi mat1-Mm matMi SPBC23G7.17c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSAEDLFTIQILCDQIELKLASIVINSNIKLQLKRKKKTQQL AAF0575237606622 mat1_mi-mat2_pi-1 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed GFP tag GFP tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast 4 unspecified role unspecified role bait bait Halo Tag Halo Tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast physical association physical association Fig. 2d mat1_mi-mat2_pi-2 2 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed mbp tag mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 2e