DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Nature structural & molecular biology 2016 imex curation Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Accepted 2020-JUN-23 AT 12:02 BST AT 12:02 BST by MGT Nature structural & molecular biology 2016 Only protein-protein interactions imex curation imex curation Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Nature structural & molecular biology 2016 imex curation Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Accepted 2020-JUN-23 AT 12:02 BST AT 12:02 BST by MGT Nature structural & molecular biology 2016 Only protein-protein interactions imex curation IMEx imex curation Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Nature structural & molecular biology 2016 imex curation Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) bn-page blue native page bn-page experimental particp experimental participant identification experimental particp Mir-Sanchis I., Roman CA., Misiura A., Pigli YZ., Boyle-Vavra S., Rice PA. price@uchicago.edu Accepted 2020-JUN-23 AT 12:02 BST AT 12:02 BST by MGT Nature structural & molecular biology 2016 Only protein-protein interactions imex curation imex curation q0wxp6_staau EQ90_14515 R114_17 R92_17 SA950122_00035 GO814_14830 DQU54_00225 GO942_10655 SAMEA103891420_02329 protein protein staau Staphylococcus aureus MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNARFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQLMRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTFDNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKSTAAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGLLYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQFTTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNTGIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQFVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVNALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNWTPSNDNKAAKELFKSANEGIGPSGVHEDF A7E9E297B0F5815F q0wxp6_staau-1 2 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2, Table 1 q0wxp6_staau-2 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Sup Fig 5a, 5c q0wxp6_staau-4 3 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Sup Fig 5d