IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Mouse lung cDNA library (Clontech) Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lung Mus musculus lung anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Bronchoalveolar lavage (BAL) fluids from IL-13 Tg mice: cell types unknown, probably mainly macrophages, lymphocytes, neutrophils plus other immune cells. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lung Mus musculus lung confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. IL-13 transgenic (Tg) mice in which Chi3l1/BRP-39 and IL-13Ra2 are both strongly induced. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lung Mus musculus lung anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Lung lysates were prepared from WT and IL-13 transgenic mice. The IL13 transgene allows conditional expression of IL13 inducible by doxycycline; Chi3l1/BRP-39 and IL-13Ra2 are also strongly induced. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-lung_epithelium Mouse lung epithelial cells lung_epithelium lung epithelial cells fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Type 2 alveolar epithelial cells from IL-13 Tg mice, in which Chi3l1/BRP-39 and IL-13Ra2 are both strongly induced. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Figure 1 E/F constructs have been assumed to all be of Human origin based on molecule length and correspondance of domain numbering. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Peritoneal macrophages isolated from IL-13Ra2−/− mice jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-macrophage Mus musculus macrophage macrophage Macrophage fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. there is NO BTO term for "lung-macrophage" so not created as a "tissue". Lung macrophages from IL-13 Tg mice, in which Chi3l1/BRP-39 and IL-13Ra2 are both strongly induced. jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Chitinase 3-like 1 regulates cellular and tissue responses via IL-13 receptor α2. Cell reports 2013 imex curation He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. jack_elias@brown.edu 25-Jul-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot He CH., Lee CG., Dela Cruz CS., Lee CM., Zhou Y., Ahangari F., Ma B., Herzog EL., Rosenberg SA., Li Y., Nour AM., Parikh CR., Schmidt I., Modis Y., Cantley L., Elias JA. Peritoneal macrophages from Chi3l1−/− mice jack_elias@brown.edu Accepted 2019-MAY-23 AT 08:04 BST AT 08:04 BST by ORCHARD Cell reports 2013 Only protein-protein interactions imex curation imex curation altered to Physiological as well here; altered; done; Go terms removed + altered Exp. Prep to Physiological; have altered Exp.Prep. to Physiological.; interaction moved to Expt-43 il13_mouse Interleukin-13 T-cell activation protein P600 Il13 Il-13 protein protein mouse Mus musculus Mouse MALWVTAVLALACLGGLAAPGPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF 954F93F105713FED il13_human Interleukin-13 IL13 NC30 protein protein human Homo sapiens Human MHPLLNPLLLALGLMALLLTTVIALTCLGGFASPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN DIP protein P35225 original sequence version: 112 BE2D46CCF913D129 i13r2_mouse Interleukin-13 receptor subunit alpha-2 Il13ra2 protein protein mouse Mus musculus Mouse MAFVHIRCLCFILLCTITGYSLEIKVNPPQDFEILDPGLLGYLYLQWKPPVVIEKFKGCTLEYELKYRNVDSDSWKTIITRNLIYKDGFDLNKGIEGKIRTHLSEHCTNGSEVQSPWIEASYGISDEGSLETKIQDMKCIYYNWQYLVCSWKPGKTVYSDTNYTMFFWYEGLDHALQCADYLQHDEKNVGCKLSNLDSSDYKDFFICVNGSSKLEPIRSSYTVFQLQNIVKPLPPEFLHISVENSIDIRMKWSTPGGPIPPRCYTYEIVIREDDISWESATDKNDMKLKRRANESEDLCFFVRCKVNIYCADDGIWSEWSEEECWEGYTGPDSKIIFIVPVCLFFIFLLLLLCLIVEKEEPEPTLSLHVDLNKEVCAYEDTLC C25212325C47E35B ch3l1_mouse Chitinase-3-like protein 1 Breast regression protein 39 Chil1 Chi3l1 Brp39 Cartilage glycoprotein 39 BRP39 protein protein protein mouse Mus musculus Mouse MHTSTEARMGMRAALTGFAVLMLLQSCSAYKLVCYFTSWSQYREGVGSFLPDAIQPFLCTHIIYSFANISSDNMLSTWEWNDESNYDKLNKLKTRNTNLKTLLSVGGWKFGEKRFSEIASNTERRTAFVRSVAPFLRSYGFDGLDLAWLYPRLRDKQYFSTLIKELNAEFTKEVQPGREKLLLSAALSAGKVAIDTGYDIAQIAQHLDFINLMTYDFHGVWRQITGHHSPLFQGQKDTRFDRYSNVNYAVQYMIRLGAQASKLLMGIPTFGKSFTLASSENQLGAPISGEGLPGRFTKEAGTLAYYEICDFLKGAEVHRLSNEKVPFATKGNQWVGYEDKESVKNKVGFLKEKKLAGAMVWALDLDDFQGTCQPKEFFPLTNAIKDALA mint May play an important role in the capacity of cells to respond to and cope with changes in their environment. May play a role in tissue remodeling and defense against pathogens 9E7D66069B233834 ch3l1_human Chitinase-3-like protein 1 CHI3L1 39 kDa synovial protein Cartilage glycoprotein 39 YKL-40 protein protein human Homo sapiens Human MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT 6C9EF133BDC7C2D1 i13r2_human Interleukin-13 receptor subunit alpha-2 IL13RA2 IL13R Interleukin-13-binding protein protein protein human Homo sapiens Human MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGFILILVIFVTGLLLRKPNTYPKMIPEFFCDT DIP protein Q14627 original sequence version: 99 3C6ACB1B5562C887 chi3l1-il13ra2-13 1 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association no figure chi3l1-il13ra2-3 8 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Supplementary Figure 1A Extended Exp. Proc states "all positive constructs were rescreened"; I am assuming this is what is shown in Supp. fig 1A, but might be from screening. chi3l1-il13ra2-4 4 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Figure 3A, Supplementary Figure 1D Chi3l1 and IL-13Ra2 can be seen together on the surface and in the cytoplasm of the cell (Figures 1C and S1D). Chi3l1 and IL-13Ra2 can be seen together on the surface and in the cytoplasm of the cell (Figures 1C and S1D). chi3l1-il13ra2-5 2 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Supplementary figure 1E chi3l1-il13ra2-6 2 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Supplementary figure 1Ei 89youihkj-1 7 18 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 1A chi3l1-1 7 18 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 1A chi3l1-il13ra2-7 11 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Figure 1B chi3l1-il13ra2-8 6 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Figure 1B chi3l1-il13ra2-9 10 18 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 17 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 1C Chi3l1 and IL-13Ra2 can be seen together on the surface and in the cytoplasm of the cell (Figures 1C and S1D). chi3l1-il13ra2-14 3 18 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems 17 unspecified role unspecified role bait bait purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems direct interaction direct interaction Figure 1D The Biacore assays also demonstrated that Chi3l1/YKL-40 and IL-13Rα2 bind to one another chi3l1-il13ra2-10 8 18 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed 17 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 1E I cannot find any info as to which is Bait/Prey here chi3l1-il13ra2-11 8 18 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed physical association physical association Figure 1F I cannot find any info as to which is Bait/Prey here Four sites of N-linked glycosylation within the ECD were also able to be mutated without abrogating IL-13Ra2 ECD-Chi3l1/YKL-40 binding (Figure 1F). chi3l1-il13ra2-12 4 16 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed 15 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization Figure 3A Chi3l1, IL-13Rα2, and IL-13 colocalize in tissue sections (Figure 3A) Chi3l1, IL-13Rα2, and IL-13 colocalize in tissue sections (Figure 3A). chi3l1-il13-1 5 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 15 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 3B-1 Interaction only seen in IL-13 Tg mice, and not WT. Chi3l1, IL-13Ra2, and IL-13 participate in a multimeric complex, because the immunoprecipitation of any one of the three always immunoprecipitated the other two. Chi3l1, IL-13Ra2, and IL-13 colocalize in tissue sections (Figure 3A) and participate in a multimeric complex, because the immunoprecipitation of any one of the three always immunoprecipitated the other two il13ra2-chi3l1-1 5 16 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 3B-2 Interaction only seen in IL-13 Tg mice, and not WT. Chi3l1, IL-13Ra2, and IL-13 participate in a multimeric complex, because the immunoprecipitation of any one of the three always immunoprecipitated the other two. Chi3l1, IL-13Ra2, and IL-13 colocalize in tissue sections (Figure 3A) and participate in a multimeric complex, because the immunoprecipitation of any one of the three always immunoprecipitated the other two chi3l1-il13-2 5 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 3B-3 This interaction was seen in WT and IL-13 Tg mice. The molecule detected by an antibody to "h13119" or similar, has been assumed to mean Chi3l1. il13-il13ra2-8 12 15 unspecified role unspecified role prey prey cell lysate cell lysate mouse-macrophage Mus musculus macrophage macrophage Macrophage 14 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems physical association physical association Fig 3D Added recombinant Chi3l1 increased the amount of -iL-13Ra2 precipitated. il13ra2-chi3l1-2 12 16 unspecified role unspecified role prey prey cell lysate cell lysate mouse-macrophage Mus musculus macrophage macrophage Macrophage 15 unspecified role unspecified role bait bait cell lysate cell lysate mouse-macrophage Mus musculus macrophage macrophage Macrophage 17 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems association association Fig 3D-2 Cell lysates were treated with recombinant (Human) Chi3l1. The Chi3l1 detected here is probably a mixture of exogenous and endogenous forms. il13ra2-chi3l1-3 12 16 unspecified role unspecified role prey prey cell lysate cell lysate mouse-macrophage Mus musculus macrophage macrophage Macrophage 15 unspecified role unspecified role bait bait cell lysate cell lysate mouse-macrophage Mus musculus macrophage macrophage Macrophage association association Fig 3D-3 As no Human recombinant Chi3l1 has been added in this assay (LHS band) the endogenous form of Chi3l1 must have been detected here. chi3l1-13 9 17 unspecified role unspecified role prey prey purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-macrophage Mus musculus macrophage macrophage Macrophage physical association physical association Figure 3F-1 Deletion of amino acids corresponding to site II (IL-13Ra2dS2) and site III (IL-13Ra2dS3) of IL-13Ra2, the reported IL-13 binding sites in IL-13Ra2 (Lupardus et al., 2010 ), abrogates IL-13 binding without abrogating Ch13l1 binding (Figure 3F). I did try and work out what these deletion range for these mutants was - but exact aas involved not given clearly in stated reference, not that I could see. il13ra2-chi3l1-4 12 14 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-tni trini Cabbage looper tni tni-Trichoplusia ni ovarian cell line obtained from Expression Systems 18 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-macrophage Mus musculus macrophage macrophage Macrophage physical association physical association Figure 3F-2 Deletion of amino acids corresponding to site II (IL-13Ra2dS2) and site III (IL-13Ra2dS3) of IL-13Ra2, the reported IL-13 binding sites in IL-13Ra2 (Lupardus et al., 2010 ), abrogates IL-13 binding without abrogating Ch13l1 binding (Figure 3F). I did try and work out what these deletion range for these mutants was - but exact aas involved not given clearly in stated reference, not that I could see.