UniProt UniProt http://www.uniprot.org Molecular mechanism of the allosteric regulation of the αγ heterodimer of human NAD-dependent isocitrate dehydrogenase. Molecular mechanism of the allosteric regulation of the αγ heterodimer of human NAD-dependent isocitrate dehydrogenase. Scientific reports 2017 imex curation Ma T., Peng Y., Huang W., Ding J. jpding@sibcb.ac.cn 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ma T., Peng Y., Huang W., Ding J. jpding@sibcb.ac.cn Accepted 2018-MAR-12 AT 11:30 GMT AT 11:30 GMT by MGT Scientific reports 2017 imex curation adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) isocitrate(2-) isocitrate(2-) hydrogen isocitrate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) idh3a_human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial IDH3A Isocitric dehydrogenase subunit alpha NAD(+)-specific ICDH subunit alpha protein protein human Homo sapiens Human MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 695F6A34F97430CF citrate anion citrate anion citrate citrate anions small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) idh3g_human Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial IDH3G Isocitric dehydrogenase subunit gamma NAD(+)-specific ICDH subunit gamma protein protein human Homo sapiens Human MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA A0870F2B7D228A37 magnesium(2+) Magnesium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) idh3a-idh3g-1 1 7 unspecified role unspecified role unspecified role unspecified role 2 unspecified role unspecified role unspecified role unspecified role 5 cofactor cofactor coenzyme unspecified role unspecified role 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 15 direct interaction direct interaction Table 1 2.65A Rworking 0.195 Rfree 0.241 adp-idh3a-1 1 3 cofactor cofactor coenzyme unspecified role unspecified role 7 unspecified role unspecified role unspecified role unspecified role 2 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 23 21 direct interaction direct interaction Table 1 2.79A Rworking 0.226 Rfree 0.274 adp-citrate anion-1 1 7 unspecified role unspecified role unspecified role unspecified role 2 unspecified role unspecified role unspecified role unspecified role 5 cofactor cofactor coenzyme unspecified role unspecified role 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51553:p.Lys190Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:13:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys190ala' Tue Jun 30 15:50:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys190Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 29 32 direct interaction direct interaction Table 1 2.5A Rworking 0.207 Rfree 0.248 citrate anion-idh3a-1 1 7 unspecified role unspecified role unspecified role unspecified role 5 cofactor cofactor coenzyme unspecified role unspecified role 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 37 39 direct interaction direct interaction Table 1 2.31A Rworking 0.241 Rfree 0.271 idh3a-idh3g-2 1 6 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 44 42 direct interaction direct interaction Table 1 2.8A Rworking 0.224 Rfree 0.259