IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Proteins 2014 imex curation Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in Accepted 2018-JAN-16 AT 09:12 GMT AT 09:12 GMT by LIVIAP Proteins 2014 imex curation added in CLIC1; I have removed it! Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Proteins 2014 imex curation Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in Accepted 2018-JAN-16 AT 09:12 GMT AT 09:12 GMT by LIVIAP Proteins 2014 imex curation added in CLIC1; I have removed it! Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Proteins 2014 imex curation Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. For the Peptide inhibition assays His-gankyrin was pre-incubated with peptides before being added to GST-CLIC1 bound to glutathione beads. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in Accepted 2018-JAN-16 AT 09:12 GMT AT 09:12 GMT by LIVIAP Proteins 2014 imex curation added in CLIC1; I have removed it! Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Proteins 2014 imex curation Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer It is stated that:- 'Lysates of cells were added to gankyrin bound and IgG bound Sepharose G beads followed by immunoblotting with (various antibodies.)' However the expression system is not stated for gankyrin, so it has been entered here as His-tagged and from E.coli, as this is the most probable source. there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Text under "Experimental validation of predicted interactions" states:- anti-gankyrin antibody was immobilized on protein G Sepharose beads and used to to pull down endogenous complexes... Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in Accepted 2018-JAN-16 AT 09:12 GMT AT 09:12 GMT by LIVIAP Proteins 2014 imex curation added in CLIC1; I have removed it! Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Discovery of multiple interacting partners of gankyrin, a proteasomal chaperone and an oncoprotein--evidence for a common hot spot site at the interface and its functional relevance. Proteins 2014 imex curation Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nanaware PP., Ramteke MP., Somavarapu AK., Venkatraman P. Cancer - Interactions investigated in the context of cancer there is an Excel spreadsheet in the Supp.materials with a list of ~260 proteins; I have no idea what this list is for/of. Gankyrin and HSP70 were found to be of Human origin by primer Blat - see Table S1. Other constructs notably CLIC1, NCK2, GRSF1 and S6ATPase cDNA are stated as being of Human origin. vprasanna@actrec.gov.in Accepted 2018-JAN-16 AT 09:12 GMT AT 09:12 GMT by LIVIAP Proteins 2014 imex curation added in CLIC1; I have removed it! mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV DIP protein Q02750 original sequence version: 96 DIP protein Q02750 original sequence version: 93 DIP protein Q02750-1 original sequence version: 151 DIP protein Q02750 original sequence version: 151 DIP protein Q02750-1 original sequence version: 166 0344118FFC842D51 clic1_human Chloride intracellular channel protein 1 CLIC1 NCC27 Nuclear chloride ion channel 27 Chloride channel ABP Regulatory nuclear chloride ion channel protein G6 protein protein human Homo sapiens Human MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK 163EEB7481826A0A psd10_human 26S proteasome non-ATPase regulatory subunit 10 26S proteasome regulatory subunit p28 Gankyrin PSMD10 p28(GANK) protein protein human Homo sapiens Human MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG DIP protein O75832 original sequence version: 149 57158E33146EC7C8 prs6b_human 26S proteasome regulatory subunit 6B Proteasome 26S subunit ATPase 4 MB67-interacting protein PSMC4 TBP7 MIP224 Tat-binding protein 7 26S proteasome AAA-ATPase subunit RPT3 protein protein human Homo sapiens Human MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK DIP protein P43686 original sequence version: 63 80F9523C61B88F0C nck2_human Cytoplasmic protein NCK2 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta NCK2 GRB4 Growth factor receptor-bound protein 4 protein protein human Homo sapiens Human MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ 3A211085E46F4452 grsf1_human G-rich sequence factor 1 GRSF1 protein protein human Homo sapiens Human MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAGSIPSGVSGRRRLLLLLGAAAAAASQTRGLQTGPVPPGRLAGPPAVATSAAAAAAASYSALRASLLPQSLAAAAAVPTRSYSQESKTTYLEDLPPPPEYELAPSKLEEEVDDVFLIRAQGLPWSCTMEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDVDALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPEMANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSYKGKKIASFPTAKYITEPEMVFEEHEVNEDIQPMTAFESEKEIELPKEVPEKLPEAADFGTTSSLHFVHMRGLPFQANAQDIINFFAPLKPVRITMEYSSSGKATGEADVHFETHEDAVAAMLKDRSHVHHRYIELFLNSCPKGK E09EE4E70212A70F ddah1_human N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 DDAH1 DDAH DDAHI Dimethylargininase-1 protein protein human Homo sapiens Human MAGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLINKKVDS DIP protein O94760 original sequence version: 140 CD8875DF267EF39B hspa1a_hspa1b_human-1 HSPA1A_HSPA1B_human molecule set molecule set human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD P0DMV8,P0DMV9 hs71a_human Heat shock 70 kDa protein 1A HSPA1A HSPA1 HSX70 Heat shock 70 kDa protein 1 HSP72 protein protein human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD 78F513118C96DE66 eevd_peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide human Homo sapiens Human EEVD psmd10-ddah1-1 1 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association figure 2A HSP90 (HSP90AB1 and/or HSP90AA1) were also detected. psmd10-ddah1-2 4 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association figure 2C HSP90 (HSP90AB1 and/or HSP90AA1) were also detected. psmd10-ddah1-3 4 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 8 unspecified role unspecified role bait bait purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association figure 2D HSP90 (HSP90AB1 and/or HSP90AA1) were also detected. psmd10-ddah1-4 4 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_435 Human cell line derived from the M14 melanoma cell line mda_mb_435 Human cell line derived from the M14 melanoma cell line This cell line is now thought to be derived from the M14 melanoma cell line. This cell line was originally thought to be a Human epithelial breast cancer cell line. see pmid: 17004106 8 unspecified role unspecified role bait bait purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association figure 2E HSP90 (HSP90AB1 and/or HSP90AA1) were also detected. psmd10-clic1-1 3 14 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DMV8:p.[Glu638_Glu639delinsAlaAla;Asp641Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:05:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu638-639ala_ala,asp641ala' Tue Jun 30 17:30:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu638_Glu639delinsAlaAla;Asp641Ala]' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction figure 3A psmd10-clic1-2 3 7 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O00299:p.[Glu150_Glu151delinsAlaAla;Asp153Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:41:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu150-151ala_ala,asp153ala' Tue Jun 30 16:29:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu150_Glu151delinsAlaAla;Asp153Ala]' O00299:p.Val152Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V E Wed May 02 19:13:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val152glu' Tue Jun 30 18:40:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val152Glu' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction figure 3B, Supp 3C clic1-1 2 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O00299:p.[Glu150_Glu151delinsAlaAla;Asp153Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:45:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu150-151ala_ala,asp153ala' Tue Jun 30 17:43:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu150_Glu151delinsAlaAla;Asp153Ala]' 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association figure 3C hspa1a-psmd10-1 2 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DMV8:p.[Glu638_Glu639delinsAlaAla;Asp641Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:15:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu638-639ala_ala,asp641ala' Tue Jun 30 15:41:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu638_Glu639delinsAlaAla;Asp641Ala]' physical association physical association figure 3D hspa1a-psmd10-2 2 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43639:p.[Glu167_Glu168delinsAlaAla;Asp170Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:13:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu167-168ala_ala,asp170ala' Tue Jun 30 19:39:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu167_Glu168delinsAlaAla;Asp170Ala]' physical association physical association figure 3E nck2-psmd10-1 2 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q12849:p.[Glu144_Glu145delinsAlaAla;Asp147Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:06:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu144-145ala_ala,asp147ala' Tue Jun 30 18:46:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu144_Glu145delinsAlaAla;Asp147Ala]' physical association physical association figure 3F clic1-psmd10-1 3 15 inhibitor inhibitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction figure 4 (Supp. fig 4) Supp fig 4 text states that "Band intensities of CLIC1 bound gankyrin following IP" - I assume they must use IP here in a general sense rather than as a specific interaction detection method, as there is no other mention of peptide competition elsewhere in the Methods section, or images. psmd10-clic1-3 4 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma 8 unspecified role unspecified role bait bait purified purified n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association figure 5A HSP90 (HSP90AB1 and/or HSP90AA1) were also detected. clic1-psmd10-2 5 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O00299:p.[Glu150_Glu151delinsAlaAla;Asp153Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:22:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu150-151ala_ala,asp153ala' Tue Jun 30 17:49:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu150_Glu151delinsAlaAla;Asp153Ala]' physical association physical association figure 6A clic1-psmd10-3 1 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O00299:p.[Glu150_Glu151delinsAlaAla;Asp153Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:07:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu150-151ala_ala,asp153ala' Tue Jun 30 20:01:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu150_Glu151delinsAlaAla;Asp153Ala]' physical association physical association figure 6C clic1-psmd10-4 1 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P43686:p.[Glu356_Glu357delinsAlaAla;Asp359Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:43:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu356-357ala_ala,asp359ala' Tue Jun 30 19:12:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu356_Glu357delinsAlaAla;Asp359Ala]' physical association physical association Supp. figure 2C nck2-psmd10-2 1 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43639:p.[Glu167_Glu168delinsAlaAla;Asp170Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:23:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu167-168ala_ala,asp170ala' Tue Jun 30 19:36:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu167_Glu168delinsAlaAla;Asp170Ala]' physical association physical association Supp figure 3A grsf1-psmd10-1 1 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q12849:p.[Glu144_Glu145delinsAlaAla;Asp147Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain EE AA certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:47:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_glu144-145ala_ala,asp147ala' Tue Jun 30 18:10:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu144_Glu145delinsAlaAla;Asp147Ala]' physical association physical association Supp figure 3B