UniProt UniProt http://www.uniprot.org Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Scientific Reports 2017 imex curation Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp Accepted 2018-JUL-11 AT 15:56 BST AT 15:56 BST by PPORRAS Scientific Reports 2017 Only protein-protein interactions imex curation imex curation Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Scientific Reports 2017 imex curation Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp Accepted 2018-JUL-11 AT 15:56 BST AT 15:56 BST by PPORRAS Scientific Reports 2017 Only protein-protein interactions imex curation imex curation Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Scientific Reports 2017 imex curation Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro footprinting footprinting predetermined predetermined participant predetermined Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp Accepted 2018-JUL-11 AT 15:56 BST AT 15:56 BST by PPORRAS Scientific Reports 2017 Only protein-protein interactions imex curation imex curation Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor. Scientific Reports 2017 imex curation Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Goto-Ito S., Yamagata A., Takahashi TS., Sato Y., Fukai S. fukai@iam.u-tokyo.ac.jp Accepted 2018-JUL-11 AT 15:56 BST AT 15:56 BST by PPORRAS Scientific Reports 2017 Only protein-protein interactions imex curation imex curation q9h7e2-3 Tudor domain-containing protein 3 TDRD3 protein protein human Homo sapiens Human MAQVAGAALSQAGWYLSDEGIEACTSSPDKVNVNDIILIALNTDLRTIGKKFLPSDINSGKVEKLEGPCVLQIQKIRNVAAPKDNEESQAAPRMLRLQMTDGHISCTAVEFSYMSKISLNTPPGTKVKLSGIVDIKNGFLLLNDSNTTVLGGEVEHLIEKWELQRSLSKHNRSNIGTEGGPPPFVPFGQKCVSHVQVDSRELDRRKTLQVTMPVKPTNDNDEFEKQRTAAIAEVAKSKETKTFGGGGGGARSNLNMNAAGNRNREVLQKEKSTKSEGKHEGVYRELVDEKALKHITEMGFSKEASRQALMDNGNNLEAALNVLLTSNKQKPVMGPPLRGRGKGRGRIRSEDEEDLGNARPSAPSTLFDFLESKMGTLNVEEPKSQPQQLHQGQYRSSNTEQNGVKDNNHLRHPPRNDTRQPRNEKPPRFQRDSQNSKSVLEGSGLPRNRGSERPSTSSVSEVWAEDRIKCDRPYSRYDRTKDTSYPLGSQHSDGAFKKRDNSMQSRSGKGPSFAEAKENPLPQGSVDYNNQKRGKRESQTSIPDYFYDRKSQTINNEAFSGIKIEKHFNVNTDYQNPVRSNSFIGVPNGEVEMPLKGRRIGPIKPAGPVTAVPCDDKIFYNSGPKRRSGPIKPEKILESSIPMEYAKMWKPGDECFALYWEDNKFYRAEVEALHSSGMTAVVKFIDYGNYEEVLLSNIKPIQTEAWEEEGTYDQTLEFRRGGDGQPRRSTRPTQQFYQPPRARN CF27720025EFBD81 top3b_human DNA topoisomerase 3-beta-1 TOP3B TOP3B1 DNA topoisomerase III beta-1 protein protein human Homo sapiens Human MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV 75532827856CFF8F tdrd3-top3b-1 2 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 9 11 direct interaction direct interaction 3.61A R working 0.198, R free 0.245 Supp. Table 1 Here we report the crystal structures of the catalytic domain of TOP3β, the DUF1767–OB-fold domains of TDRD3 and their complex at 3.44 Å, 1.62 Å and 3.6 Å resolutions, respectively. top3b-tdrd3-1 4 5 unspecified role unspecified role prey prey purified purified Q9H7E2-3:p.[Val109Ser;Phe111Ser] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V S certain certain sequence position certain certain certain sequence position certain F S Wed May 02 15:40:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val109ser,phe111ser' Tue Jun 30 17:25:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val109Ser;Phe111Ser]' Q9H7E2-3:p.Val109Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V S Wed May 02 15:18:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val109ser' Tue Jun 30 15:52:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val109Ser' Q9H7E2-3:p.Phe111Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F S Wed May 02 19:59:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe111ser' Tue Jun 30 17:34:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe111Ser' Q9H7E2-3:p.[Pro92Ser;Met94Ser] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P S certain certain sequence position certain certain certain sequence position certain M S Wed May 02 18:11:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro92ser,met94ser' Tue Jun 30 17:47:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro92Ser;Met94Ser]' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9H7E2-3:p.Pro82Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P S Wed May 02 18:05:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro82ser' Tue Jun 30 18:31:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro82Ser' Q9H7E2-3:p.Arg96Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:12:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96ala' Tue Jun 30 18:27:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg96Ala' Q9H7E2-3:p.Phe139Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F V Wed May 02 16:55:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe139val' Tue Jun 30 18:55:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe139Val' Q9H7E2-3:p.[Arg96Met;Val109Met] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain V M Wed May 02 16:13:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,val109met' Tue Jun 30 18:48:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Val109Met]' Q9H7E2-3:p.[Arg96Met;Phe139Val] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain F V Wed May 02 16:44:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,phe139val' Tue Jun 30 18:07:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Phe139Val]' Q9H7E2-3:p.Val79Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V S Wed May 02 15:15:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val79ser' Tue Jun 30 18:14:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val79Ser' Q9H7E2-3:p.[Val79Ser;Pro82Ser] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V S certain certain sequence position certain certain certain sequence position certain P S Wed May 02 17:00:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val79ser,pro82ser' Tue Jun 30 18:05:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val79Ser;Pro82Ser]' Q9H7E2-3:p.Ala80_Ala81delinsSerSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AA SS Wed May 02 18:23:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala_ala80-81ser_ser' Tue Jun 30 17:53:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala80_Ala81delinsSerSer' Q9H7E2-3:p.Val79_Pro82delinsSerSerSerSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VAAP SSSS Mon Jan 22 16:57:51 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ala_ala79-81ser_ser_ser,pro82ser' Wed May 02 20:33:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ala_ala_pro79-82ser_ser_ser_ser' Tue Jun 30 18:07:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val79_Pro82delinsSerSerSerSer' Q9H7E2-3:p.Pro92Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P S Wed May 02 17:31:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro92ser' Tue Jun 30 19:18:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92Ser' Q9H7E2-3:p.Met94Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M S Wed May 02 20:26:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met94ser' Tue Jun 30 17:35:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met94Ser' Q9H7E2-3:p.Arg96Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R M Wed May 02 15:50:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met' Tue Jun 30 20:09:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg96Met' Q9H7E2-3:p.[Phe139Ser;Leu141Ser] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F S certain certain sequence position certain certain certain sequence position certain L S Wed May 02 19:21:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe139ser,leu141ser' Tue Jun 30 17:36:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe139Ser;Leu141Ser]' Q9H7E2-3:p.Leu141Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L S Wed May 02 17:25:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu141ser' Tue Jun 30 18:36:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu141Ser' Q9H7E2-3:p.Phe139Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F S Wed May 02 16:57:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe139ser' Tue Jun 30 19:49:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe139Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9H7E2-3:p.[Ala80_Ala81delinsSerGln;Ala90_Pro92delinsLysProSer] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain AAP KPS certain certain sequence position certain certain certain sequence position certain AA SQ Tue Mar 13 13:06:34 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala80ser,ala81gln,ala90lys,ala91pro,pro92ser' Wed May 02 15:24:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala_ala80-81ser_gln,ala_ala_pro90-92lys_pro_ser' Tue Jun 30 19:04:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ala80_Ala81delinsSerGln;Ala90_Pro92delinsLysProSer]' Q9H7E2-3:p.Val109Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Wed May 02 18:11:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val109met' Tue Jun 30 17:31:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val109Met' Q9H7E2-3:p.Phe111Tyr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F Y Wed May 02 17:10:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe111tyr' Tue Jun 30 20:15:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe111Tyr' Q9H7E2-3:p.[Val109Met;Phe111Tyr;Phe139Val] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain V M certain certain sequence position certain certain certain sequence position certain F V Wed May 02 20:11:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val109met,phe111tyr,phe139val' Tue Jun 30 18:35:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val109Met;Phe111Tyr;Phe139Val]' Q9H7E2-3:p.[Arg96Met;Phe111Tyr] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain R M Wed May 02 16:17:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,phe111tyr' Tue Jun 30 19:47:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Phe111Tyr]' Q9H7E2-3:p.[Arg96Met;Val109Met;Phe111Tyr] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain V M certain certain sequence position certain certain certain sequence position certain F Y Wed May 02 15:11:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,val109met,phe111tyr' Tue Jun 30 20:13:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Val109Met;Phe111Tyr]' Q9H7E2-3:p.[Arg96Met;Val109Met;Phe139Val] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain V M certain certain sequence position certain certain certain sequence position certain F V Wed May 02 19:54:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,val109met,phe139val' Tue Jun 30 18:05:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Val109Met;Phe139Val]' Q9H7E2-3:p.[Arg96Met;Phe111Tyr;Phe139Val] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F V certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain F Y Wed May 02 16:42:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,phe111tyr,phe139val' Tue Jun 30 19:43:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Phe111Tyr;Phe139Val]' Q9H7E2-3:p.[Arg96Met;Val109Met;Phe111Tyr;Phe139Val] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R M certain certain sequence position certain certain certain sequence position certain V M certain certain sequence position certain certain certain sequence position certain F Y certain certain sequence position certain certain certain sequence position certain F V Wed May 02 20:19:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96met,val109met,phe111tyr,phe139val' Tue Jun 30 18:16:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg96Met;Val109Met;Phe111Tyr;Phe139Val]' 6 unspecified role unspecified role bait bait purified purified O95985:p.Asp266Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:10:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp266ala' Tue Jun 30 19:43:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp266Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 34 46 direct interaction direct interaction Fig. 3C, 3F, 3G, 4B, 4D, Supp. Figs 5, 7 we tested binding of TOP3β or TDRD3 mutants by GST pull-down assays. tdrd3-top3b-2 1 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. Fig. 3A Our binding analysis using the size-exclusion column chromatography showed that the TDRD3 OB-fold domain alone could bind TOP3β (Supplementary Fig. 3a). tdrd3-top3b-3 3 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Supp. Fig. 3B