DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the adenosine-bound human adenosine A1 receptor-Gi complex. Structure of the adenosine-bound human adenosine A1 receptor-Gi complex. Nature 2018 imex curation Draper-Joyce CJ., Khoshouei M., Thal DM., Liang YL., Nguyen ATN., Furness SGB., Venugopal H., Baltos JA., Plitzko JM., Danev R., Baumeister W., May LT., Wootten D., Sexton PM., Glukhova A., Christopoulos A. alisa.glukhova@monash.edu, arthur.christopoulos@monash.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Draper-Joyce CJ., Khoshouei M., Thal DM., Liang YL., Nguyen ATN., Furness SGB., Venugopal H., Baltos JA., Plitzko JM., Danev R., Baumeister W., May LT., Wootten D., Sexton PM., Glukhova A., Christopoulos A. alisa.glukhova@monash.edu, arthur.christopoulos@monash.edu Accepted 2018-SEP-11 AT 12:24 BST AT 12:24 BST by ORCHARD Nature 2018 imex curation Structure of the adenosine-bound human adenosine A1 receptor-Gi complex. Structure of the adenosine-bound human adenosine A1 receptor-Gi complex. Nature 2018 imex curation Draper-Joyce CJ., Khoshouei M., Thal DM., Liang YL., Nguyen ATN., Furness SGB., Venugopal H., Baltos JA., Plitzko JM., Danev R., Baumeister W., May LT., Wootten D., Sexton PM., Glukhova A., Christopoulos A. alisa.glukhova@monash.edu, arthur.christopoulos@monash.edu 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Draper-Joyce CJ., Khoshouei M., Thal DM., Liang YL., Nguyen ATN., Furness SGB., Venugopal H., Baltos JA., Plitzko JM., Danev R., Baumeister W., May LT., Wootten D., Sexton PM., Glukhova A., Christopoulos A. alisa.glukhova@monash.edu, arthur.christopoulos@monash.edu Accepted 2018-SEP-11 AT 12:24 BST AT 12:24 BST by ORCHARD Nature 2018 imex curation acm4_human Muscarinic acetylcholine receptor M4 CHRM4 protein protein human Homo sapiens Human MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR DIP protein P08173 original sequence version: 142 9C1B05B359A64289 gbg2_human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 G gamma-I GNG2 protein protein human Homo sapiens Human MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL DIP protein P59768 original sequence version: 58 DIP protein P59768 original sequence version: 107 EDB74E4135E7A37A p04899-1 Isoform 1 of Guanine nucleotide-binding protein G(i) subunit alpha-2 GNAI2 GNAI2B protein protein human Homo sapiens Human MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF BE6D6FF720CF3DCC p62873-1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 GNB1 Transducin beta chain 1 protein protein human Homo sapiens Human MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN 896CBD32D2686598 adora1-gper1-1 1 3 unspecified role unspecified role neutral component neutral component purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range hist tag hist tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 unspecified role unspecified role neutral component neutral component purified purified P04899-1:p.[Ser47Asn;Gly204Ala;Glu246Ala;Ala327Ser] S47N, G204A, A327S, E246A mutation mutation certain certain sequence position certain certain certain sequence position certain S N certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain A S certain certain sequence position certain certain certain sequence position certain E A Tue Sep 04 18:57:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser47Asn;Gly204Ala;Ala327Ser;Glu246Ala]' Tue Jun 30 18:15:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser47Asn;Gly204Ala;Glu246Ala;Ala327Ser]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association Fig 1., Extended Data Fig. 3, Extended Data Table 1 chrm4-gnai2-1 2 3 unspecified role unspecified role neutral component neutral component purified purified flag tag flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range hist tag hist tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role neutral component neutral component purified purified trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 unspecified role unspecified role neutral component neutral component purified purified P04899-1:p.[Ser47Asn;Gly204Ala;Glu246Ala;Ala327Ser] S47N, G204A, A327S, E246A mutation mutation certain certain sequence position certain certain certain sequence position certain S N certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain A S certain certain sequence position certain certain certain sequence position certain E A Tue Sep 04 16:18:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser47Asn;Gly204Ala;Ala327Ser;Glu246Ala]' Tue Jun 30 20:22:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser47Asn;Gly204Ala;Glu246Ala;Ala327Ser]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association Fig 1., Extended Data Fig. 3, Extended Data Table 1