IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ lncRNA MALAT1/miR-205-5p axis regulates MPP+-induced cell apoptosis in MN9D cells by directly targeting LRRK2. lncRNA MALAT1/miR-205-5p axis regulates MPP+-induced cell apoptosis in MN9D cells by directly targeting LRRK2. American journal of translational research 2017 imex curation Chen Q., Huang X., Li R. rjliwh@yeah.net 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-mn9d Mus musculus MN9D neuronal cells mn9d Murine immortalized dopamine-containing neuronal mesencephalic hybrid mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay qrt-pcr quantitative reverse transcription pcr QRT-PCR Chen Q., Huang X., Li R. the target RNA is not luciferase tagged so the expression is not measured with luciferase assay but with qrtpcr rjliwh@yeah.net Accepted 2018-MAY-24 AT 10:32 BST AT 10:32 BST by LIVIAP American journal of translational research 2017 Only protein-protein interactions imex curation imex curation lncRNA MALAT1/miR-205-5p axis regulates MPP+-induced cell apoptosis in MN9D cells by directly targeting LRRK2. lncRNA MALAT1/miR-205-5p axis regulates MPP+-induced cell apoptosis in MN9D cells by directly targeting LRRK2. American journal of translational research 2017 imex curation Chen Q., Huang X., Li R. rjliwh@yeah.net 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-mn9d Mus musculus MN9D neuronal cells mn9d Murine immortalized dopamine-containing neuronal mesencephalic hybrid mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Chen Q., Huang X., Li R. rjliwh@yeah.net Accepted 2018-MAY-24 AT 10:32 BST AT 10:32 BST by LIVIAP American journal of translational research 2017 Only protein-protein interactions imex curation imex curation hsa-mir-205 5p micro RNA 205 mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UCCUUCAUUCCACCGGAGUCUG mrna_lrrk2 mrna messenger rna mRNA mrna human Homo sapiens Human GCGCTGGCTGCGGGCGGTGAGCTGAGCTCGCCCCCGGGGAGCTGTGGCCGGCGCCCCTGCCGGTTCCCTGAGCAGCGGACGTTCATGCTGGGAGGGCGGCGGGTTGGAAGCAGGTGCCACCATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAGGACGAGGAAACTCTGAAGAAGTTGATAGTCAGGCTGAACAATGTCCAGGAAGGAAAACAGATAGAAACGCTGGTCCAAATCCTGGAGGATCTGCTGGTGTTCACGTACTCCGAGCACGCCTCCAAGTTATTTCAAGGCAAAAATATCCATGTGCCTCTGTTGATCGTCTTGGACTCCTATATGAGAGTCGCGAGTGTGCAGCAGGTGGGTTGGTCACTTCTGTGCAAATTAATAGAAGTCTGTCCAGGTACAATGCAAAGCTTAATGGGACCCCAGGATGTTGGAAATGATTGGGAAGTCCTTGGTGTTCACCAATTGATTCTTAAAATGCTAACAGTTCATAATGCCAGTGTAAACTTGTCAGTGATTGGACTGAAGACCTTAGATCTCCTCCTAACTTCAGGTAAAATCACCTTGCTGATATTGGATGAAGAAAGTGATATTTTCATGTTAATTTTTGATGCCATGCACTCATTTCCAGCCAATGATGAAGTCCAGAAACTTGGATGCAAAGCTTTACATGTGCTGTTTGAGAGAGTCTCAGAGGAGCAACTGACTGAATTTGTTGAGAACAAAGATTATATGATATTGTTAAGTGCGTTAACAAATTTTAAAGATGAAGAGGAAATTGTGCTTCATGTGCTGCATTGTTTACATTCCCTAGCGATTCCTTGCAATAATGTGGAAGTCCTCATGAGTGGCAATGTCAGGTGTTATAATATTGTGGTGGAAGCTATGAAAGCATTCCCTATGAGTGAAAGAATTCAAGAAGTGAGTTGCTGTTTGCTCCATAGGCTTACATTAGGTAATTTTTTCAATATCCTGGTATTAAACGAAGTCCATGAGTTTGTGGTGAAAGCTGTGCAGCAGTACCCAGAGAATGCAGCATTGCAGATCTCAGCGCTCAGCTGTTTGGCCCTCCTCACTGAGACTATTTTCTTAAATCAAGATTTAGAGGAAAAGAATGAGAATCAAGAGAATGATGATGAGGGGGAAGAAGATAAATTGTTTTGGCTGGAAGCCTGTTACAAAGCATTAACGTGGCATAGAAAGAACAAGCACGTGCAGGAGGCCGCATGCTGGGCACTAAATAATCTCCTTATGTACCAAAACAGTTTACATGAGAAGATTGGAGATGAAGATGGCCATTTCCCAGCTCATAGGGAAGTGATGCTCTCCATGCTGATGCATTCTTCATCAAAGGAAGTTTTCCAGGCATCTGCGAATGCATTGTCAACTCTCTTAGAACAAAATGTTAATTTCAGAAAAATACTGTTATCAAAAGGAATACACCTGAATGTTTTGGAGTTAATGCAGAAGCATATACATTCTCCTGAAGTGGCTGAAAGTGGCTGTAAAATGCTAAATCATCTTTTTGAAGGAAGCAACACTTCCCTGGATATAATGGCAGCAGTGGTCCCCAAAATACTAACAGTTATGAAACGTCATGAGACATCATTACCAGTGCAGCTGGAGGCGCTTCGAGCTATTTTACATTTTATAGTGCCTGGCATGCCAGAAGAATCCAGGGAGGATACAGAATTTCATCATAAGCTAAATATGGTTAAAAAACAGTGTTTCAAGAATGATATTCACAAACTGGTCCTAGCAGCTTTGAACAGGTTCATTGGAAATCCTGGGATTCAGAAATGTGGATTAAAAGTAATTTCTTCTATTGTACATTTTCCTGATGCATTAGAGATGTTATCCCTGGAAGGTGCTATGGATTCAGTGCTTCACACACTGCAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGTTTAAGTCTTATAGGATACTTGATTACAAAGAAGAATGTGTTCATAGGAACTGGACATCTGCTGGCAAAAATTCTGGTTTCCAGCTTATACCGATTTAAGGATGTTGCTGAAATACAGACTAAAGGATTTCAGACAATCTTAGCAATCCTCAAATTGTCAGCATCTTTTTCTAAGCTGCTGGTGCATCATTCATTTGACTTAGTAATATTCCATCAAATGTCTTCCAATATCATGGAACAAAAGGATCAACAGTTTCTAAACCTCTGTTGCAAGTGTTTTGCAAAAGTAGCTATGGATGATTACTTAAAAAATGTGATGCTAGAGAGAGCGTGTGATCAGAATAACAGCATCATGGTTGAATGCTTGCTTCTATTGGGAGCAGATGCCAATCAAGCAAAGGAGGGATCTTCTTTAATTTGTCAGGTATGTGAGAAAGAGAGCAGTCCCAAATTGGTGGAACTCTTACTGAATAGTGGATCTCGTGAACAAGATGTACGAAAAGCGTTGACGATAAGCATTGGGAAAGGTGACAGCCAGATCATCAGCTTGCTCTTAAGGAGGCTGGCCCTGGATGTGGCCAACAATAGCATTTGCCTTGGAGGATTTTGTATAGGAAAAGTTGAACCTTCTTGGCTTGGTCCTTTATTTCCAGATAAGACTTCTAATTTAAGGAAACAAACAAATATAGCATCTACACTAGCAAGAATGGTGATCAGATATCAGATGAAAAGTGCTGTGGAAGAAGGAACAGCCTCAGGCAGCGATGGAAATTTTTCTGAAGATGTGCTGTCTAAATTTGATGAATGGACCTTTATTCCTGACTCTTCTATGGACAGTGTGTTTGCTCAAAGTGATGACCTGGATAGTGAAGGAAGTGAAGGCTCATTTCTTGTGAAAAAGAAATCTAATTCAATTAGTGTAGGAGAATTTTACCGAGATGCCGTATTACAGCGTTGCTCACCAAATTTGCAAAGACATTCCAATTCCTTGGGGCCCATTTTTGATCATGAAGATTTACTGAAGCGAAAAAGAAAAATATTATCTTCAGATGATTCACTCAGGTCATCAAAACTTCAATCCCATATGAGGCATTCAGACAGCATTTCTTCTCTGGCTTCTGAGAGAGAATATATTACATCACTAGACCTTTCAGCAAATGAACTAAGAGATATTGATGCCCTAAGCCAGAAATGCTGTATAAGTGTTCATTTGGAGCATCTTGAAAAGCTGGAGCTTCACCAGAATGCACTCACGAGCTTTCCACAACAGCTATGTGAAACTCTGAAGAGTTTGACACATTTGGACTTGCACAGTAATAAATTTACATCATTTCCTTCTTATTTGTTGAAAATGAGTTGTATTGCTAATCTTGATGTCTCTCGAAATGACATTGGACCCTCAGTGGTTTTAGATCCTACAGTGAAATGTCCAACTCTGAAACAGTTTAACCTGTCATATAACCAGCTGTCTTTTGTACCTGAGAACCTCACTGATGTGGTAGAGAAACTGGAGCAGCTCATTTTAGAAGGAAATAAAATATCAGGGATATGCTCCCCCTTGAGACTGAAGGAACTGAAGATTTTAAACCTTAGTAAGAACCACATTTCATCCCTATCAGAGAACTTTCTTGAGGCTTGTCCTAAAGTGGAGAGTTTCAGTGCCAGAATGAATTTTCTTGCTGCTATGCCTTTCTTGCCTCCTTCTATGACAATCCTAAAATTATCTCAGAACAAATTTTCCTGTATTCCAGAAGCAATTTTAAATCTTCCACACTTGCGGTCTTTAGATATGAGCAGCAATGATATTCAGTACCTACCAGGTCCCGCACACTGGAAATCTTTGAACTTAAGGGAACTCTTATTTAGCCATAATCAGATCAGCATCTTGGACTTGAGTGAAAAAGCATATTTATGGTCTAGAGTAGAGAAACTGCATCTTTCTCACAATAAACTGAAAGAGATTCCTCCTGAGATTGGCTGTCTTGAAAATCTGACATCTCTGGATGTCAGTTACAACTTGGAACTAAGATCCTTTCCCAATGAAATGGGGAAATTAAGCAAAATATGGGATCTTCCTTTGGATGAACTGCATCTTAACTTTGATTTTAAACATATAGGATGTAAAGCCAAAGACATCATAAGGTTTCTTCAACAGCGATTAAAAAAGGCTGTGCCTTATAACCGAATGAAACTTATGATTGTGGGAAATACTGGGAGTGGTAAAACCACCTTATTGCAGCAATTAATGAAAACCAAGAAATCAGATCTTGGAATGCAAAGTGCCACAGTTGGCATAGATGTGAAAGACTGGCCTATCCAAATAAGAGACAAAAGAAAGAGAGATCTCGTCCTAAATGTGTGGGATTTTGCAGGTCGTGAGGAATTCTATAGTACTCATCCCCATTTTATGACGCAGCGAGCATTGTACCTTGCTGTCTATGACCTCAGCAAGGGACAGGCTGAAGTTGATGCCATGAAGCCTTGGCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTGTGATTCTCGTTGGCACACATTTGGATGTTTCTGATGAGAAGCAACGCAAAGCCTGCATGAGTAAAATCACCAAGGAACTCCTGAATAAGCGAGGGTTCCCTGCCATACGAGATTACCACTTTGTGAATGCCACCGAGGAATCTGATGCTTTGGCAAAACTTCGGAAAACCATCATAAACGAGAGCCTTAATTTCAAGATCCGAGATCAGCTTGTTGTTGGACAGCTGATTCCAGACTGCTATGTAGAACTTGAAAAAATCATTTTATCGGAGCGTAAAAATGTGCCAATTGAATTTCCCGTAATTGACCGGAAACGATTATTACAACTAGTGAGAGAAAATCAGCTGCAGTTAGATGAAAATGAGCTTCCTCACGCAGTTCACTTTCTAAATGAATCAGGAGTCCTTCTTCATTTTCAAGACCCAGCACTGCAGTTAAGTGACTTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAATCATGGCACAGATTTTGACAGTGAAAGTGGAAGGTTGTCCAAAACACCCTAAGGGCATTATTTCGCGTAGAGATGTGGAAAAATTTCTTTCAAAAAAAAGGAAATTTCCAAAGAACTACATGTCACAGTATTTTAAGCTCCTAGAAAAATTCCAGATTGCTTTGCCAATAGGAGAAGAATATTTGCTGGTTCCAAGCAGTTTGTCTGACCACAGGCCTGTGATAGAGCTTCCCCATTGTGAGAACTCTGAAATTATCATCCGACTATATGAAATGCCTTATTTTCCAATGGGATTTTGGTCAAGATTAATCAATCGATTACTTGAGATTTCACCTTACATGCTTTCAGGGAGAGAACGAGCACTTCGCCCAAACAGAATGTATTGGCGACAAGGCATTTACTTAAATTGGTCTCCTGAAGCTTATTGTCTGGTAGGATCTGAAGTCTTAGACAATCATCCAGAGAGTTTCTTAAAAATTACAGTTCCTTCTTGTAGAAAAGGCTGTATTCTTTTGGGCCAAGTTGTGGACCACATTGATTCTCTCATGGAAGAATGGTTTCCTGGGTTGCTGGAGATTGATATTTGTGGTGAAGGAGAAACTCTGTTGAAGAAATGGGCATTATATAGTTTTAATGATGGTGAAGAACATCAAAAAATCTTACTTGATGACTTGATGAAGAAAGCAGAGGAAGGAGATCTCTTAGTAAATCCAGATCAACCAAGGCTCACCATTCCAATATCTCAGATTGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAATATTATGTTGAATAATGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCTCCTAGGTGATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAGAAGAAGTGGCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTAAGACAAGAGCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATCTTTGCTGGCAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCTCCAAGGGTTCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACTAGAACCCTACAGCACAGGATTGCACTCCACGTAGCTGATGGTTTGAGATACCTCCACTCAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGCTGCTTTTCACACTGTATCCCAATGCTGCCATCATTGCAAAGATTGCTGACTACGGCATTGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGGCACACCAGGGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAGGCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTGACAACTGGAGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGATGAATTAGAAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTTGTGCCCCATGGCCTATGGTTGAGAAATTAATTAAACAGTGTTTGAAAGAAAATCCTCAAGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAATTAGTCTGTCTGACGAGACGCATTTTATTACCTAAAAACGTAATTGTTGAATGCATGGTTGCTACACATCACAACAGCAGGAATGCAAGCATTTGGCTGGGCTGTGGGCACACCGACAGAGGACAGCTCTCATTTCTTGACTTAAATACTGAAGGATACACTTCTGAGGAAGTTGCTGATAGTAGAATATTGTGCTTAGCCTTGGTGCATCTTCCTGTTGAAAAGGAAAGCTGGATTGTGTCTGGGACACAGTCTGGTACTCTCCTGGTCATCAATACCGAAGATGGGAAAAAGAGACATACCCTAGAAAAGATGACTGATTCTGTCACTTGTTTGTATTGCAATTCCTTTTCCAAGCAAAGCAAACAAAAAAATTTTCTTTTGGTTGGAACCGCTGATGGCAAGTTAGCAATTTTTGAAGATAAGACTGTTAAGCTTAAAGGAGCTGCTCCTTTGAAGATACTAAATATAGGAAATGTCAGTACTCCATTGATGTGTTTGAGTGAATCCACAAATTCAACGGAAAGAAATGTAATGTGGGGAGGATGTGGCACAAAGATTTTCTCCTTTTCTAATGATTTCACCATTCAGAAACTCATTGAGACAAGAACAAGCCAACTGTTTTCTTATGCAGCTTTCAGTGATTCCAACATCATAACAGTGGTGGTAGACACTGCTCTCTATATTGCTAAGCAAAATAGCCCTGTTGTGGAAGTGTGGGATAAGAAAACTGAAAAACTCTGTGGACTAATAGACTGCGTGCACTTTTTAAGGGAGGTAATGGTAAAAGAAAACAAGGAATCAAAACACAAAATGTCTTATTCTGGGAGAGTGAAAACCCTCTGCCTTCAGAAGAACACTGCTCTTTGGATAGGAACTGGAGGAGGCCATATTTTACTCCTGGATCTTTCAACTCGTCGACTTATACGTGTAATTTACAACTTTTGTAATTCGGTCAGAGTCATGATGACAGCACAGCTAGGAAGCCTTAAAAATGTCATGCTGGTATTGGGCTACAACCGGAAAAATACTGAAGGTACACAAAAGCAGAAAGAGATACAATCTTGCTTGACCGTTTGGGACATCAATCTTCCACATGAAGTGCAAAATTTAGAAAAACACATTGAAGTGAGAAAAGAATTAGCTGAAAAAATGAGACGAACATCTGTTGAGTAAGAGAGAAATAGGAATTGTCTTTGGATAGGAAAATTATTCTCTCCTCTTGTAAATATTTATTTTAAAAATGTTCACATGGAAAGGGTACTCACATTTTTTGAAATAGCTCGTGTGTATGAAGGAATGTTATTATTTTTAATTTAAATATATGTAAAAATACTTACCAGTAAATGTGTATTTTAAAGAACTATTTAAAACACAATGTTATATTTCTTATAAATACCAGTTACTTTCGTTCATTAATTAATGAAAATAAATCTGTGAAGTACCTAATTTAAGTACTCATACTAAAATTTATAAGGCCGATAATTTTTTGTTTTCTTGTCTGTAATGGAGGTAAACTTTATTTTAAATTCTGTGCTTAAGACAGGACTATTGCTTGTCGATTTTTCTAGAAATCTGCACGGTATAATGAAAATATTAAGACAGTTTCCCATGTAATGTATTCCTTCTTAGATTGCATCGAAATGCACTATCATATATGCTTGTAAATATTCAAATGAATTTGCACTAATAAAGTCCTTTGTTGGTATGTGAATTCTCTTTGTTGCTGTTGCAAACAGTGCATCTTACACAACTTCACTCAATTCAAAAGAAAACTCCATTAAAAGTACTAATGAAAAAACATGACATACTGTCAAAGTCCTCATATCTAGGAAAGACACAGAAACTCTCTTTGTCACAGAAACTCTCTGTGTCTTTCCTAGACATAATAGAGTTGTTTTTCAACTCTATGTTTGAATGTGGATACCCTGAATTTTGTATAATTAGTGTAAATACAGTGTTCAGTCCTTCAAGTGATATTTTTATTTTTTTATTCATACCACTAGCTACTTGTTTTCTAATCTGCTTCATTCTAATGCTTATATTCATCTTTTCCCTAAATTTGTGATGCTGCAGATCCTACATCATTCAGATAGAAACCTTTTTTTTTTTCAGAATTATAGAATTCCACAGCTCCTACCAAGACCATGAGGATAAATATCTAACACTTTTCAGTTGCTGAAGGAGAAAGGAGCTTTAGTTATGATGGATAAAAATATCTGCCACCCTAGGCTTCCAAATTATACTTAAATTGTTTACATAGCTTACCACAATAGGAGTATCAGGGCCAAATACCTATGTAATAATTTGAGGTCATTTCTGCTTTAGGAAAAGTACTTTCGGTAAATTCTTTGGCCCTGACCAGTATTCATTATTTCAGATAATTCCCTGTGATAGGACAACTAGTACATTTAATATTCTCAGAACTTATGGCATTTTACTATGTGAAAACTTTAAATTTATTTATATTAAGGGTAATCAAATTCTTAAAGATGAAAGATTTTCTGTATTTTAAAGGAAGCTATGCTTTAACTTGTTATGTAATTAACAAAAAAATCATATATAATAGAGCTCTTTGTTCCAGTGTTATCTCTTTCATTGTTACTTTGTATTTGCAATTTTTTTTACCAAAGACAAATTAAAAAAATGAATACCATATTTAAATGGAATAATAAAGGTTTTTTAAAAACTTTAAAAAAAAAAAAAAAAA lncrn_malat1 mouse lncrna long non-coding ribonucleic acid lncRNA long non coding RNA long non coding ribonucleic acid long non-coding RNA lncrna mouse Mus musculus Mouse AGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTACTTAGTCTTAATAATCTAAGAAGATCTTAAGAGATAACATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAATGAGGAGAAAAGTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTTAAAACAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAATCCTTCGACTGGCGCATGTACGTTTGAAGGCATGAGTTGGAAACAGGGAAGATGGAAGTGTTAGGCTAGCCGGGCGATGGTGGCGCACGCCTTTAATCCTAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGAAAAAACAAAAAGGTTAGGCTAGTATTTGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAGACATACAGGAAGGTGAAGAAAAAGCTGTTAGAGAAGATAGGAAAATAGAAGACAAAGCATCTTTAGAAGACAGAAAAGGTACTTAAAGGCACAGGTAGTAGGAAGCCGAAGAATAGAAGATAGAAAGAAGCAAGATAGAAAAACAAAATGGAAGTTAAGACAACTTTGGATGCCAGCATTCAAGATAGGCAAAGAAGATAAGATTGAGGCCAAAAGGTTGGATAAGATATAAAGTCAGAAGGAAATTATCTTTAAAGCCATAAGTTCAAATTTCTGATGGAGCGAGCAGTTTAGAAGAGTCTTTAGACAGCCACATACAAGATTGAAGCTAGCAATCAAAGCTACTAGGACTGAAGTAAAAAGTTAAGGCAGAATGCCTTTGAAGAGTTAGAAGAATATTAAAAGCCTTAACTTGTAGCTTAATTTTGCTTGATGACAAAAGGACTTTTGATAACAGTTTCAAGATTGTCAGCATTTTGCATTGGACTTGAGCTGAGGTGCTTTTAAAATCCTAACGACTAGCATTGGCAGCTGACCCAGGTCTACACAGAAGTGCATTCAGTGAACTAGGAAGACAGGAGCGGCAGACAGGAGTCCCGAAGCCAGTTTGGTGAAGCTAGGAAGGACTGAGGAGCCAGCAGCAGCAGTGCATGGTGAAGATAGCCCAGGAAAGAGTGCGGTTCGGTGGAGGAAGCTAGGAAGAAGGAGCCATACGGATGTGGTGGTGAAGCTGGGAAAGGGTTCCAGGATGGTGGAGCGAGAGCGAGTTGGTGATGAAGCTAGCTGGCGGCTTGGCTTGTCAACTGCGCGGAGGAGGCGAGCAGGCATTGTGGAGAGGATAGATAGCGGCTCCTAGACCAGCATGCCAGTGTGCAAGAAAGGCTGCAGGGAGAGCATGCGGTGCGGTAACATTCCTTGAGGTCGGCAACATGGTGGTGGTTTTCTGTAACTTGGATGGTAACTTGTTTACTTTGTCTTAATAGTTATGGGGGAGTTGTAGGCTTCTGTGTAAAGAGATATATCTGGGGCTGTATGTAGGCCTTTGCGGGTGTTGTAGGTTTTTCTTTTTCAGGGTTATGTCCTCTTGCATCTTGTCAGAAGCTTTTGAGGGCTGACTGCCAAGGCCCAGAAAGAAGAATGGTAGATGGCAAGTTGTCTTTAACCGCTCAGAGGGGAATGAATGGTAGAGCCAGCACAACCTCCCAGTTTTGTAAGACGTTGTAGTTTGAACAGATGACCTACCACAAGCCTCACTCCTGTGTAGGGGAGGTAATTGGGCAAAGTGCTTTTGGGGGAATGGGGGCAAAATATATTTTGAGTTCTTTTCCCCTTAGGTCTGTCTAGAATCCTAAAGGCAGATGACTCAAGGGAACCAGAAAAAAGGAAATCCACTCTCAGGATAAGCAGAGCTCGCCAGGTTTACAGTTTGTAGGAAGTAGAGGATGGATGCTAGCTTTCACACTGAGTGTGGAGGAGCTGGCCATGGCGGAATTGCTGGTAGTTTACTCTTTCCCCCTCCCTTAATGAGATTTGTAAAATCCTAAACACTTTTACTTGAAATATTTGGGAGTGGTCTTAACAGGGAGGAGTGGGTGGGGGAAACGTTTTTTTTCTAAGATTTTCCACAGATGCTATAGTTGTGTTGACACACTGGGTTAGAGAAGGCGTGTACTGCTATGCTGTTGGCACGACACCTTCAGGGACTGGAGCTGCCTTTTGTCCTTGGAAGAGTTTTCCCAGTTGCCGCTGAAGTCAGCACAGTGCGGCTTTGGTTCACAGTCACCTCAGGAGAACCTCAGGAGCTTGGCTAGGCCAGAGGTTGAAGTTAAGTTTTACAGCACCGTGATTTAAAATATTTCATTAAAGGGGAGGGGTAAAACTTAGTTGGCTGTGGCCTTGTGTTTGGGTGGGTGGGGGTGTTAGGTAATTGTTTAGTTTATGATTTCAGATAATCATACCAGAGAACTTAAATATTTGGAAAAACAGGAAATCTCAGCTTTCAAGTTGGCAAGTAACTCCCAATCCAGTTTTTGCTTCTTTTTTCCTTTTTCTTTTTTTGAGGCGGGCAGCTAAGGAAGGTTGGTTCCTCTGCCGGTCCCTCGAAAGCGTAGGGCTTGGGGGTTGGTCTGGTCCACTGGGATGATGTGATGCTACAGTGGGGACTCTTCTGAAGCTGTTGGATGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAATTTCTTCTGGTGATAGCTTTTATATTATCAAGTAAGAGATACTATCTCAGTTTTGTATAAGCAAGTCTTTTTCCTAGTGTAGGAGAAATGATTTTCCTTGTGACTAAACAAGATGTAAAGGTATGCTTTTTTTCTTCTTGTGCATTGTATACTTGTGTTTATTTGTAACTTATAATTTAAGAATTATGATAATTCAGCCTGAATGTCTTTTAGAGGGTGGGCTTTTGTTGATGAGGGAGGGGAAACCTTTTTTTTTCTGTAGACCTTTTTCAGATAACACCATCTGAGTCATAACCAGCCTGGCAGTGTGATGACGTAGATGCAGAGGGAGCAGCTCCTTGGTGAATGAGTGATAAGTAAAGGCAGAAAAAATAATGTCATGTCTCCATGGGGAATGAGCATGAGCCAGAGATTGTTCCTACTGATGAAAAGCTGCATATGCAAAAATTTAAGCAAATGAAAGCAACCAGTATAAAGTTATGGCAATACCTTTAAAAGTTATGGCTTATCTACCAAGCTTTATCCACAAAAGTAAAGAATTGATGAAAAACAGTGAAGATCAAATGTTCATCTCAAAACTGCTTTTACAAAAGCAGAATAGAAATGAAGTGAAAATGCTGCATTAAGCCTGGAGTAAAAAGAAGCTGAGCTTGTTGAGATGAGTGGGATCGAGCGGCTGCGAGGCGGTGCAGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTTTCTCTTCATAAGCAGAAGAGTTGCTTCATTCCATCTCGGAGCAGGAAACAGCAGACTGCTGTTGACAGATAAGTGTAACTTGGATCTGCAGTATTGCATGTTAGGGATAGATAAGTGCCTTTTTTCTCTTTTTCCAAAAAGACCTGTAGAGCTGTTGAATGTTTGCAGCTGGCCCCTCTTAGGCAGTTCAGAATTTTGAGTAGTTTTCCCATCCAGCCTCTTAAAAATTCCTAAGCCTTGCACCGATGGGCTTTCATGATGGGATAGCTAATAGGCTTTTGCATCGTAAACTTCAACACAAAAGCCTACATGATTAATGCCTACTTTAATTACATTGCTTACAAGATTAAGGAATCTTTATCTTGAAGACCCCATGAAAGGGATCATTATGTGCTGAAAATTAGATGTTCATATTGCTAAAATTTAAATGTGCTCCAATGTACTTGTGCTTAAAATCATTAAATTATACAAATTAATAAAATACTTCACTAGAGAATGTATGTATTTAGAAGGCTGTCTCCTTATTTAAATAAAGTCTTGTTTGTTGTCTGTAGTTAGTGTGGGCAATTTTGGGGGGATGTTCTTCTCTAATCTTTTCAGAAACTTGACTTCGAACACTTAAGTGGACCAGATCAGGATTTGAGCCAGAAGACCGAAATTAACTTTAAGGCAGGAAAGACAAATTTTATTCTCCATGCAGTGATGAGCATTTAATAATTGCAGGCCTGGCATAGAGGCCGTCTAACTAAGGACTAAGTACCTTAGGCAGGTGGGAGATGATGGTCAGAGTAAAAGGTAACTACATATTTTGTTTCCAGAAAGTCAGGGGTCTAATTTGACCATGGCTAAACATCTAGGGTAAGACACTTTTCCCCCACATTTCCAAATATGCATGTTGAGTTTAAATGCTTACGATCATCTCATCCACTTTAGCCTTTTGTCACCTCACTTGAGCCACGAGTGGGGTCAGGCATGTGGGTTTAAAGAGTTTTCCTTTGCAGAGCCTCATTTCATCCTTCATGGAGCTGCTCAGGACTTTGCATATAAGCGCTTGCCTCTGTCTTCTGTTCTGCTAGTGAGTGTGTGATGTGAGACCTTGCAGTGAGTTTGTTTTTCCTGGAATGTGGAGGGAGGGGGGGATGGGGCTTACTTGTTCTAGCTTTTTTTTTACAGACCACACAGAATGCAGGTGTCTTGACTTCAGGTCATGTCTGTTCTTTGGCAAGTAATATGTGCAGTACTGTTCCAATCTGCTGCTATTAGAATGCATTGTGACGCGACTGGAGTATGATTAAAGAAAGTTGTGTTTCCCCAAGTGTTTGGAGTAGTGGTTGTTGGAGGAAAAGCCATGAGTAACAGGCTGAGTGTTGAGGAAATGGCTCTCTGCAGCTTTAAGTAACCCGTGTTTGTGATTGGAGCCGAGTCCCTTTGCTGTGCTGCCTTAGGTAAATGTTTTTGTTCATTTCTGGTGAGGGGGGTTGGGAGCACTGAAGCCTTTAGTCTCTTCCAGATTCAACTTAAAATCTGACAAGAAATAAATCAGACAAGCAACATTCTTGAAGAAATTTTAACTGGCAAGTGGAAATGTTTTGAACAGTTCCGTGGTCTTTAGTGCATTATCTTTGTGTAGGTGTTCTCTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACATTGACAACAGCAGACATCTATCTATTCAAGGGGCCAGAGAATCCAGACCCAGTAAGGAAAAATAGCCCATTTACTTTAAATCGATAAGTGAAGCAGACATGCCATTTTCAGTGTGGGGATTGGGAAGCCCTAGTTCTTTCAGATGTACTTCAGACTGTAGAAGGAGCTTCCAGTTGAATTGAAATTCACCAGTGGACAAAATGAGGACAACAGGTGAACGAGCCTTTTCTTGTTTAAGATTAGCTACTGGTAATCTAGTGTTGAATCCTCTCCAGCTTCATGCTGGAGCAGCTAGCATGTGATGTAATGTTGGCCTTGGGGTGGAGGGGTGAGGTGGGCGCTAAGCCTTTTTTTAAGATTTTTCAGGTACCCCTCACTAAAGGCACTGAAGGCTTAATGTAGGACAGCGGAGCCTTCCTGTGTGGCAAGAATCAAGCAAGCAGTATTGTATCGAGACCAAAGTGGTATCATGGTCGGTTTTGATTAGCAGTGGGGACTACCCTACCGTAACACCTTGTTGGAATTGAAGCATCCAAAGAAAATACTTGAGAGGCCCTGGGCTTGTTTTAACATCTGGAAAAAAGGCTGTTTTTATAGCAGCGGTTACCAGCCCAAACCTCAAGTTGTGCTTGCAGGGGAGGGAAAAGGGGGAAAGCGGGCAACCAGTTTCCCCAGCTTTTCCAGAATCCTGTTACAAGGTCTCCCCACAAGTGATTTCTCTGCCACATCGCCACCATGGGCCTTTGGCCTAATCACAGACCCTTCACCCCTCACCTTGATGCAGCCAGTAGCTGGATCCTTGAGGTCACGTTGCATATCGGTTTCAAGGTAACCATGGTGCCAAGGTCCTGTGGGTTGCACCAGAAAAGGCCATCAATTTTCCCCTTGCCTGTAATTTAACATTAAAACCATAGCTAAGATGTTTTATACATAGCACCTATGCAGAGTAAACAAACCAGTATGGGTATAGTATGTTTGATACCAGTGCTGGGTGGGAATGTAGGAAGTCGGATGAAAAGCAAGCCTTTGTAGGAAGTTGTTGGGGTGGGATTGCAAAAATTCTCTGCTAAGACTTTTTCAGGTGGACATAACAGACTTGGCCAAGCTAGCATCTTAGTGGAAGCAGATTCGTCAGTAGGGTTGTAAAGGTTTTTCTTTTCCTGAGAAAACAACCTTTTGTTTTCTCAGGTTTTGCTTTTTGGCCTTTCCCTAGCTTTAAAAAAAAAAAAGCAAAAGACGCTGGTGGCTGGCACTCCTGGTTTCCAGGACGGGGTTCAAGTCCCTGCGGTGTCTTTGCTTGACTCTTATATCATGAGGCCATTACATTTTTCTTGGAGGGTTCTAAAGGCTCTGGGTATGGTAGCTGATATCACTGGAACACTCCCCAGCCTCAGTGTTGAACTCTTGATAATTAACTGCATTGTCTTTCAGGTTATGCCCAATTCGTCTTATTACCTCTGAGTCGACACACCTCCTACTATTTATTGAATACTTTGATTTTATGAAATAAAAACTAAATATCTCTCA hsa_mir_205 5p-mrna_lrrk2-1 2 4 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined r.7821_7828delinscgagtccg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain ATGAAGGA CGAGTCCG 3 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered physical association physical association Fig 3A,B hsa_mir_205 5p-lncrn_malat1 human-1 1 5 regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.[g1531_1540delinscaacgcagta; a3657_g3665delinsgcatccatg] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain AATGAAGTG GCATCCATG certain certain sequence position certain certain certain sequence position certain GGTGATGAAG CAACGCAGTA g1531_1540delinscaacgcagta mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GGTGATGAAG CAACGCAGTA a3657_g3665delinsgcatccatg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AATGAAGTG GCATCCATG 3 predetermined predetermined participant predetermined qrt-pcr quantitative reverse transcription pcr QRT-PCR regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered physical association physical association Fig 2A, B