DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the recognition of Sonic Hedgehog by human Patched1. Structural basis for the recognition of Sonic Hedgehog by human Patched1. Science (New York, N.Y.) 2018 imex curation Gong X., Qian H., Cao P., Zhao X., Zhou Q., Lei J., Yan N. nyan@princeton.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gong X., Qian H., Cao P., Zhao X., Zhou Q., Lei J., Yan N. nyan@princeton.edu Accepted 2018-OCT-15 AT 15:03 BST AT 15:03 BST by ORCHARD Science (New York, N.Y.) 2018 imex curation Structural basis for the recognition of Sonic Hedgehog by human Patched1. Structural basis for the recognition of Sonic Hedgehog by human Patched1. Science (New York, N.Y.) 2018 imex curation Gong X., Qian H., Cao P., Zhao X., Zhou Q., Lei J., Yan N. nyan@princeton.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Gong X., Qian H., Cao P., Zhao X., Zhou Q., Lei J., Yan N. nyan@princeton.edu Accepted 2018-OCT-15 AT 15:03 BST AT 15:03 BST by ORCHARD Science (New York, N.Y.) 2018 imex curation shh_human Sonic hedgehog protein Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains SHH HHG-1 protein protein human Homo sapiens Human MLLLARCLLLVLVSSLLVCSGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALGPRALFASRVRPGQRVYVVAERDGDRRLLPAAVHSVTLSEEAAGAYAPLTAQGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDRGGDSGGGDRGGGGGRVALTAPGAADAPGAGATAGIHWYSQLLYQIGTWLLDSEALHPLGMAVKSS DIP protein Q15465 original sequence version: 187 DD687AFA582A4749 q13635-1 Protein patched homolog 1 PTCH1 PTCH 1B protein protein human Homo sapiens Human MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN F2937247BC812F85 shh-ptch1-2 1 3 unspecified role unspecified role bait bait purified purified Q15465:p.Arg153Glu R153E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 18:39:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg153Glu' MBP tag MBP tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q15465:p.[Tyr44Ala;Lys45Ala] Y44A/K45A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:48:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr44Ala;Lys45Ala]' Q15465:p.Arg155Glu R155E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 19:17:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg155Glu' Q15465:p.[Ser177Ala;Lys178Ala] S177A/K178A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain K A Tue Sep 04 15:07:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser177Ala,Lys178Ala]' Tue Jun 30 18:29:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser177Ala;Lys178Ala]' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig. 2C ptch1-shh-5 2 4 unspecified role unspecified role neutral component neutral component purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1, Table 1 shh-ptch1-1 1 3 unspecified role unspecified role bait bait purified purified MBP tag MBP tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey purified purified Q13635-1:p.Asp513Tyr D513Y mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D Y Tue Jun 30 19:25:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp513Tyr' Q13635-1:p.Leu282Gln L282Q mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L Q Wed Sep 26 10:24:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu282Glu' Tue Jun 30 17:21:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu282Gln' flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q13635-1:p.[Leu282Gln;Thr500Phe;Pro504Leu] L282Q/T500F/P504L mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L Q certain certain sequence position certain certain certain sequence position certain T F certain certain sequence position certain certain certain sequence position certain P L Tue Sep 04 19:19:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu282Glu;Thr500Phe;Pro504Leu]' Tue Jun 30 17:28:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu282Gln;Thr500Phe;Pro504Leu]' Q13635-1:p.[Thr500Phe;Pro504Leu] T500F/P504L mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T F certain certain sequence position certain certain certain sequence position certain P L Tue Jun 30 18:44:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr500Phe;Pro504Leu]' Q13635-1:p.Gly509Val G509V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G V Tue Jun 30 20:25:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly509Val' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Fig. 5A