UniProt UniProt http://www.uniprot.org Direct interaction between selenoprotein R and Aβ42. Direct interaction between selenoprotein R and Aβ42. Biochemical and biophysical research communications 2017 imex curation Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn Accepted 2019-MAR-05 AT 10:11 GMT AT 10:11 GMT by PPORRAS Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation Direct interaction between selenoprotein R and Aβ42. Direct interaction between selenoprotein R and Aβ42. Biochemical and biophysical research communications 2017 imex curation Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease A FRET signal was measured following photo-bleaching. The change in donor fluorescence induced by acceptor photo-bleaching was quantified by comparing pre-bleach and post-bleach images giving a measure both of FRET efficiency and the distance between the interactive proteins. Source of APP peptide confirmed by author liuqiong@szu.edu.cn Accepted 2019-MAR-05 AT 10:11 GMT AT 10:11 GMT by PPORRAS Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation Direct interaction between selenoprotein R and Aβ42. Direct interaction between selenoprotein R and Aβ42. Biochemical and biophysical research communications 2017 imex curation Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn Accepted 2019-MAR-05 AT 10:11 GMT AT 10:11 GMT by PPORRAS Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 msrb1_human Methionine-R-sulfoxide reductase B1 MSRB1 SEPX1 Selenoprotein X HSPC270 protein protein human Homo sapiens Human MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRFUIFSSSLKFVPKGKETSASQGH 812B033BE02B7C0E app-msrb1-1 2 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 1 To verify the interaction between SelR′ and Aβ42, FRET method of receptor photobleaching was performed. app-msrb1-2 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2 Co-IP assays were performed to further confirm the interaction between SelR′ and Aβ42. Interaction confirmed by anti-bait western msrb1-app-1 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2 Co-IP assays were performed to further confirm the interaction between SelR′ and Aβ42. Interaction confirmed by anti-bait western msrb1-app-2 1 5 unspecified role unspecified role bait bait purified purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey purified purified region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Fig 3 To corroborate the interaction between SelR′ and Aβ42, we performed pull-down assay on the recombinant His tagged SelR′ in combination with Aβ42, which obtained from the cell lysis of HEK293T cells transfected with CFP-Aβ42 (cells transfected with pECFP-C1 as negative control) by incubating with anti-GFP microbeads. Interaction confirmed by anti-bait western